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中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (2): 137-140.

• 研究原著 • 上一篇    下一篇

γ-干扰素对肝纤维化大鼠I、III 型胶原表达的影响

李萍, 胡国龄, 谭德明, 刘国珍, 汪玲   

  1. 中南大学湘雅医院传染病研究所, 长沙410008, 湖南
  • 收稿日期:2004-11-05 修回日期:2005-01-21 出版日期:2005-02-06 发布日期:2020-11-18
  • 通讯作者: 胡国龄, 女, 博士生导师, 研究方向:肝脏纤维化。Tel:13187032573 E-mail:lplxg@yahoo.com.cn
  • 作者简介:李萍, 女, 博士, 研究方向:肝脏纤维化。

Effect of γ-interferon on expression of collagen I, III of hepatic fibrosis in rats

LI Ping, HU Guo-ling, TAN De-ming, LIU Guo-zhen, WANG Ling   

  1. Institute of Infectiou Diseases, Xiangya Hospital, Central South University, Changsha 410008, Hunan, China
  • Received:2004-11-05 Revised:2005-01-21 Online:2005-02-06 Published:2020-11-18

摘要: 目的:探讨γ-干扰素抗肝纤维化的治疗机制。方法:17 只正常雄性Wistar 大鼠分成3 组, 除正常对照组外, 余2 组大鼠肝纤维化造模均采用腹腔注射猪血清(每周2 次, 每次0.5 ml) 。γ-干扰素治疗组在造模第9 周每天皮下注射10 万单位的干扰素。模型组和正常对照组给等量的生理盐水灌胃。12 周处死大鼠, HE 和Masson 染色观察肝纤维化的形成, RT-PCR 检测肝组织中I、III 型胶原mRNA 的表达, 免疫组化法观察I、III 型胶原蛋白的表达。结果:γ-干扰素显著逆转了猪血清诱导的肝纤维化, 与模型组比较, γ-干扰素治疗组能明显降解纤维化大鼠肝中的胶原(P<0.05) 。PT-PCR 分析可见I、III型胶原mRNA 的表达在γ-干扰素治疗组均明显减少。I、III 型胶原蛋白的表达在γ-干扰素治疗组均明显减少。结论:γ-干扰素能逆转免疫引起的肝纤维化, 这是由于其能促进肝脏胶原的降解作用, 最终导致肝纤维化的逆转。

关键词: γ-干扰素, 肝纤维化, I、III型胶原, 肝脏, 大鼠

Abstract: AIM: To further explor and study the mechanism of γ-interferon in treating hepatic fibrosis on the basis of clinical practice of treatment for hepatic fibrosis. METHODS: 17 male Wistar rats were divided into three groups.The rat fibrosis model was induced by pig serum except for normal control group.The other two groups were both given intraperitoneal injection of pig serum respectively (0.5 ml once, 2 times per week, total 12 weeks).In γ-interferon treated group, the rats were subcutaneous injected γ-interferon one hundred thousand IU every day at the ninth week.The model group and normal control group were fed with the same amount of saline by gavage.The rats were killed at the end of the twelfth weeks, the pathology of liver fibrosis was observed with HE stain and Masson stain.Collagen I mRNA and collagen III mRNA were detected in liver samples with RT-PCR, immunohistochemistry was used to observe the expression of collagen I, III protein.RESULTS: Upon pathological examination, the γ-interferon treatment had significantly reversed pig serum-induced liver fibrosis.In γ-interferon treatment group, the γ-interferon could enhance the degradation of collagen in the rat fibrotic liver according to HE and Masson staining compared with the fibrotic model group(P<0.05).Moreover, in γ-interferon treatment group, the expression of collagen I, III and its protein in liver were markedly reduced, respectively according to RT-PCR analysis and immunohistochemistry as compared with model group.CONCLUSION: The rat hepatic fibrosis induced by pig serum can be obviously reversed by γ-interferon.The mechanism of treating hepatic fibrosis with γ-interferon appears to be due to the enhancement of the degradation of collagen in liver, at last resulted in hepatic fibrosis been reversed eventually.

Key words: hepatic fibrosis, collagen I,III, γ-interferon, liver, rats

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