3,4,5,6-四羟基口山酮对高糖所致血管内皮依赖性舒张功能减退的保护作用

中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (3): 333-337.

3,4,5,6-四羟基_口山酮对高糖所致血管内皮依赖性舒张功能减退的保护作用

戴忠, 姜德建¹, 戴滨, 鲁澄宇, 许卫铭

广东医学院药理教研室, 湛江524023, 广东;
¹中南大学药学院药理学系, 长沙410078, 湖南

收稿日期:2004-12-15 修回日期:2005-01-25 出版日期:2005-03-26 发布日期:2020-11-18
通讯作者: 戴忠, 男, 医学博士, 副教授, 研究方向:心血管药理学。Tel:0759-2388588-3 E-mail:daizhong dai@yahoo.com.cn

Protective effects of 3,4,5,6-tetrahydroxanthone on damages of endothelium-dependent relaxation induced by high glucose

DAI Zhong, JIANG De-jian¹, DAI Bin, LU Cheng-yu, XU Wei-ming

Department of Pharmacology, Guangdong Medical University, Zhanjiang 524023, Guangdong, China;
¹Department of Pharmacology, School of Pharmaceutical Science, Central South University, Changsha 410078, Hunan, China

Received:2004-12-15 Revised:2005-01-25 Online:2005-03-26 Published:2020-11-18

摘要/Abstract

摘要： 目的:观察3, 4, 5, 6-四羟基_口山酮对高糖所致血管内皮依赖性舒张功能减退的保护作用。方法:在大鼠离体胸主动脉环, 用高浓度葡萄糖(25mmol°L^-1) 孵育24 h, 检测乙酰胆碱诱导的血管内皮依赖性舒张反应;人脐静脉内皮细胞株(ECV304)用高浓度葡萄糖(30 mmol°L^-1) 培养48 h, 测定培养液中乳酸脱氢酶(LDH) 活性、一氧化氮(NO) 及细胞脂质过氧化物丙二醛(MDA) 含量。结果:3, 4, 5, 6-四羟基_口山酮(1、3 和10 μmol°L^-1) 能显著抑制高糖所致的舒血管效应减退, 并且呈剂量依赖性。3, 4, 5,6-四羟基_口山酮(1、3 和10 μmol°L^-1) 能显著抑制高糖所致的内皮细胞LDH 泄漏、NO 释放和MDA 生成的增加。结论:3, 4, 5, 6-四羟基_口山酮对高糖所致的血管内皮依赖性舒张功能减退有保护作用, 其保护作用与抑制脂质过氧化减少血管内皮细胞损伤有关。

关键词: 3,4,5,6-四羟基_口山酮, 高糖, 内皮, 脂质过氧化

Abstract: AIM: To investigate the protective effects of 3, 4, 5, 6-tetrahydroxanthone on damages of endothelium- dependent relaxation induced by high glucose. METHODS: The aortic rings obtained from the rats were exposed to high glucose (25 mmol°L^-1) for 24 h or endothelial cells were incubated with high glucose (30 mmol°L^-1) for 48 h, respectively.Endothelium-dependent relaxation was measured.Activity of lactate dehydrogenase (LDH), contents of malondialdehyde (MDA) and nitric oxide (NO) in the medium were measured.RESULTS: Exposure of aorta rings to high glucose (25 mmol°L^-1) for 24 h caused a marked decrease in endotheliun- dependent relaxation.Pretreatment with 3, 4, 5, 6- tetrahydroxyxanthone (1, 3 or 10 μmol°L^-1) or probucol (10 μmol°L^-1) significantly improved endothelium-dependent relaxation in a dose-dependent manner.The release of LDH, the contents of MDA and NO in the medium marked increased after exposure of HUVEC to high glucose (30mmol°L^-1) for 48 h.Pretreatment with 3, 4, 5, 6-tetrahydroxyxanthone (1, 3 or 10 μmol°L^-1) or probucol (10 μmol°L^-1) significantly decreased the increased release of LDH, the increased content of NO and MDA in the medium.CONCLUSION: 3, 4, 5, 6-tetrahydroxanthone may preserve endothelial dysfunction induced by high glucose, and the protective effects of 3, 4, 5, 6-tetrahydroxanthone may be related to reduction of endothelial cells damages by inhibiting lipid peroxidation.

Key words: 3,4,5,6-tetrahydroxanthone, high glucose, endothelium, lipid peroxidation

中图分类号:

R966

戴忠, 姜德建, 戴滨, 鲁澄宇, 许卫铭. 3,4,5,6-四羟基_口山酮对高糖所致血管内皮依赖性舒张功能减退的保护作用[J]. 中国临床药理学与治疗学, 2005, 10(3): 333-337.

DAI Zhong, JIANG De-jian, DAI Bin, LU Cheng-yu, XU Wei-ming. Protective effects of 3,4,5,6-tetrahydroxanthone on damages of endothelium-dependent relaxation induced by high glucose[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(3): 333-337.

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