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中国临床药理学与治疗学 ›› 2003, Vol. 8 ›› Issue (1): 71-74.

• 研究原著 • 上一篇    下一篇

Bosentan 对培养乳鼠心肌细胞肥大的抑制作用

王社临, 方五旺1, 陈绍良1   

  1. 马鞍山市人民医院心内科, 马鞍山 243000, 安徽; 1南京市第一医院心内科, 南京 210006, 江苏
  • 收稿日期:2002-08-24 修回日期:2002-10-24 出版日期:2003-02-26 发布日期:2020-11-25
  • 通讯作者: 王社临, 男, 副主任医师, 研究方向:心脏病电生理学和心脏介入治疗学。Tel:0555-8222308 E-mail:qinjun@yiyee.com
  • 作者简介:方五旺, 男, 医学博士。陈绍良, 男, 博士, 研究方向:心脏介入治疗。

Inhibition of bosentan on cultured cardiomyocyte hypertrophy from neonatal mice

WANG Se-Lin, FANG Wu-Wang1, CHEN Shao-Liang1   

  1. Department of Cardiology, Maanshan People Hospital, Maanshan 242000, Anhui; 1Nanjing First People Hospital, Nanjing 210006, Jiangsu
  • Received:2002-08-24 Revised:2002-10-24 Online:2003-02-26 Published:2020-11-25

摘要: 目的: 以内皮素(ET-1) 诱导培养的乳鼠心肌细胞肥大, 观察内皮素受体拮抗剂bosentan 对肥大心肌细胞的抑制作用。方法: 以胰酶消化法分离乳鼠心肌细胞, 分4 组进行细胞培养。对照组常规培养, 不加任何干预因素, 其余3 组分别加入bosentan10-8mol·L-1、ET-1 10-10mol·L-1、ET-1 10-10 +bosentan10-8mol·L-1, 培养72 h 后观察心肌细胞生长情况并用放免法测定培养液中ET-1 和心钠素(ANP) 含量。结果: 各组ANP测定水平分别为:对照组6.46 ±0.38 μg·L-1, bosentan组4.87±0.56 μg·L-1, ET-1组13.60 ±1.12 μg·L-1, ET-1 +bosentan 组9.42 ±0.58 μg·L-1。镜下观察见ET-1 刺激乳鼠心肌细胞肥大, 加用bosentan 能使心肌细胞肥大程度减轻。结论: ET-1可刺激乳鼠心肌细胞肥大, bosentan 可使心肌细胞分泌心钠素减少, 抑制ET-1诱导的心肌细胞肥大作用。

关键词: 药效学, 内皮素受体拮抗剂, 心肌细胞, bosentan

Abstract: AIM: To observe the role of endothelin antagonist bosentan that inhibits cultured neonatal mice cardiomyocyte from endothelin-1 (ET-1) induced cell hypertrophy.METHODS: Digested by trypsin, neonatal mice cardiomyocytes were isolated and cultured in 4 groups.With a group of regular culture cells as control, other three groups were respectively treated by bosentan 10 -8 mol·L -1, ET-1 10 -10 mol·L -1 andET-1 10 -10 mol·L -1 +bosentan 10 -8 mol·L -1 which were initially administered to the culture medium.The cell growing was observed carefully through micrography.72 hours later, the level of atrial natriuretic peptide (ANP) and ET-1 in the media of the 4 groups were determined by method of radioimmunoassay.RESULTS: The values of ANP in different groups were:6.46 ±0.38 μg·L -1 in control, 4.87 ±0.56 μg·L -1 in bosentan group, 13.60 ±1.12 μg·L -1 in ET-1 group and 9.42 ±0.58 μg·L -1 in ET-1 +bosentan group.Also by micrographic observation, endothelin induced cell hypertrophy was proved, and the hypertrophy could be reversed by bosentan.CONCLUSION: Endothelin induces hypertrophy of cultured neonatal mice cardiomyocytes, but it can be inhibited by endothelin antagonist of bosentan.

Key words: pharmacodynamics, endothelin, cardiomyocyte, bosentan

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