匹维溴胺对束缚应激大鼠结肠不同部位和肌层平滑肌条的作用

中国临床药理学与治疗学 ›› 2006, Vol. 11 ›› Issue (3): 314-318.

匹维溴胺对束缚应激大鼠结肠不同部位和肌层平滑肌条的作用

李翠萍, 刘诗, 徐三平, 钱伟, 侯晓华

华中科技大学同济医学院附属协和医院消化内科,武汉 430022,湖北

收稿日期:2005-12-01 修回日期:2006-01-21 出版日期:2006-03-26 发布日期:2020-12-04

Effect of Pinaverium Bromide on different layers and segments of colonic smooth muscle strips in wrap restraint stress rats

LI Cui-ping, LIU Shi, XU San-ping, QIAN Wei, HOU Xiao-hua

Department of Gastroenterology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,Hubei,China

Received:2005-12-01 Revised:2006-01-21 Online:2006-03-26 Published:2020-12-04
Contact: LIU Shi,female,MD,associate professor,tutor of master,engaged in gast rointestinal motility.Tel:027-85726381 　E-mail:shiliugao@yahoo.com
About author:LI Cui-ping,female,MD,engaged in gastroint estinal motility.Tel:027-62420503 　E-mail:cuipingli76@yahoo.com.cn

摘要/Abstract

摘要： 目的评价匹维溴胺对束缚应激大鼠离体结肠平滑肌条的影响并探讨其对各型IBS可能的作用机理。方法成年SD大鼠随机分为模型组和对照组,模型组用自制硬塑胶模具束缚2h。分别制作两组大鼠近端及远端结肠的环行肌和纵行肌条共64条(每组肌条32个,各肌层8个)。采用离体平滑肌条器官浴槽实验方法,观察不同浓度匹维溴胺对平滑肌条自发性收缩活动的影响。结果匹维溴胺对模型组和对照组离体结肠平滑肌条自发性收缩活动均表现为抑制作用,但对模型组效应较对照组显著(近端结肠28.54±4.82vs7.48±1.65,21.75±1.00vs12.56±3.15;远端结肠15.71±5.27vs3.89±1.16,20.16±3.16vs7.56±1.96)(P<0.05)。匹维溴胺对近端结肠平滑肌收缩的抑制作用较远端结肠显著(P<0.05)。匹维溴胺对相同部位结肠环行肌与纵行肌的抑制作用无显著性差异(P>0.05)。结论匹维溴胺能抑制束缚应激大鼠离体结肠平滑肌的收缩,且对近端结肠收缩的抑制作用显著高于远端结肠;但在相同部位,对环行肌和纵行肌收缩的抑制作用无显著性差异。

关键词: 匹维溴胺, 束缚应激, 大鼠, 结肠平滑肌条, 自发性收缩

Abstract: AIM: To evaluate effects of Pinaverium Bromide on different segments and layers of colonic smooth muscle in wraPrestraint stress (WRS) rats and explore its possible therapeutic mechanism on different types of irritable bowel syndrome (IBS).METHODS: Adult SD rats were randomly divided into model group(wraPrestraint stress group) and control group.Colonic smooth muscle strips were made from different segments and layers in two groups.The spontaneous contraction activities of colonic longitudinal/circular muscle (LM/CM)strips of rats were observed with organ bath system before and after addition of series concentrations of pinaverium.RESULTS: Pinaverium Bromide caused concentrationdependent inhibition of colonic smooth muscle,the inhibitory effect of pinaverium in model grouPwas significantly stronger than that in control group(proximal colon:28.54±4.82 vs 7.48±1.65,21.75±1.00 vs 12.56±3.15;distal colon:15.71±5.27 vs 3.89±1.16,20.16±3.16 vs 7.56±1.96) (P<0.05).Compared with that of distal colon,inhibitory effect of pinaverium was significantly higher of proximal colon (P<0.05).For the inhibition of pinaverium,there was no significant difference between LM and CM strips in the same intestinal segments (P>0.05).CONCLUSION: Effects of Pinaverium Bromide on different colonic muscle layers and segments in WRS rats is probably related with its therapeutic mechanism on different types of IBS.

Key words: Pinaverium Bromide, wrap restraint stress, smooth muscle strip, colonic motility

中图分类号:

R965.2

李翠萍, 刘诗, 徐三平, 钱伟, 侯晓华. 匹维溴胺对束缚应激大鼠结肠不同部位和肌层平滑肌条的作用[J]. 中国临床药理学与治疗学, 2006, 11(3): 314-318.

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