一氧化氮部分介导吡格列酮抑制培养兔胸主动脉平滑肌细胞增殖

中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (6): 642-645.

一氧化氮部分介导吡格列酮抑制培养兔胸主动脉平滑肌细胞增殖

陈鹭颖^1,2, 徐江平¹, 庞建新¹, 史道华²

¹南方医科大学药理学教研室, 广州510515, 广东;
²南京军区福州总医院药理科, 福州350025, 福建

收稿日期:2005-04-02 修回日期:2005-05-26 出版日期:2005-06-26 发布日期:2020-11-12
通讯作者: 史道华, 男, 博士, 教授, 硕士生导师, 研究方向:心血管药理学。Tel:0591-83739964 E-mail: shidh@pub2.fz.fj.cn
作者简介:陈鹭颖, 女, 在职硕士研究生, 主管药师。
基金资助:
福建省自然科学基金资助项目(NOC97051)

Antiproliferative effects of pioglitazone involved of nitric oxide on cultured rabbit aortic smooth muscle cells

CHEN Lu-ying^1,2, XU Jiang-ping¹, PANG Jian-xin¹, SHI Dao-hua²

¹Department of Pharmacology, Nanfang Medical University, Guangzhou 510515, Guangdong, China;
²Department of Pharmacology, Fuzhou General Hospital of Nanjing Military Command, Fuzhou 350025, Fujian, China

Received:2005-04-02 Revised:2005-05-26 Online:2005-06-26 Published:2020-11-12

摘要/Abstract

摘要： 目的: 观察吡格列酮(PIO) 对高糖高胰岛素诱导兔胸主动脉平滑肌细胞(VSMCs) 增殖的影响及其与一氧化氮(NO) 的关系。方法: 模拟胰岛素抵抗(IR) 状态培养VSMCs, 四甲基氮唑盐(MTT) 比色法测定细胞增殖, 试剂盒分别检测细胞液中NO 含量及细胞总一氧化氮合酶(NOS) 、诱导型一氧化氮合酶(iNOS) 活性。结果: PIO 干预后, 正常培养组和模拟IR 培养组VSMCs 含MTT 溶解物的吸光值(A₄₉₀)均低于各自对照组(P <0.01) 。PIO 显著增加VSMCs 总NOS 活性、iNOS 活性以及NO 含量(P <0.01) 。PIO 上述作用在模拟IR 培养时更强, 均可被NOS 抑制剂L-NAME 部分阻断。结论: 在正常或模拟IR 培养时, PIO 抑制VSMCs 增殖的作用部分通过NO 介导。

关键词: 一氧化氮, 一氧化氮合酶, 吡格列酮, 血管平滑肌细胞, 胰岛素抵抗

Abstract: AIM: To investigate the antiproliferative effects of pioglitazone (PIO) and its possible mechanisms on the cultured rabbit aortic smooth muscle cells (VSMCs). METHODS: Proliferation of VSMCs was induced by high concentration of glucose and insulin to mimic insulin resistance (IR) status. Cells proliferation and viability were measured by MTT assay. NO content in culture solution and NOS activities of VSMCs were determined by assay kits, respectively. RESULTS: Proliferation of VSMCs were markedly decreased by PIO in all groups (P <0.01). NO content in culture medium and the activities of total NOS and iNOS of VSMCs were significantly increased by PIO in both normal and mimic IR culture condition (P <0.01). The effects of PIO on VSMCs were stronger in mimic IR culture condition than that in normal culture condition, and these effects of PIO were partially blocked by the NOS inhibitor L-NAME. CONCLUSION: The antiproliferative effects of PIO were partially involved in NO pathway on cultured VSMCs both in normal and mimic IR culture condition.

Key words: nitric oxide, nitric oxide synthase, pioglitazone, vascular smooth muscle cell, insulin resistance

中图分类号:

R977.15

陈鹭颖, 徐江平, 庞建新, 史道华. 一氧化氮部分介导吡格列酮抑制培养兔胸主动脉平滑肌细胞增殖[J]. 中国临床药理学与治疗学, 2005, 10(6): 642-645.

CHEN Lu-ying, XU Jiang-ping, PANG Jian-xin, SHI Dao-hua. Antiproliferative effects of pioglitazone involved of nitric oxide on cultured rabbit aortic smooth muscle cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(6): 642-645.

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