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中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (10): 1157-1159.

• 研究原著 • 上一篇    下一篇

山茶花总黄酮对缺血性脑损伤的保护作用

罗胜勇, 董六一, 范丽, 方明, 陈志武   

  1. 安徽医科大学药理学教研室, 合肥230032, 安徽
  • 收稿日期:2004-07-17 修回日期:2004-08-25 出版日期:2004-10-26 发布日期:2020-11-23
  • 通讯作者: 陈志武, 男, 博士, 教授, 博士生导师, 研究方向:神经 心血管药理学。Tel:0551-5161133 E-mail:Zhiwu. Chen @providence. org
  • 作者简介:罗胜勇, 男, 在读硕士研究生, 研究方向:神经药理学。Tel:13855137256 E-mail:lsy770728 @126.com
  • 基金资助:
    安徽自然科学基金资助(№99044433)

Protective effects of total of flavone c on cerebral ischemia injury in mice

LUO Sheng-Yong, DONG Liu-Yi, FAN Li, FANG Ming, CHEN Zhi-Wu   

  1. Department of Pharmacology, Anhui Medical University, Hefei 230032, Anhui, China
  • Received:2004-07-17 Revised:2004-08-25 Online:2004-10-26 Published:2020-11-23

摘要: 目的: 研究山茶花总黄酮(TFC) 对小鼠急性脑缺血和大鼠局灶性脑缺血的保护作用。方法: 结扎小鼠双侧颈总动脉建立脑缺血模型, 观察TFC 对小鼠死亡率及死亡时间的影响。建立局灶性脑缺血(MCAO) 模型, 观察TFC 大鼠对行为学、脑梗死面积的影响, 并测定缺血侧脑组织中乳酸脱氢酶(LDH)活性、丙二醛(MDA) 及一氧化氮(NO) 含量。结果: TFC 80 、40 mg·kg-1 组可降低缺血小鼠的死亡率, 延长死亡时间;60 、30 、15 mg·kg-1 组可改善行为障碍,降低行为学评分;60 、30 mg·kg-1 组可减少MCAO 大鼠脑梗死范围, 降低脑组织中MDA 、NO 含量, 抑制LDH 活性的下降。结论: TFC 对缺血性脑损伤有保护作用, 其机制可能与抗自由基和抑制NO 生成有关。

关键词: 山茶花总黄酮, 脑缺血, 神经功能障碍, 丙二醛, 一氧化氮

Abstract: AIM: To investigate the protective effects of total of flavone C (TFC) on acute cerebral ischemia in mice and focal cerebral ischemia in rats.METHODS: The occlusion of bilateral common carotid arteries with vagus nerves in mice was used for make the acute cerebral ischemia models. The survival time and the death rate were observed. The permanent occlusion of the proximal of the right middle cerebral artery (MCA) was used for make the focal cerebral ischemia models. The extent of neurological deficits was observed, and the infarct area was measured by NBT staining technique. The activity of LDH and the content of MDA and NO in the ischemic cerebral cortex were determined.RESULTS: TFC of 80 and 40 mg·kg-1 prolonged the survival time and decreased the death rate of mice with acute cerebral ischemia injury. TFC of 60, 30, and 15 mg·kg-1 ameliorated neurologic deficits score and the infarct size of rats with MCAO.CONCLUSION: TFC provides significant protective effects against cerebral ischemia injury.

Key words: total of flavone C, cerebral ischemia, neurological deficits, malondialdehyde, nitric oxide

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