放化疗细胞保护剂WR-2721对国人血小板功能影响的研究

中国临床药理学与治疗学 ›› 2002, Vol. 7 ›› Issue (6): 518-521.

放化疗细胞保护剂WR-2721对国人血小板功能影响的研究

霍冠华, 吕耀凤¹, 高洪秋², 徐庆荣³

滨洲医学院组织胚胎学教研室,¹妇产科教研室,³药理学教研室,滨州 256603;
²山东省博兴县人民医院外科,博兴 256500

收稿日期:2002-08-02 修回日期:2002-08-16 出版日期:2002-12-26 发布日期:2020-11-20
通讯作者: 霍冠华,男,副教授,医学硕士,主要从事肿瘤分子生物学和放化疗的细胞保护研究。Tel:0543-3257049 　Fax:0543-3256843 　E-mail:jerry_huo@yahoo.com

Effects of WR-2721, a cytoprotector of chemotherapy and radiation on functions of platelet in Chinese healthy volunteers

HUO Guan-Hua, LU Yao-Feng¹, GAO Hong-Qiu², XU Qing-Rong³

Department of Human Histology &Embryology,¹Department of Gynecology &Obstetrics,³Department of Pharmacology,Binzhou Medical College,Binzhou 256603;
²Department of Surgery,People' s Hospital of Boxing County,Boxing 256500

Received:2002-08-02 Revised:2002-08-16 Online:2002-12-26 Published:2020-11-20

摘要/Abstract

摘要： 目的探讨放、化疗细胞保护剂WR -2721对生理性激活剂ADP 、胶原和血小板激活因子(PAF)引起的血小板激活的影响。方法取20 ~ 35岁健康人血液,分别给予生理性激活剂ADP 1 μmol·L^-1 、胶原2 mg·L^-1和PAF 0.1 mg·L^-1后,再用终浓度为10^-7 ~ 10^-5 mol·L^-1的WR-2721 处理,观察对血小板聚集的影响,然后观察血栓素B₂(TXB₂)和NO 的水平。结果 WR-2721 抑制ADP 、胶原和PAF诱导的血小板聚集和TXB₂ 产生,并存在剂量依赖关系。在终浓度为5 μmol·L^-1 时,WR-2721 明显增加用ADP 、胶原和PAF 诱导的NO 的产生,表明活化血小板释放的NO 参与WR-2721 的抑制效应。结论 WR-2721 体外可有效地抑制生理性激活剂引起的血小板活化。除了细胞保护作用外,WR-2721 还可以用予治疗有关的疾病。

关键词: WR-2721, 血小板激活因子, 血栓素2, 胶原, 血小板活化

Abstract: AIM: To explore in vitro the effects of WR-2721,a cytoprotector of chemotherapy and radiation,on platelet activation induced by physiologic agonists ADP,collagen and PAF.METHODS: Blood,obtained from Chinese healthy volunteers aged 20 -35 years,was treated with physiologic agonists at the given concentrations:ADp1 μmol·L^-1,collagen 2 mg·L^-1,and PAF0.1 mg·L^-1.Then WR-2721 was added to the experimental system at final concentrations range of 10^-7 -10^-5 mol·L^-1.The effect of WR-2721 on platelet aggregation induced by the agonists was studied.Thromboxane B₂(TXB₂)levels and nitric oxide(NO)production were determined.RESULTS: WR-2721 inhibited both platelet aggregation and TXB₂ production induced by ADP,collagen and PAF,in a dose-dependent manner.When WR-2721 was added at the final concentration of 5μmol·L^-1,it significantly increased ADP,collagen and PAF-induced NO production,which suggested that NO release by activated platelets was involved in the inhibitory effect of WR-2721.CONCLUSION: WR-2721 can effectively inhibit platelet activation induced in vitro by physiologic agonists.It may be not only a cytoprotectant,but also a therapeutic agent.

Key words: WR-2721, ADP, thromboxane B₂, collagen, platelet activation

中图分类号:

R969.4

霍冠华, 吕耀凤¹, 高洪秋², 徐庆荣³. 放化疗细胞保护剂WR-2721对国人血小板功能影响的研究[J]. 中国临床药理学与治疗学, 2002, 7(6): 518-521.

HUO Guan-Hua, LU Yao-Feng, GAO Hong-Qiu, XU Qing-Rong. Effects of WR-2721, a cytoprotector of chemotherapy and radiation on functions of platelet in Chinese healthy volunteers[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2002, 7(6): 518-521.

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