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中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (2): 136-139.

• 研究原著 • 上一篇    下一篇

贯叶连翘提取物和盐酸赖氨酸抗疱疹病毒复方筛选

胡然, 库宝善, 张永鹤, 贾永蕊, 姚海燕, 段淑敏1   

  1. 北京大学医学部药理学系, 北京 100083;
    1中国预防医学科学院病毒学研究所诊断二室, 北京 100052
  • 收稿日期:2003-06-30 修回日期:2003-07-22 出版日期:2004-02-26 发布日期:2020-11-16
  • 通讯作者: 库宝善,男, 教授, 药理学系主任, 主要从事神经精神药理学研究。
  • 作者简介:胡然, 女, 神经精神药理学硕士。Tel:010-62091421  E-mail: hrglenda@yahoo.com

Antiviral activity of complex of Hypericum perforatum L extract and lysine hydrochloride on herpes virus

HU Ran, KU Bao-Shan, ZHANG Yong-He, JIA Yong-Rui, YAO Hai-Yan, DUAN Shu-Min1   

  1. Department of pharmacology, Peking University Health Science Center, Beijing 100083, China;
    1Number 2 Diagnostic Lab, Virology Institute, Chinese Academy of Preventive Medicine, Beijing 100052, China
  • Received:2003-06-30 Revised:2003-07-22 Online:2004-02-26 Published:2020-11-16

摘要: 目的: 评价贯叶连翘提取物和盐酸赖氨酸复方抗疱疹病毒活性, 有效复方在整体动物的安全性。方法: (1) 采用细胞病变效应法(CPE), 测定贯叶连翘提取物与盐酸赖氨酸3 种复方(分别以质量比1∶1 、1 ∶2 和2 ∶1 配比) 对Vero 细胞和2BS 细胞的细胞毒性, 以及对单纯疱疹病毒I 型(HSV-1) 和水痘-带状疱疹病毒(VZV) 的抗病毒活性。(2) 一次灌胃(ig) 给予小鼠复方(2 ∶1), 观察14 d, 测定LD50 。结果: 贯叶连翘提取物和盐酸赖氨酸3 种复方体外对HSV-1 和VZV 表现不同程度的抗病毒活性, 最小有效浓度(MIC) 和半数有效浓度(IC50) 范围分别为9.7~ 19.5 mg·L-1和6.8 ~ 9.7 mg·L-1, 测得复方(2 ∶1) 单次小鼠ig 的LD50 为11.7 g·kg-1, 95 %置信区间为6.5 ~ 17.2 g·kg-1结论: 贯叶连翘提取物和盐酸赖氨酸复方体外具有较好抗HSV-I 和VZV 作用,2 ∶1 复方属于无毒药物范围。

关键词: 贯叶连翘提取物, 盐酸赖氨酸, 复方, 单纯疱疹病毒, 水痘-带状疱疹病毒, LD50

Abstract: AIM: To study the antiviral effects of the complex of Hypericum perforatum L and lysine hydrochloride against herpes simplex virus type 1 and varicella-zoster virus (VZV), and to evaluate the LD50 of the complex in mice.METHODS: The cytotoxities to Vero cells and 2BS cells and the antiviral activities were evaluated by cytopathic effect (CPE) inhibition assay.ICR mice were administrated(ig) the complex (2 ∶1) to determine LD50.RESULTS: The complex significantly inhibited the CPE of HSV-1 and VZV.MIC ranged from 9.7 to 19.5 mg·L-1, IC50 ranged from 6.8 to 9.7 mg·L-1.LD50 of the complex with ratio 2 ∶1 was 11.7 g·kg-1 with 95 % confidence interval of 6.5-17.2 g·kg-1 in ICR mice.CONCLUSION: The complex of Hypericum perforatum L and lysine hydrochloride has good effects on inhibiting HSV-I and VZV in vitro.The complex (2 ∶1) is safe in mice.

Key words: Hypericum perforatum L, lysine hydrochloride, complex, HSV-I, VZV, LD50

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