欢迎访问《中国临床药理学与治疗学》杂志官方网站,今天是

中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (5): 574-578.

• 研究原著 • 上一篇    下一篇

阿司匹林-烟酰胺-锌络合物对血小板聚集作用和实验性血栓形成的影响

蒲其松, 雷军, 张翔   

  1. 川北医学院药理教研室, 南充 637000, 四川
  • 收稿日期:2005-03-07 修回日期:2005-04-27 出版日期:2005-05-26 发布日期:2020-11-19
  • 通讯作者: 蒲其松,男, 副教授, 主要从事水杨酸类解热镇痛药的研究与开发。Tel:0817-2242761 E-mail: puqisong@yahoo.com. cn
  • 基金资助:
    国家重点科技攻关项目(No96-901-05-61)

Effects of aspirin-niacinamide-zinc complex (WUY) on platelet aggrega-tion and experimental thrombosis

PU Qi-song, LEI Jun, HANG Xiang   

  1. Department of Pharmacology, North Sichuan Medical College, Nanchong637000, Sichuan, China
  • Received:2005-03-07 Revised:2005-04-27 Online:2005-05-26 Published:2020-11-19

摘要: 目的: 对阿司匹林-烟酰胺-锌络合物(WUY)的抗血小板聚集作用和抗实验性血栓形成作用进行研究。方法: 用 Born 法测定 WUY 对抗腺苷二磷酸钠(ADP)、花生四烯酸(AA) 和胶原诱导大鼠体外或体内血小板最大聚集, 用小鼠静注 AA 引起的死亡率以及大鼠实验性血栓形成研究药物的抗血栓作用, 并且用放免法测定家兔口服药物后血小板释放产物血栓烷 B2 (TXB2) 和 6-酮前列腺素 F (6-keto-PGF) 的含量。结果: WUY 高、中、低 3 种剂量在体外都能显著对抗诱导剂(ADP、AA 和胶原) 引起的血小板聚集, 其中高剂量组的抗血小板聚集作用比阿司匹林(ASP) 强。在体内, WUY 明显抑制 AA 诱导的血小板聚集作用, 1 h 和 3 h 作用最强。WUY 对小鼠尾静脉注射 AA 后诱发的肺栓塞死亡有较强的预防作用, ED50 比ASP 低, 对大鼠实验性血栓形成也有显著的抑制作用。WUY 高剂量能显著抑制血浆中TXB2 水平和提高血浆中6-keto-PGF 含量, 而ASP则降低血浆中 TXB2 和 6-keto-PGF 的水平。结论: WUY 具有比ASP 更强的抗血小板聚集作用和抗实验性血栓形成作用, 并能提高血浆中 6-keto-PGF 1α 含量。

关键词: 阿司匹林-烟酰胺-锌络合物, 阿司匹林, 血小板聚集, 血栓形成, 血栓烷 B2, 6-酮前列腺素 F

Abstract: AIM: To study the effects of aspirin-ni-acinamide-zinc complex (WUY) on platelet aggregation and experimental thrombosis.METHODS: With ade-nosine diphosphatethe (ADP), arachidonic acid (AA) and collagen, the effectsof aspirin-niacinamide-zinc com-plex (WUY) on platelet aggregation in vitro or in vivo were investigated by Born'smethod.The mouse mortality caused by intravenous injection of AA and experimental thrombus formation in rats were observed.Radioimmuno-assay was used for measuring thromboxane B2 (TXB2) and 6-keto-PGFin plasma of rabbits.RESULTS: In high, middle and low dose groups, drugs, in vitro, inhibited ADP-, AA-and collagen-induced platelet aggregation and the effect of WUY was stronger than that of ASP in high dose groups.In vivo, WUY showed more potent inhibito-ry effects on AA-induced aggregation in 1 h and 3 h. WUY had a powerful inhibitory effect on mouse death as a result of pulmonary thrombi induced by AA injection into the tail vein and ED50 was lower than that of ASP. In ad-dition, WUY exhibited strong inhibitory effect on throm-bus formation in rat arteri-venous shunt and significantly reduced plasma level of TXB2 while it markedly increasing 6-keto-PGFin high dose groups and ASP significantly reduced plasma level of both TXB2 and 6-keto-PGF. CONCLUSION: The effect of WUY on platelet aggrega-tion and experimental thrombosis is stronger than that of ASP and can increase plasma level of 6-keto-PGF.

Key words: aspirin-niacinamide-zinc complex, aspi-rin, platelet aggregation, thrombosis, TXB2, 6-keto-PGF

中图分类号: