葛根素对胰岛素抵抗大鼠脂肪组织中蛋白激酶B 表达的影响

中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (7): 770-773.

葛根素对胰岛素抵抗大鼠脂肪组织中蛋白激酶B 表达的影响

李建辉, 毕会民, 甘佩珍, 汪保华¹

武汉大学人民医院内分泌科, 武汉430060, 湖北;
¹荆门市第一人民医院神经内科, 荆门448000, 湖北

收稿日期:2004-06-09 修回日期:2004-06-26 出版日期:2004-07-26 发布日期:2020-11-20
通讯作者: 毕会民,男,硕士生导师,主任医师,研究方向:Ⅱ型糖尿病的发病机理。Tel:027-62529076 E-mail:bihuimin666@sohu.com
作者简介:李建辉,男,硕士研究生,研究方向:Ⅱ型糖尿病及并发症。
基金资助:
湖北省自然科学基金资助课题(№2003ABA182)

Effects of puerarin on protein expression of protein kinase B in adipose tissue of rats fed by high-fat-diet

LI Jian-Hui, BI Hui-Min, GAN Pei-Zhen, WANG Bao-Hua¹

Department of Endocrine, the People's Hospital of Wuhan University, Wuhan 430060, Hubei, China;
¹Department of Nerve, First People's Hospital of Jingmen, Jingmen 448000, Hubei, China

Received:2004-06-09 Revised:2004-06-26 Online:2004-07-26 Published:2020-11-20

摘要/Abstract

摘要： 目的: 在高脂饮食诱导胰岛素抵抗的基础上,观察葛根素对胰岛素抵抗大鼠脂肪组织中蛋白激酶B 蛋白(PKB)表达的影响。方法: 选取雄性Wistar 大鼠30 只,随机分为正常对照组10只,给予基础饲料;模型组20只,给予高脂饲料。模型组大鼠给予高脂喂养4周后,随机分为2 组:胰岛素抵抗组,继续高脂饮食;葛根素治疗组,继续高脂饮食同时,腹腔注射葛根素注射液(100mg·kg^-1·d^-1)。干预6 周后,蛋白印迹法检测大鼠脂肪组织中胰岛素刺激PKB 的蛋白表达含量。结果: 4周后,高脂喂养鼠的体重、空腹血糖、胰岛素、甘油三脂、胆固醇及胰岛素抵抗指数(HOMA-IR)明显升高,胰岛素敏感指数(ISI)显著下降,出现了胰岛素抵抗。葛根素治疗6周,大鼠的血糖、胰岛素及HOMA-IR下降,ISI显著升高。胰岛素抵抗组大鼠脂肪组织中PKB 的表达明显减少,较对照组下降了23.5 %(P<0.01)。葛根素治疗6周后,大鼠PKB 蛋白表达明显升高,较胰岛素抵抗组增加了18.7%(P<0.01)。结论: 葛根素明显改善胰岛素抵抗,可能与增加脂肪组织中PKB 蛋白表达有关。

关键词: 葛根素, 胰岛素抵抗, 脂肪组织, 蛋白激酶B, 表达

Abstract: AIM: To study the effects of puerarin on protein expression of protein kinase B (PKB) in adipose tissue of rats fed by high-fat-diet.METHODS: 30 male Wistar rats were randomly divided into the control group(n=10) given basic diet, and the model group (n=20) given fat-rich-diet.After 4 weeks, the model group was randomly divided into 2 subgroups :(1) insulin resistant group was continually given high fat-rich-diet ;(2) puerarin-treated group accepted high-fat diet and abdominal injection with puerarin injection (100 mg·kg^-1·d^-1).After 6 week- treatment, the protein expression of PKB stimulated by insulin in adipose tissue was determined with Western blotting at the end of the experiment.RESULTS: The model group showed hyperglycemia, hyperinsulinism, hypertriglyceride, hypercholesterolemia and elevated HOMA insulin resistance index (HOMAIR), while the insulin resistance index (ISI) was obviously reduced compared to the control group, indicating that the insulin resistant model was induced in this way.Puerarin treatment decreased fasting blood glucose and insulin, which was accompanied by lower HOMA-IR and elevated ISI.Because of long term high fat diet, expression of PKB decreased 23.5 %in adipose tissue of the insulin resistant group(P<0.01) compare with the control group.There was an increase of 18.7 %(P<0.01) in PKB expression of the puerarin-treated group compared to the insulin resistant group.CONCLUSION: Puerarin treatment can significantly elevate expression of PKB and ameliorated the stated of insulin resistance.

Key words: puerarin, insulin resistance, adipose tissue, PKB, expression

中图分类号:

R285.

李建辉, 毕会民, 甘佩珍, 汪保华. 葛根素对胰岛素抵抗大鼠脂肪组织中蛋白激酶B 表达的影响[J]. 中国临床药理学与治疗学, 2004, 9(7): 770-773.

LI Jian-Hui, BI Hui-Min, GAN Pei-Zhen, WANG Bao-Hua. Effects of puerarin on protein expression of protein kinase B in adipose tissue of rats fed by high-fat-diet[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(7): 770-773.

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