异普黄酮抗卵巢切除大鼠的骨质疏松与上调腰椎护骨素mRNA 的表达

中国临床药理学与治疗学 ›› 2006, Vol. 11 ›› Issue (10): 1173-1176.

异普黄酮抗卵巢切除大鼠的骨质疏松与上调腰椎护骨素mRNA 的表达

商敏, 杨欣¹, 李亚珍¹, 顾蓓², 吴琼³, 廖秦平¹

北京友谊医院妇产科, 北京 100050;
¹北京大学第一医院妇产科, 北京 100034;
²北京世纪坛医院妇产科, 北京 100038;
³北京首钢总医院妇产科, 北京 100041

收稿日期:2006-06-16 修回日期:2006-07-31 出版日期:2006-10-26 发布日期:2020-11-05
通讯作者: 杨欣, 女, 主任医师, 硕士生导师, 研究方向:妇科内分泌。E-mail:xinyang_2003@sina.com
作者简介:商敏, 女, 博士, 研究方向:妇科内分泌。Tel:13522645309 E-mail:shangmin917@126.com

Ipriflavone up-regulates mRNA expression of osteoprotegerin in lumbar vertebra of ovariectomized rats

SHANG Min, YANG Xin¹, LI Ya-zhen¹, GU Bei², WU Qiong³, LIAO Qin-ping¹

Department of Gynecology andObstetrics, Beijing FriendshipHospital , Beijing 100050 , China;
¹Department of Gynecology and Obstetrics, No.1 Hospital Affiliated to Beijing University, Beijing 100034 , China;
²Department of Gynecology and Obstetrics, Beijing Shijitan Hospital , Beijing 100038 , China;
³Department of Gynecology and Obstetrics, Beijing Shougang General Hospital , Beijing 100041 , China

Received:2006-06-16 Revised:2006-07-31 Online:2006-10-26 Published:2020-11-05

摘要/Abstract

摘要： 目的制造绝经后骨质疏松(postamenopausalosteoporosis, PMOP) 的大鼠动物模型, 研究异普黄酮(Ipriflavone, Ip) 对卵巢切除大鼠腰椎护骨素(OPG) 基因mRNA 表达水平的影响, 探讨其预防和治疗PMOP的作用机制。方法 6 月龄未交配健康雌性Sprague-Dawley 大鼠40 只, 随机分为假手术组(Sham) , 卵巢切除组(OVX) , OVX+戊酸雌二醇组(E2) 和OVX +IP组(Ip) 。灌胃13 周后处死动物, 第四腰椎行骨组织形态计量指标测定, 第二腰椎采用RT-PCR 方法, 对OPG mRNA 表达水平进行检测。结果 OVX组大鼠较假手术组骨小梁体积百分比(TBV%) 显著下降;类骨质体积百分比(OSV%) 明显升高(P<0.05) ;E2 和IP均可使OVX 大鼠的TBV%明显升高, OSV%明显下降;OPG mRNA 表达水平在OVX大鼠骨组织中下调, 与假手术组相比有显著性差异(P<0.05) , E2 和IP均可上调OVX大鼠骨组织OPG mRNA 表达水平(P<0.05) 。结论实验成功制造了大鼠PMOP模型;E2 和IP均通过抑制骨转换发挥预防和治疗PMOP的作用;PMOP的发生与OPG有关, IP和E2 一样, 其预防和治疗骨吸收效应很可能是通过OPG介导的。

关键词: 绝经后骨质疏松, 大鼠, 护骨素, 异普黄酮

Abstract: AIM: To build a postmenopausal osteoporotic model in rats and investigate the effect of ipriflavone on the expression of osteoprotegerin (OPG) mRNA in lumbar vertebra of ovariectomized rats, and to analyze its mechanism in the prevention of postmenopausal osteoporosis.METHODS: Forty female rats at 6 months of age were randomly allocated into 4 groups:sham-operated group, ovariectomized grouP(OVX) , OVX combined valerate estradiol group, and OVX combined ipriflavone group.Rats were sacrificed after they had been lavaged for 13 weeks.The fourth lumbar vertebra was dissected out for histomorphology and the second lumbar vertebra for measurement of mRNA level of OPG by way of reverse transcription polymerase chain reaction technique.RESULTS: The trabecular volume was significantly lower in OVX grouPrats than that in sham grouPrats, while osteoid seam was significantly higher.Valerate estradiol and ipriflavone can increase the trabecular volume and reduced osteoid seam significantly in OVX rats.To compare with sham grouPrats, the mRNA level of OPG decreased significantly in OVX rats, and valerate estrodiol and ipriflavone can up-regulate the expression level of OPG mRNA significantly.CONCLUSION: Our experiment built the postmenopausal osteoporotic model successfully.Valerate estradiol and ipriflavone can prevent postmenopausal osteoporosis by its inhibitory effect on bone turnover.OVX-induced bone loss was associated with OPG.Iprif lavone,just like valerate estradiol, perhaps has its antiresorbative effect on bone by the signal of OPG.Estrogen and ipriflavone can up-regulate the mRNA expression of osteoprotegerin in the lumbar vertebra tissue of ovariectomized rats.

Key words: postmenopausal osteoporosis, rat, osteoprotegerin, ipriflavone

中图分类号:

R965.2
R966

商敏, 杨欣, 李亚珍, 顾蓓, 吴琼, 廖秦平. 异普黄酮抗卵巢切除大鼠的骨质疏松与上调腰椎护骨素mRNA 的表达[J]. 中国临床药理学与治疗学, 2006, 11(10): 1173-1176.

SHANG Min, YANG Xin, LI Ya-zhen, GU Bei, WU Qiong, LIAO Qin-ping. Ipriflavone up-regulates mRNA expression of osteoprotegerin in lumbar vertebra of ovariectomized rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(10): 1173-1176.

参考文献

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