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中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (8): 939-941.

• 研究原著 • 上一篇    下一篇

黄芩苷对庆大霉素致耳蜗螺旋神经节细胞损伤作用的影响

戴德   

  1. 广东医学院附属医院耳鼻咽喉科, 湛江524001, 广东
  • 收稿日期:2004-06-07 修回日期:2004-06-30 出版日期:2004-08-26 发布日期:2020-11-20
  • 通讯作者: 戴德,男,硕士,副教授,主要从事耳神经病理学研究。Tel:0759-2387024E-mai:daide@126.com
  • 基金资助:
    广东省中医药局科研面上基金项目(№102087)

Protective effect of baicalin-mediated on mice spinal ganglion cells against gentamicin-induced ototoxicity

DAI De   

  1. Department of Otorhinolaryngology, Affiliated Hospital, Guandong Medical College, Zhanjiang 524001, Guangdong,China
  • Received:2004-06-07 Revised:2004-06-30 Online:2004-08-26 Published:2020-11-20

摘要: 目的: 探讨黄芩苷(baicalin,BA)对庆大霉素(gentamicin,GM)致小鼠耳蜗螺旋神经节(spiral ganglion,SG)细胞毒性的拮抗作用。方法: 用MTT法检测庆大霉素对SG 细胞的毒性作用以及黄芩苷对此作用的影响,用紫外分光光度法分别测定各实验组SG 细胞总超氧化物歧化酶(T-SOD)活性,谷胱甘肽过氧化物酶(GSH-PX)活性以及丙二醛(MDA)含量。结果: 黄芩苷可显著抑制GM 所导致的SG细胞中T-SOD 和GSH-PX 活性降低及MDA 含量升高。结论: 黄芩苷通过清除耳蜗螺旋神经节细胞内氧自由基和抑制脂质过氧化反应,拮抗庆大霉素耳毒性作用。

关键词: 黄芩苷, 庆大霉素, 耳蜗螺旋神经节, 自由基, 脂质过氧化

Abstract: AIM: To study the protective effect of baicalin (BA) against the ototoxicity of gentamicin (GM) of mice spinal ganglion (SG) cells.METHODS: MTT assay was employed to determine the growth inhibition of GM on the SG cells.The activities of superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-PX), and the content of malondialdehyde (MDA) were measured by UV chromatography in SG cells from mice of different groups.RESULTS: Baicalin reversibly limited the GM-induced growth inhibition of SG cells in a dose-dependent manner.BA increased the activity of T-SOD and GM-induced decreased activity of T-SOD and GSH-PX in SG cells.The compound could also reduce MDA level and limite the GM-induced high content of MDA in SG cells.CONCLUSION: Baicalin mediated neuroprotective effect on SG cells is against gentamicin-induced ototoxicity through anti-oxidant.

Key words: baicalin, gentamicin, spinal ganglion, free radical, lipid peroxidation

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