红花提取物预防腹主动脉缩窄大鼠左室肥厚的实验研究

摘要/Abstract

摘要： 目的: 研究红花提取物对腹主动脉缩窄术后大鼠左室心肌肥厚的作用,并探讨其作用机制。方法: 取52只Wistar大鼠,行腹主动脉部分缩窄术制作左室心肌肥厚模型,其中12只大鼠行假手术。术后1周将手术组随机分为模型对照组、卡托普利组(100mg·kg^-1)、红花组(40mg·kg^-1),连续灌胃6周后,麻醉大鼠称量大鼠体重、左室心肌重量,计算左心室重量指数(LVMI);观察组织细胞结构改变;应用ELISA法测量血清MMP-2、MMP-9表达。结果: 腹主动脉缩窄术后大鼠LVMI增加,MMP-2、MMP-9的表达增多,红花组、卡托普利组能显著降低LVMI,并且抑制MMP-2、MMP-9的表达。结论: 红花提取物能有效预防因腹主动脉缩窄所致的左心室壁肥厚和LVMI增加,抑制MMP-2、MMP-9的表达,说明红花抑制左室肥厚的形成可能与之有关。

关键词: 红花提取物, 主动脉缩窄, 心室肥厚, 基质金属蛋白酶

Abstract: AIM: To explore the effect of Carthamus tinctorius extract on left ventricularhypertrophy induced by partly abdominal aortic coarctation operation in Wistarrats and to study the involved mechanism.METHODS: 12 ratswere sham operated when the hypertensive hyper-trophy was inducedwith abdominal aortic coarctation in 40 rats,which were randomly divided into control group,captopril group,Carthamus tinctorius extract grouPone week later.The drugs were given by gastric perfusion once a day for6weeks.The weight of the rat and the left ventricle were measured,cardiac coefficient were calcu-lated and MMP-2,MMP-9 were evaluated by ELISA means.RESULTS: The cardiac coefficient of the control grouPwas significantly increased and the expression of MMP-2,MMP-9wasgreatly enhanced in the control rats.After6weeks treatment with captopril and carthamus tinc-torius extract,cardiac coefficient was significantly attenu-ated as well as the expression of MMP-2,MMP-9 in se-rum respectively.CONCLUSION: Carthamus tinctorius extract can prevent from the remodeling process of ventric-ularmuscles,which might be related with the expressive depression of MMP-2,MMP-9.

Key words: carthamus tinctorius extract, aorta co-arctation, myocardium hypertrophy, matrix metalloprotei-nase

中图分类号:

R282.71

王建萍, 王雄. 红花提取物预防腹主动脉缩窄大鼠左室肥厚的实验研究[J]. 中国临床药理学与治疗学, 2005, 10(11): 1290-1293.

WANG Jian-ping, WANG Xiong. Study of carthamus tinctorius extract on left ventricle in pressure-over-loaded hypertrophy rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(11): 1290-1293.

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