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中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (7): 815-819.

• 研究原著 • 上一篇    下一篇

纳洛酮干预对大鼠脑出血后血肿周围神经细胞中氧化还原因子-1 表达与凋亡的影响

陈胜会, 孟庆伟, 吴家幂, 储照虎, 张帆1, 徐国祥1   

  1. 皖南医学院弋矶山医院神经内科,1病理科, 芜湖241001, 安徽
  • 收稿日期:2004-03-27 修回日期:2004-06-04 出版日期:2004-07-26 发布日期:2020-11-20
  • 通讯作者: 吴家幂,男,硕士,教授,主任医师,硕士研究生导师,主要从事脑血管病基础和临床研究。
  • 作者简介:陈胜会,男,硕士研究生,讲师,主要从事脑血管病基础和临床研究。Tel:0553-5739314 E-mail:bqtchen@hotmail.com
  • 基金资助:
    安徽省教委自然科学基金(№2003Kj303)

Effects of naloxone on expression of Ref-1 and neuronal apoptosis after intracerebral hemorrhage in rats

CHEN Sheng-Hui, MENG Qing-Wei, WU Jia-Mi, CHU Zhao-Hu, ZHANG Fan, XU Gou-Xiang   

  1. Department of Neurology, Yijishan Hospital, Wannan Medical College, Wuhu 241001, Anhui, China
  • Received:2004-03-27 Revised:2004-06-04 Online:2004-07-26 Published:2020-11-20

摘要: 目的: 观察盐酸纳洛酮对脑出血后血肿周围神经细胞中氧化还原因子-1 (redox factor-1 Ref-1)表达的影响。方法: 应用立体定向技术,将SD 大鼠自体不凝血50 μl 注入其尾状核区制备脑出血模型,将动物随机分为正常对照组,假手术组、出血组和纳洛酮干预组,并分别在不同时间点断头取脑,连续切片作Ref-1 和TUNEL (terminal deoxynucleotidyl transferase[ TdT ]-mediated deoxyuridine triphosphate[ dUTP]-biotin nick end labeling)免疫组化染色。结果: 经盐酸纳洛酮干预后,血肿周围神经细胞中Ref-1 表达与脑出血相对应组比较,在12 h 影响不明显,48 h 能增加Ref-1 表达(P<0.01);72 h 亦能增加Ref-1 表达(P<0.05);盐酸纳洛酮干预性治疗后,血肿周围神经细胞中TUNEL 阳性细胞数与脑出血相对应组比较,在12 h 明显影响,48 h 能明显减少凋亡(P<0.05);72 h 更明显(P<0.01)。结论: 盐酸纳洛酮能通过提高ICH 缺血半暗带区Ref-1 表达等途径,增加修复氧化损伤的DNA 能力,减少细胞凋亡,有脑细胞保护作用。

关键词: 脑出血, 氧化还原因子-1, 细胞凋亡, 纳洛酮, 大鼠

Abstract: AIM: To observe the effects of naloxone on expression of Ref-1 and neuronal apoptosis in rat brain tissue around the caudate nucleus (damaged areas) after experimental intracerebral hemorrhage (ICH).METHODS: ICH was induced in rats using stereotactic infusion autologous blood 50 μl into the caudate nucleus.The male animals were randomly divided into normal control group, sham operation group, hemorrhage group and naloxone treatment group.The hemorrhage group and naloxone treatment group were divided into three different time point groups.TUNEL method was used to detect apoptosis,and immunohistochemitry method to detect expression of Ref-1 in cerebral tissues at different times.RESULTS: The quantity of the expression of Ref-1 within 72 h after ICH was significantly ascended (P<0.05) and the quantity of TUNEL-positive cells within 72 h after ICH was significantly reduced (P<0.01) by treatment with naloxone.CONCLUSION: Naloxone can increase the expression of Ref-1 and the ability of modifying DNA damaged by oxidize, and decrease apoptosis after ICH.

Key words: intracerebral hemorrhage, redox factor-

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