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中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (10): 1147-1149.

• 研究原著 • 上一篇    下一篇

白藜芦醇诱导K562 细胞凋亡过程中活性氧水平的改变

郝春燕, 苏海翔1, 魏虎来1, 张哲文   

  1. 兰州医学院生物化学与分子生物学教研室, 1医学实验中心, 兰州730000, 甘肃
  • 收稿日期:2004-07-31 修回日期:2004-09-02 出版日期:2004-10-26 发布日期:2020-11-23
  • 通讯作者: 郝春燕, 女, 硕士, 讲师, 研究方向为自由基生物学与肿瘤。Tel:0931-8289564 E-mail:chunyanhao@126. com

Changes of reactive oxygen species in apoptosis induced by resveratrol in K562 cells

HAO Chun-Yan, SU Hai-Xiang1, WEI Hu-Lai1, ZHANG Zhe-Wen   

  1. Department of Biochemistry and Molecular Biology, 1Laboratory Center for Medical Science, Lanzhou Medical College, Lanzhou 730000, Gansu, China
  • Received:2004-07-31 Revised:2004-09-02 Online:2004-10-26 Published:2020-11-23

摘要: 目的: 探讨白藜芦醇对K562 细胞的凋亡诱导效应和对K562 细胞内活性氧水平的影响。方法: 应用噻唑蓝(MTT) 比色法、光镜、DNA 琼脂糖凝胶电泳和流式细胞术(FCM) 检测细胞凋亡;FCM 测定细胞内活性氧(ROS) 水平。结果: 白藜芦醇显著抑制K562 细胞增殖, 并呈现典型的凋亡形态学改变;DNA 电泳可见梯状条带出现;FCM 分析显示S 期细胞数明显增多, 出现S/G2 期阻滞, ROS 水平升高。结论: 白藜芦醇诱导K562 细胞凋亡与细胞内活性氧水平升高有关。

关键词: 白藜芦醇, 白血病, K562, 细胞凋亡, 活性氧

Abstract: AIM: To investigate the apoptosis of K562 cells induced by resveratrol and the influence of intracellular reactive oxygen species (ROS) level.METHODS: MTT assay, light microscopy, DNA agarose gel electrophoresis and cell cycle analysis were used for examine apoptosis in K562 cells. Intracellular ROS level was measured with flow cytometry (FCM).RESULTS: Resveratrol inhibited growth of K562 cells. Agarose gel electrophoresis showed evident DNA fragmentation. Cell cycle analysis indicated increased S phase proportion, as well as apparent S/G2 phase arrest. After application of resveratrol, the level of the markedly increased of ROS.CONCLUSION: Resveratrol induces apoptosis in K562 cells may be through stimulating ROS production.

Key words: resveratrol, leukemia, K562, apoptosis, reactive oxygen species (ROS)

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