欢迎访问《中国临床药理学与治疗学》杂志官方网站,今天是

中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (4): 447-450.

• 研究原著 • 上一篇    下一篇

正交实验建立蛇毒抗瘤蛋白生物活性测定方法

乐嘉静, 李湛君, 徐康森   

  1. 中国药品生物制品检定所生化药品室, 北京 100050
  • 收稿日期:2004-09-25 修回日期:2004-11-16 出版日期:2005-04-26 发布日期:2020-11-19
  • 通讯作者: 乐嘉静,女, 硕士研究生, 副主任药师, 主要从事新药药效的评价。Tel:013911049201 E-mail:lejiajing@shou.com

Determination of biological activity of atranticance by orthogonal test

LE Jia-jing, LI Zhan-jun, XU Kang-sen   

  1. National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China
  • Received:2004-09-25 Revised:2004-11-16 Online:2005-04-26 Published:2020-11-19

摘要: 目的: 建立蛇毒抗瘤蛋白生物活性测定方法。方法: 用正交实验确定实验条件, 求细胞 50%死亡时的样品浓度, 即 IC 50 值。结果: 此法使细胞死亡率与样品 的 Log 浓 度具 有良好 的线 性关 系 (r =0.9515, P <0.01), 重复性实验结果:板内 RSD 值为12.3%, 板间 RSD 值为 16.6%;不同实验次数 RSD值为 17.8%, 灵敏度为 36 μg°L-1, 并能反应出蛇毒抗瘤蛋白的抗肿瘤特异性。结论: 此方法特异, 简便且具有良好的重复性, 可以作为蛇毒抗瘤蛋白生物活性的测定方法。

关键词: 蛇毒抗瘤蛋白, 生物活性, 正交实验, MTT, K562细胞株

Abstract: AIM: To develop a method for determi-nation of the biological activity of atranticance. METHODS: The conditions of the experiment were determined by orthogonal test, then IC 50 which was the concentration of the sample when the 50% death of cells was tested. RESULTS: The mortality rate had a good correlativity with Log consistency of the sample, (r =0.9515, P<0.01). And the coefficients of variation of reproducibility of intra-, inter and different test assays were 12.3%, 16.6% and 17. 8%, respectively. CONCLUSION: Thismethod is superfine, simple and highly reproducible. It can be used for determining the biological activity of a-tranticance.

Key words: atranticance, activity, orthogonal test, MTT, K562

中图分类号: