不同剂量和不同给药次数的链脲霉素对大鼠胰岛的影响

中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (10): 1128-1133.

不同剂量和不同给药次数的链脲霉素对大鼠胰岛的影响

闻智鸣

安徽中医学院第一附属医院内分泌科, 合肥230031, 安徽

收稿日期:2004-04-21 接受日期:2004-08-09 出版日期:2004-10-26 发布日期:2020-11-23

Influence of different dosages and injecting times of streptozotocin on islets of diabetic rats

WEN Zhi-Ming

Department of Endocrine, the First Affiliated Hospital, Anhui College of Chinese Traditional Medicine, Hefei 230031, Anhui, China

Received:2004-04-21 Accepted:2004-08-09 Online:2004-10-26 Published:2020-11-23
Contact: WEN Zhi-Ming, male, MD, engaged in the study of diabetes. Tel:0551-2632161 E-mail:wzm7890 @163. com

摘要/Abstract

摘要： 目的: 研究不同剂量和不同给药次数的链脲霉素(STZ) 诱发胰岛炎和损害α、β细胞的程度。方法: 将Wistar大鼠分成6组:对照组;A组:STZ 50mg·kg^-1·d^-11 次给药;B 组:STZ 50 mg·kg^-1 ·d^-连续2 d 给药;C组:STZ 50 mg·kg^-1·d^-1 连续3 d 给药;D 组:STZ 30 mg·kg^-1·d^-1 连续5 d 给药;E 组:STZ 80 mg·kg^-1·d^-1 1 次给药。观察各组大鼠的体重和末梢血糖变化, 择期摘取大鼠的胰腺制作成组织切片, 进行HE, Azan 和免疫组织化学染色, 并在光学显微镜下进行组织学比较。结果: 与对照组相比, 各组大鼠的胰腺均存在明显的胰岛炎, D 组胰岛的炎细胞浸润、空洞形成和组织纤维化较其他各组显著严重, 各组残留胰岛数量、胰岛平均面积、β 细胞面积和β 细胞面积/胰岛平均面积之比均显著减少, 尤其是C 、D 和E 组最明显;相反, 各组的α细胞面积和α细胞面积/胰岛平均面积之比因α细胞的显露而显著增大。结论: STZ 可同时诱发化学性胰岛炎和自身免疫性胰岛炎, 破坏α细胞和β 细胞, 使胰岛出现空洞形成和组织纤维化。STZ 80 mg·kg^-1 ·d^-11 次给药可造成最严重的胰岛损害。

关键词: 糖尿病大鼠, 链脲霉素, 免疫组织化学技术, 抗胰岛素抗体, 抗胰高糖素抗体

Abstract: AIM: To investigate the damage of alpha and beta cells on islets of diabetic rats in different dosages and injecting times of streptozotocin (STZ).METHODS: Wistar rats were divided into six groups: control group, group A (single injection with 50mg·kg^-1STZ), group B (injection with 50 mg·kg^-1·d^-1STZ for two days), group C (injection with 50mg·kg^-1·d^-1STZ for three days), group D (injection with 30 mg·kg^-1·d^-1 STZ for five days) and group E (single injection with 80 mg·kg^-1STZ). The body weights and concentrations of peripheral glucose of rats were measured regularly. Then the pancreases of rats were selected to make tissue slices. The tissue slices embedded in paraffin were histological observes under the light microscope after staining with Hematoxlin and Eosin (HE), Azan and immunohistochemical techniques.RESULTS: Insulitis was observed in each group throughout the term of experiment. It was more serious in all rats that were injected with STZ than those in the control group. The inflammation, vacuolization and fibrosis of islets in the group D were more serious than those in the other groups. The amount of islets, the average area of islets, the area of beta cells, the ratio of the area of beta cells and the average area of islets in each group were significantly decreased, especially in the group C, D and E. Conversely, the area of alpha cells, the ratio of the area of alpha cells and the average area of islets in each group significantly increased as alpha cells appeared.CONCLUSION: STZ can induce chemical insulitis and autoimmune insulitis, destroy both alpha and deltas cells, and make vacuolization and fibrosis in islets. Single, high dose of STZ can cause the greatest damage.

Key words: diabetic rats, streptozotocin, immunohistochemical techniques, anti-insulin antibody, anti-glucagon antibody

中图分类号:

R587

闻智鸣. 不同剂量和不同给药次数的链脲霉素对大鼠胰岛的影响[J]. 中国临床药理学与治疗学, 2004, 9(10): 1128-1133.

WEN Zhi-Ming. Influence of different dosages and injecting times of streptozotocin on islets of diabetic rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(10): 1128-1133.

参考文献

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