胆碱对小鼠炎性疼痛影响的实验研究

中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (3): 276-280.

胆碱对小鼠炎性疼痛影响的实验研究

苏冬梅^1,2, 刘跃², 王越^1,3, 王汝欢¹, 汪海²

¹北京赛德维康医药研究院, 北京100850;
²军事医学科学院毒物药物研究所, 北京100850;
³山东大学医学院解剖教研室, 济南250012, 山东

收稿日期:2004-12-22 修回日期:2005-01-27 出版日期:2005-03-26 发布日期:2020-11-18
通讯作者: 汪海, 男, 教授, 博士生导师, 研究方向:心血管药理和新药研究工作。Tel:010-66932651 E-mail:wh@nic.bmi.ac.cn
作者简介:苏冬梅, 女, 医师, 硕士, 研究方向:神经药理学。
基金资助:
国家自然科学基金资助项目(No30371641);北京赛德维康医药研究院新药研究基金项目资助(No1999001)

Antinociceptive effect of choline on inflammatory pain in mice

SU Dong-mei^1,2, LIOU Yue², WANG Yue^1,3, WANG Ru-huan¹, WANG Hai²

¹Thadweik Academy of Medicine, Beijing 100850, China;
²Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China;
³School of Medicine, Shandong University, Jinan 250012, Shandong, China

Received:2004-12-22 Revised:2005-01-27 Online:2005-03-26 Published:2020-11-18

摘要/Abstract

摘要： 目的:研究胆碱对炎性疼痛的影响。方法:福尔马林实验和小鼠热板实验, 观察单独给予胆碱及胆碱与阿司匹林或吗啡联合用药时, 对小鼠在福尔马林模型中的舔足时间和热板实验中的疼痛潜伏期的影响。结果:胆碱(4、8、16、32、64 mg°kg^-1, i.v.)可显著抑制福尔马林所致的Ⅱ相痛反应;尾静脉给予胆碱2 mg°kg^-1 或阿司匹林9.4 mg°kg^-1, 对福尔马林模型的Ⅰ 相和Ⅱ相痛反应均无显著的抑制作用, 二者联合应用可显著抑制Ⅱ相反应;联合给予吗啡0.165 mg°kg^-1 和胆碱2 mg°kg^-1对福尔马林模型的Ⅱ 相反应的抑制作用比单独应用吗啡0.165mg°kg^-1好。结论:胆碱对炎症性疼痛镇痛效果显著, 胆碱和阿司匹林、吗啡有协同镇痛作用。

关键词: N受体激动剂, 胆碱, 镇痛, 协同作用, 福尔马林实验

Abstract: AIM: To investigate the antinociceptive effect of choline on inflammatory pain.METHODS: Formalin and hot-plate tests were used to study the antinociceptive effect of choline, and observe the synergetic antinociceptive effects of choline and its combination with aspirin or morpine.RESULTS: Early phase (0-5 min) response was induced by an immediate and intense increase in the spontaneous activity of afferent C fibers. Late phase (15-30 min) response was thought to be inflammatory response induced by the release of inflammatory factors after formalin administration.Choline (4, 8, 16, 32, and 64mg°kg^-1, iv) obviously shortened the paw licking time in phase Ⅱ in the formalin test.The conjoint administration of choline 2 mg°kg^-1 and aspirin 9.4 mg°kg^-1 produced a synergistic antinociception effect compared with that administration of choline 2 mg°kg^-1 or aspirin 9.4 mg°kg^-1, respectively.And the phenomenon was also observed on the conjoint administration of choline 2 mg°kg^-1 and morpine 0.165 mg°kg^-1 compared with that administration of morpine 0.165 mg°kg^-1 singly, but synergistic effect on the conjoint administration of choline 4 mg°kg^-1 and aspirin 23.5 mg°kg^-1 did not show obvious significance compared with that administration of choline 4 mg°kg^-1 or aspirin 23.5 mg°kg^-1, respectively.CONCLUTION: Choline can inhibit inflammatory pain induced by formalin.Co-administrations of choline and aspirin, or morpine have prominent synergetic antinociceptive effects.

Key words: nAchR agonist, choline, synergistic antinociception, formalintest

中图分类号:

R971.91

苏冬梅, 刘跃, 王越, 王汝欢, 汪海. 胆碱对小鼠炎性疼痛影响的实验研究[J]. 中国临床药理学与治疗学, 2005, 10(3): 276-280.

SU Dong-mei, LIOU Yue, WANG Yue, WANG Ru-huan, WANG Hai. Antinociceptive effect of choline on inflammatory pain in mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(3): 276-280.

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