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中国临床药理学与治疗学 ›› 2003, Vol. 8 ›› Issue (1): 44-47.

• 研究原著 • 上一篇    下一篇

尿毒净Ⅱ号对人肾系膜细胞增殖影响的实验研究

朱晓玲, 王永钧, 程晓霞, 童孟立, 陈洪宇, 张满生   

  1. 浙江中医学院附属二院肾内科, 杭州 310007, 浙江
  • 收稿日期:2002-09-23 修回日期:2002-10-01 发布日期:2020-11-25
  • 通讯作者: 朱晓玲, 女, 副主任医师, 主要从事肾脏疾病治疗和研究。Tel:0571-85157591 E-mail:wenliyong@mail.hz.zj.cn
  • 作者简介:王永钧, 男, 教授, 主任医师, 博士生导师, 主要从事肾脏疾病治疗和研究。
  • 基金资助:
    1 由浙江省中医药管理局基金(№2000C64) 和杭州市卫生局基金(№2000A034) 资助

Effects of niaodujing Ⅱ on the proliferation of human glomerular mesangial cells in vitro

ZHU Xiao-Ling, WANG Yong-Jun, CHENG Xiao-Xia, TONG Meng-Li, ZHENG Hong-Yu, ZHANG Man-Sheng   

  1. Department of Nephrology, Second Affiliated Hospital, Zhejiang University of Chinese Traditional Medicine, Hangzhou 310007, Zhejiang
  • Received:2002-09-23 Revised:2002-10-01 Published:2020-11-25

摘要: 目的: 探讨尿毒净Ⅱ号对人肾小球系膜细胞增殖的影响。方法: 体外培养人胚胎肾小球系膜细胞, 应用氮蓝四唑盐(MTT) 比色法、流式细胞术对DNA 倍体进行分析, 分别检测尿毒净Ⅱ号对系膜细胞增殖的影响。结果: 尿毒净Ⅱ号明显抑制血清加内皮素-1(ET-1) 刺激的系膜细胞增殖(P <0.01),G0 G1 期细胞比例增加, S 期细胞比例逐渐下降, 呈一定的量效和时效依赖关系。结论: 尿毒净Ⅱ号通过抑制细胞DNA 合成, 延缓了细胞周期进程, 从而抑制了系膜细胞增殖。

关键词: 药效学, 肾脏, 系膜细胞, 增殖, 尿毒净Ⅱ号;, 内皮素-1

Abstract: AIM: To investigate the effects of niaodujing Ⅱ on cultured human glomerular mesangial cells. METHODS: The proliferation of cultured mesangial cells (MsC) in human embry, stimulated by serum and ET-1 following incubation with niaodujing Ⅱ, was measured in vitro by MTT method and flow cytomertry (FCM). RESULTS: Niaodujing Ⅱ inhibited the proliferation stimulated by serum and endothelin-1 (ET-1) in a dose-dependent and time-dependent manner (P <0.01). In the mesangial cell cycle, the number of cells in G0 G1 phase increased and the number in S phase decreased. CONCLUSION: Niaodujing Ⅱ can suppress the proliferation of MsC by inhibiting DNA synthesis.

Key words: pharmacodynamics, niaodujingⅡ, renal mesangial cells, endothelin-1, proliferation

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