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中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (4): 443-446.

• 研究原著 • 上一篇    下一篇

大豆异黄酮活性组分对 H2O2 诱导的 PC12 细胞损伤的影响

陈伟强, 赵宇红1, 罗少洪2, 杨红2, 金桂芳2, 潘伟2   

  1. 广东药学院病理生理教研室,1药理教研室, 2生物化学教研室, 广州 510224, 广东
  • 收稿日期:2005-01-25 修回日期:2005-03-22 出版日期:2005-04-26 发布日期:2020-11-19
  • 通讯作者: 杨红,女, 副教授, 硕士生导师, 研究方向:生化技术与生物制药研究。Tel:020-9352067  E-mail:yanghong @gdpc.edu.cn
  • 作者简介:陈伟强, 男, 实验师, 研究方向:中西医结合病理生理研究。Tel:(0) 13076785931 E-mail:cwq66712@126.com
  • 基金资助:
    广东省科技厅计划项目(NO2004B30101001); 广东省中医药局科研项目(NO103079)

Effects of active ingredient of soybean isoflavoneson on PC12 cell injuries induced by H2O2

CHEN Wei-qiang, ZHAO Yu-hong1, LUO Shao-hong2, YANG Hong2, JING Gui-fang2, PAN Wei2   

  1. Department of Pathophysiology,1Department of Pharmacology,2Department of Biochemistry, Guangdong College of Phar-macy, Guangzhou 510224, Guangdong, China
  • Received:2005-01-25 Revised:2005-03-22 Online:2005-04-26 Published:2020-11-19

摘要: 目的: 探讨大豆异黄酮活性组分对 H2O2 诱导的PC12细胞损伤的保护作用。方法: 以 H2O2 损伤PC12细胞为氧化应激损伤的模型, 采用光学显微镜观察细胞形态, MTT 法判断细胞的存活率, LDH 法检测细胞的损伤程度。结果: 不同浓度大豆异黄酮能明显改善 H2O2 导致的细胞甲瓒减少, 大豆异黄酮处理组细胞存活率明显高于 H2O2 处理组(P<0.01);而 LDH 漏出率则明显低于H2O2 处理组(P<0.05)。结论: 大豆异黄酮活性组分对 H2O2 诱导的PC12细胞损伤具有保护作用。

关键词: H2O2, PC12, 大豆异黄酮, 细胞损伤, 阿尔茨海默病

Abstract: AIM: To explore the effects of active in-gredient of soybean isoflavones on PC12 cells injuries in-duced by H2O2. METHODS: Active ingredients of soy-bean were extracted and purified.PC12 cells cultured in vitro was pretreated by different dosage of the ingredients about 12 hours and then injured by H2O2 (10 mmol°L-1). Cell viability rate in all groups was tested by MTT assay. Activity of LDH of all groupswas tested. RESULTS: The viability rates in all groups pretreated was significantly increased compared with only H2O2 treat-ment group (P<0.05). The activity of LDH in all pre-treatment group was significantly decreased (P<0.05) compared with H2O2 treatment group.CONCLUSION: Active ingredient of soybean isoflavoneson can restrain cy-totoxicity induced by H2O2 and protect cells from peroxy-dation.

Key words: H2O2, PC12, soybean isoflavones, cell injury, Alzheimers disease

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