雌激素对老年雌性去势大鼠肝脏代谢能力及抗氧化功能的影响

中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (4): 424-427.

雌激素对老年雌性去势大鼠肝脏代谢能力及抗氧化功能的影响

张韶英, 平洁, 汪晖

武汉大学医学院药理学系, 武汉 430071,湖北

收稿日期:2005-02-08 修回日期:2005-04-09 出版日期:2005-04-26 发布日期:2020-11-19
通讯作者: 汪晖,女, 教授, 博士生导师, 主要从事生化药理与药物代谢研究。Tel:027-87331565 E-mail:clbwhcbd@yahoo.com
作者简介:张韶英, 女, 硕士研究生, 现在武汉市第二医院老年科, 主要从事老年学病研究。Tel:027-62566191

Influence of estrogen on metabolism and antioxidative functions in old cas-trated female rats

ZHANG Shao-ying, PING Jie, WANG Hui

Department of Pharmacology, Medical College of Wuhan University, Wuhan 430071, Hubei, China

Received:2005-02-08 Revised:2005-04-09 Online:2005-04-26 Published:2020-11-19

摘要/Abstract

摘要： 目的: 观察老年雌性去势大鼠补充乙烯雌酚后肝脏药物代谢酶活性及抗氧化功能的变化, 并探讨更年期应用雌激素的生理学和药理学意义。方法: 切除大鼠双侧卵巢造成雌激素锐减。术后 d3经口灌胃乙烯雌酚(100 μg°kg^-1, qd×40 d),每天 1次,连续 40 d。观察大鼠肝脏药物代谢酶活性和抗氧化指标的变化。结果: 去势大鼠肝微粒体细胞色素P450及细胞色素 b₅ 含量显著降低, NADPH-细胞色素 C 还原酶、苄非他明 N-脱甲基酶、氨基比林 N-脱甲基酶活性也明显降低。肝微粒体及肝匀浆中谷胱甘肽 S-转移酶活性显著降低, 肝匀浆谷胱甘肽含量减少, 脂质过氧化产物丙二醛增加。给予乙烯雌酚后, 除对细胞色素 b₅ 无明显影响外, 上述指标均可逆转。结论: 老年雌性大鼠去势后肝脏代谢能力及抗氧化功能的降低与雌激素水平低下有关。乙烯雌酚能有效地改善上述变化, 维持肝脏正常的代谢能力及抗氧化功能。

关键词: 去势, 大鼠, 雌激素, 药物代谢功能, 抗氧化功能

Abstract: AIM: To investigate the influence of di-ethylstilbestrol on metabolism and antioxidative functions in old castrated female rats in order to elucidate the physi-ological and pharmacological effects of estrogen therapy in climacteric. METHODS: The old female rats had been castrated, liver drug-metabolizing enzyme activities and antioxidative indexes were measured in rats treated with andwithout diethylstilbestrol (100 μg°4kg^-1, qd×40 d, ig). RESULTS: The activities of microsomal NADPH-Cyt C reductase, benzphetamine N-demethylase, aminopyrine N-demethylase, glutathione S-transferase, and the contents of microsomal cytochrome P450, cy-tochrome b₅ and homogenate glutathione remarkable de-creased in the liver of castrated female rats. Meanwhile, an increase of liver malondialdehyde content was also ob-served. Treatment with diethylstilbestrol could reverse the changes of above indices except for cytochrome b₅. CONCLUSION: The decreased metabolism and antioxidative functions are related to the lower level of estrogen in old castrated female rats.Estrogen plays a significant role in keeping normal metabolism and antioxidative capacities of the liver.

Key words: castrated, rats, estrogen, drug-metabo-lizing function, antioxidative function

中图分类号:

R977.12

张韶英, 平洁, 汪晖. 雌激素对老年雌性去势大鼠肝脏代谢能力及抗氧化功能的影响[J]. 中国临床药理学与治疗学, 2005, 10(4): 424-427.

ZHANG Shao-ying, PING Jie, WANG Hui. Influence of estrogen on metabolism and antioxidative functions in old cas-trated female rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(4): 424-427.

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