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中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (6): 705-708.

• 研究原著 • 上一篇    下一篇

黄芩苷体外调控乳腺癌细胞表达TIMP2的实验研究

冯正权, 沈敏鹤, 吴良村   

  1. 浙江省中医院肿瘤科, 杭州310006, 浙江
  • 收稿日期:2005-01-27 修回日期:2005-04-29 出版日期:2005-06-26 发布日期:2020-11-12
  • 通讯作者: 冯正权, 男, 医学博士, 主治医师, 从事肿瘤的中西医结合临床工作, 主要研究中医药防治肿瘤转移复发的分子机理及对多药耐药的逆转。Tel:0571-87070931 E-mail: fzhq213@yahoo.com.cn

Research of regulating Bccap-37 cell treated with baicalin on TIMP2 expression in vitro

FENG Zheng-quan, SHEN Min-he, WU Liang-cun   

  1. Zhejiang Hospital of TCM, Hangzhou 310006, Zhejiang, China
  • Received:2005-01-27 Revised:2005-04-29 Online:2005-06-26 Published:2020-11-12

摘要: 目的: 探讨黄芩苷对Bcap-37 细胞生长及TIMP2 表达的影响。方法: 体外细胞培养、MTT 法测定不同浓度黄芩苷对Bcap-37 细胞生长曲线的影响, RT-PCR 、Western blot 方法研究不同浓度黄芩苷对Bcap-37 细胞TIMP2 的影响。结果: 黄芩苷对Bcap-37 细胞的生长抑制呈浓度依赖关系;黄芩苷对Bcap-37 细胞TIMP2 在mRNA 水平、蛋白水平呈浓度依赖关系, 小剂量上调, 高剂量下调。结论: 黄芩苷对人乳腺癌Bcap-37 细胞生长有抑制作用, 可能与肿瘤细胞TIMP2 的下调有关。

关键词: 黄芩, 黄芩苷, 人乳腺癌Bcap-37 细胞株, 基质金属蛋白酶抑制酶2(TIMP2)

Abstract: AIM: To study the effects of baicalin on the growth and TIMP2 expression of bcap-37 cells. METHODS: The effects of baicalin at different concentration on the growth curve of bcap-37 cells were tested by MTT method and TIMP2 expression were analyzed by RTPCR and western blot. RESULTS: Baicalin inhibited the growth of the bcap-37 cells in a concentration relying way. Baicalin up-regulated TIMP2 expression in the bcap37 cells at both Mrna and protein levels at low dose, but down-regulated it at high dose. CONCLUSION: Baicalin can inhibit the growth of human mammary cancer cell line bcap37, and this effect maybe related to downregulate TIMP2 expression in the cancer cells.

Key words: Scutellaria baicalensis Georgi, baicalin, bcap-37 cells, tissue inhibitors of metalloproteinases

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