关键词: 人胰高血糖素类多肽-1, 血糖, 胰岛素, 糖耐量实验, 葡萄糖依赖性

Abstract: AIM: To observe the effects of glucagonlike peptide-1 (rhGLP-1) (7-36) on decreasing blood glucose and stimulating the insulin secretion in mice and rabbits, and how the actions depend on the glucose levels. METHODS: After subcutaneous injection of rhGLP-1(7-36) at different doses in different animal models, the serum was collected and the values of blood glucose and insulin were measured. RESULTS: After subcutaneous injection of rhGLP-1 (7-36) at different doses in normal C57 mice and rabbits, no obvious changes of the levels of blood glucose and insulin secretion was found, which suggested rhGLP-1 (7-36) did not affect the levels of blood glucose and the action of insulin secretion.For the mice and rabbits given doses of glucose 2 and 3 g·kg^-1 respectively, rhGLP-1 (7-36) had the effects of increasing insulin secretion, and lowering the level of blood glucose strongly, which suggested rhGLP-1(7-36) had obvious effect of increasing insulin secretion depending on the level of glucose.In the glucose tolerance test (GTT), rhGLP-1 (7-36) of 16 μg·kg^-1 increased the insulin secretion of rabbit given glucose, and had the effect of lowering the glucose curve obviously, which showed the rhGLP-1(7-36) could improve the tolerance to glucose of rabbits. CONCLUSION: rhGLP-1 (7-36) has the effect of lowering the levels of glucose.However, it has no effect on the normal level of glucose.

Key words: glucagon-like peptide-1, blood glucose, insulin, GTT, dependence on the glucose