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中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (7): 824-827.

• 研究原著 • 上一篇    下一篇

不同剂量卡维地络干预对压力超负荷幼鼠心肌细胞凋亡和凋亡相关基因表达的影响

叶达平, 单守杰1, 陈绍良1, 周凯2, 叶飞1, 张俊杰1   

  1. 上海梅山第二医院心内科, 南京210041, 江苏;
    1南京医科大学附属南京第一医院心内科, 南京210006, 江苏;
    2南京市儿童医院心内科, 南京210041, 江苏
  • 收稿日期:2004-03-04 修回日期:2004-06-04 出版日期:2004-07-26 发布日期:2020-11-20
  • 通讯作者: 叶达平,男,主治医师,研究方向:冠心病诊疗。Tel:025-6624213-829813851500439

Effects of carvedilol at different doses on cardiac myocyte apoptosis and apoptosis-associated gene in rats with pressure overload

YE Da-Ping, SHAN Shou-Jie1, CHEN Shao-Liang1, ZHOU Kai2, YE Fei1, ZHAN Jun-Jie1   

  1. Department of Cardiology, ShanghaiMeishan Second Hospiatl, Nanjing 210041, Jiangsu, China;
    1Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, Jiangsu, China;
    2Department of Cardiology, Nanjing Children Hospital, Nanjing 210041, Jiangsu, China
  • Received:2004-03-04 Revised:2004-06-04 Online:2004-07-26 Published:2020-11-20

摘要: 目的: 探讨不同剂量卡维地络(CAR)对压力超负荷心肌肥厚幼鼠心肌细胞凋亡的影响,以及CAR 治疗充血性心力衰竭(CHF)的作用机制。方法: 建立腹主动脉狭窄所致压力负荷增加心肌肥厚幼鼠模型,术后1 周开始给予小剂量CAR(0.1mg·kg-1·d-1)和大剂量CAR(10mg·kg-1·d-1)干预7 周,观察不同剂量CAR 对幼鼠血流动力学参数、心肌细胞凋亡、Bcl-2 和P53 蛋白水平表达的影响。结果: CAR 可改善心功能指标,降低心肌细胞凋亡指数、上调Bcl-2 蛋白及下调P53 蛋白表达,HCAR 组作用明显。结论: CAR 可有效减少压力超负荷心肌肥厚幼鼠的心肌细胞凋亡,防治CHF,效果以大剂量明显,这可能与其对凋亡相关基因表达影响的差异有关。

关键词: 心力衰竭, 充血性心力衰竭, 卡维地络, 幼鼠, 细胞凋亡, 基因

Abstract: AIM: To study the effects and mechanism of carvedilol (CAR) at different doses on cardiac myocyte apoptosis in rats with myocardial hypertrophy of CHF.METHODS: Using the animal model of CHF, induced by abdominal aortic constriction in male Wistar rats, hemodynamics parameters, cardiac myocyte apoptosis, and the expression of Bcl-2 and P53 were investigated in the untreated experimental group (CHF group) at 1 week after operation and treated experimental groups in which rats were treated with CAR at a lower dose (LCAR group, 0.1mg·kg-1 ·d-1) and at a higher dose (HCAR group, 10mg·kg-1 ·d-1) for 7 weeks since 1 week after operation.The sham-operated rats were as controls (SH group, n=8).RESULTS: Either doses improved heart function and decreased apoptosis index in rats with CHF.The number of myocytes apoptosis and the level of Bcl-2 were significantly higher and P53 was markedly lower in HCAR group than those in LCAR and MCAR groups.CONCLUSION: CAR can effectively decrease myocardiocyte apoptosis, prevent and cure CHF.The effects are dosedependent, associated with changes of the expression of apoptosis-associated gene.

Key words: heart failure, congestive, carvedilol, apoptosis, gene, rats

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