复方心康口服液心肌保护作用的实验研究

中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (5): 579-585.

复方心康口服液心肌保护作用的实验研究

吴红玲, 傅颖君¹, 王芳, 庞红, 苏卓娃², 何明¹

深圳市第六人民医院心内科, ¹江西医学院药理教研组, 南昌 330006, 江西;
²中心实验室, 深圳 518052, 广东

收稿日期:2004-12-28 修回日期:2005-04-23 出版日期:2005-05-26 发布日期:2020-11-19
通讯作者: 吴红玲,女, 主任医师, 研究方向:心血管疾病的防治。Tel:( 0) 13922822579 E-mail:wuhl45@yahoo.com
基金资助:
深圳市科技计划项目(No200204149)

Experimental study of protective effects of compound oral liquid xinkang on myocardium

WU Hong-ling, FU Ying-jun¹, WANG Fang, PANG Hong, SU Zhuo-wa², HE Ming¹

Department of Cardiology,¹Department of Pharmacology, Jiangxi Medical College, Nanchang 330006, Jiangxi, China;
²Central Laboratory,Shenzhen Sixth People's Hospital, Shenzhen 518052, Guangdong,China

Received:2004-12-28 Revised:2005-04-23 Online:2005-05-26 Published:2020-11-19

摘要/Abstract

摘要： 目的: 探讨心康口服液对急性心肌损伤的保护作用。方法: (1) 将小鼠分为对照组、运动衰竭组(运衰组)、牛磺酸+运衰组(牛磺酸组)、丹参 +运衰组(丹参组), 采用不同比例配伍的心康口服液用于小鼠, 观察其游泳至衰竭时间。(2) 用 Wistar 大鼠建立离体心脏模型及心脏损伤模型, 分为对照组、缺血损伤(A R) 组、心康组, 分别观察各组大鼠心功能指标、CPK 活性、心肌组织 MDA 及 GSH-PX, SOD 活性、心肌细胞超微结构。(3) 以 1 ~ 3 d 的 SD 大鼠的心室肌细胞为基质, 建立心肌细胞缺氧/复氧损伤模型, 分为对照组(复氧组)、A R(缺氧/复氧) 组、心康组, 观察 3 组的心肌细胞存活率、培养液中 LDH 活性、心肌细胞超微结构。结果: (1) 牛磺酸组、丹参组较衰竭组小鼠的游泳时间显著延长(P<0.05), 血清中 CPK 活力低于运衰组(P<0.05)。心康口服液中牛磺酸对小鼠的游泳能力影响较大, 且高浓度好;丹参的影响次之, 中浓度较好。(2) 对照组整个灌流期间 LVSP、dp/dt_max、HR均无明显变化, 心肌细胞超微结构正常。A/R 组在复灌 5、10、20、30 min 时LVSP、dp/dt max、HR 下降(P<0.01), 心肌酶活力降低,MDA 含量及 CPK 活性升高(P<0.01), 心肌细胞超微结构破坏, 成不可逆损伤, 心康组在复灌 5、10、20、30 min 时 LVSP、dp/dt_max、HR 与 A/R 组相比均升高(P<0.01);心肌酶活力均升高, MDA 含量及CPK 活性降低(P<0.01);细胞超微结构大有改善。(3)A R 组与对照组相比其细胞存活率降低(P<0.01), LDH 活力升高(P<0.01), 心康组与 A R 组相比细胞存活率升高(P<0.01);LDH 活力降低(P<0.01);对照组心肌细胞超微结构正常, A/R 组心肌细胞超微结构破坏, 成不可逆损伤, 心康组细胞超微结构大有改善。结论: 牛磺酸和丹参配物而成的心康口服液, 对心肌缺血/再灌注损伤具有显著的保护作用, 心康口服液作为心肌缺血/再灌注损伤治疗的辅助性用药将具有较好的临床应用前景。

关键词: 牛磺酸, 丹参, 心肌, 缺氧/复氧, 大鼠

Abstract: AIM: To explore the protective effects of compound oral liquid xinkang on the acute myocardial in-jury. METHODS: The rats were divided into four groups:control group, sport group, Tau group and Ts group.9 groups of rats were taking compound oral liquid xinkang of different ingredient, and the swimming time was observed.Exosomatic heart model of Wistar ratswere divided into three groups:control group, A/R group and xinkang group. Heart function index, CPK activity, MDA, GSH-PX, SOD and myocardial microscopic struc-ture were observed.The ventricular myocardial cells of 1 -3 days old SD rats were divided into three groups.In control group, the cells were cultivated with reperfusion liquid.In A/R group, the cells were cultivated with an-oxia/reperfusion liquid.In xinkang group, the cells were first cultivated with xinkang oral liquid and second with anoxia/reperfusion liquid. Myocardial survival ratio, LDH activity in cultivating liquid and myocardial microscopic structure were observed. RESULTS: Swimming time in Tau and Ts groupwere longer than that in sport group (P<0.05). Serum CPK in Tau and Ts group were lower than that in sport group (P<0.05). Taurine influenced the swimming time much more than salvia miltiorrhiza. Taurine of high concentration and salvia miltiorrhiza of middle concentration were better. LVSP, dp/dt max and HR in control group were steady during perfusion, but those in A Rgroupwere decreased during perfusionfor 5, 10, 20 and 30 min (P<0.01), and those in xinkang group were higher than those in control group during per-fusion for 5, 10, 20 and 30 min (P<0.01). In A/R group, myocardial enzyme activity was lower, and MDA and CPK were higher than those in control group (P< 0.01). The results were inverse contrast xinkang group with A R group.Myocardial microscopic structure in con-trol groupwas normal, and that in A R group was serious-ly destroyed. The structure in xinkang group was im-proved. Myocardial survival ratio in A/R group was lower than that in control group (P<0.01). The ratio in xin-kang group was higher than that in A/R group (P< 0.01). LDH activity in A/R group was higher than that in control group (P<0.01). The activity in xinkang group was lower than that in A/R group (P<0.01). Myocardial microscopic structure in control groupwas nor-mal, and that in A/R group was seriously destroyed.The structure in xinkang group was improved.CONCLUSION: Oral liquid xinkang compounded of taurine and salvia miltiorrhiza can protect the myocardium experienced anoxia/reperfusion.

Key words: taurine, salvia miltiorrhiza, myocardi-um, anoxia/reperfusion, rats

中图分类号:

R972

吴红玲, 傅颖君, 王芳, 庞红, 苏卓娃, 何明. 复方心康口服液心肌保护作用的实验研究[J]. 中国临床药理学与治疗学, 2005, 10(5): 579-585.

WU Hong-ling, FU Ying-jun, WANG Fang, PANG Hong, SU Zhuo-wa, HE Ming. Experimental study of protective effects of compound oral liquid xinkang on myocardium[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(5): 579-585.

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