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中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (1): 67-69.

• 研究原著 • 上一篇    下一篇

盐酸埃他卡林对小鼠脑组织缺氧的保护作用

刘蔚, 龙超良, 崔文玉, 汪海   

  1. 军事医学科学院毒物药物研究所, 北京 100850
  • 收稿日期:2003-04-23 修回日期:2003-05-08 出版日期:2004-01-26 发布日期:2020-11-16
  • 通讯作者: 汪海,男, 医学博士, 研究员, 博士生导师, 研究方向:心脑血管药理和新药研究。
  • 作者简介:刘蔚, 女, 主管药师, 在读博士生, 现工作单位:解放军309 医院临床药理科。Tel:010-66775099  E-mail: weiliu528 @hotmail.com
  • 基金资助:
    军队医药卫生科研基金“ 十五” 重点项目(№01Z025)

Protection of iptakalim hydrochloride against brain anoxia in mice

LIU Wei, LONG Chao-Liang, CUI Wen-Yu, WANG Hai   

  1. Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China
  • Received:2003-04-23 Revised:2003-05-08 Online:2004-01-26 Published:2020-11-16

摘要: 目的: 研究盐酸埃他卡林(Ipt) 对脑组织缺氧的保护作用。方法: 小鼠给予Ipt 0.5, 2.0, 8.0 mg·kg-1 3个剂量(n =12, ig), 连续给药3 wk, 并设生理盐水组和维生素E 组作为对照, 采用断头制备脑缺氧模型。活性采用化学比色法测定脑组织过氧化脂质(MDA) 含量、谷胱甘肽过氧化物酶(GSH-PX) 和超氧化物歧化酶(SOD) 活性。外周红细胞膜脂流动性以DPH 为荧光探针, 采用荧光偏振法测定。结果: Ipt0.5, 2.0, 8.0 mg·kg-1 3 个剂量组延长小鼠断头后喘息持续时间, 增加外周红细胞膜脂流动性, 降低微粘度, 且呈现量效关系。相同条件下, Ipt 对MDA 含量,GSH-PX 活性及SOD 活性的影响不明显。结论: Ipt 对脑组织缺氧有显著保护作用, 其作用可能与其改变血液流变学特性有关。

关键词: 盐酸埃他卡林, 脑缺氧, 红细胞膜脂流动性, 微粘度, 过氧化脂质, 超氧化物歧化酶, 谷胱甘肽过氧化物酶

Abstract: AIM: To study the protection of iptakalim hydrochloride (Ipt) against brain anoxia in mice.METHODS: The models of brain anoxia were made in mice after the administration of Ipt (0.5, 2.0, 8.0 mg·kg-1, n =12, ig) for 3 wk.NS and vitamin E were set as controls.The glutathione proxidase (GSH-PX) activity, superoxide dismutase (SOD) activity and the lipid peroxide (MDA) content were determined in the brain homogenates of models, and the values of erythrocyte membrane fluidity were measured by the fluorescence polarization technique with DPH fluorescence probe.RESULTS: Compared with the control groups, Ipt (0.5, 2.0, and 8.0 mg·kg-1, ig) markedly prolonged the breathing time and increased the fluidity of erythrocyte membrane in a dwse-dependent manner, but had no apparent effects on the activity of GSH-PX and SOD.CONCLUSION: The protection of Ipt against brain anoxia is related to increasing the fluidity of erythrocyte membrane and decreasing blood viscosity.

Key words: iptakalim hydrochloride, brain anoxia, erythrocyte membrane fluidity, blood viscosity, MDA, SOD, GSH-PX

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