建立酒精性肝纤维化高血脂双重疾病大鼠模型的快速方法

中国临床药理学与治疗学 ›› 2006, Vol. 11 ›› Issue (6): 650-654.

建立酒精性肝纤维化高血脂双重疾病大鼠模型的快速方法

刘桂良, 高利忠, 蓝岚秀子, 陈丁丁

中国药科大学药理研究室, 南京 210009, 江苏

收稿日期:2006-03-01 修回日期:2006-03-31 出版日期:2006-06-26 发布日期:2020-12-04
通讯作者: 陈丁丁,男,副教授,研究方向:心血管药理。Tel Fax:025-83271264 E-mail:besson@besson.com.cn
作者简介:刘桂良,女,研究生,研究方向:心血管药理学。

Quick method for simultaneously establishing hepatic fibrosis and hyperlipemia double diseases model in rats

LIU Gui-liang, GAO Li-zhong, LANLAN Xiu-zi, CHEN Ding-ding

Research Division of Pharmacology, China Pharmaceutical University, Nanjing 210009, Jiansu, China

Received:2006-03-01 Revised:2006-03-31 Online:2006-06-26 Published:2020-12-04

摘要/Abstract

摘要： 目的: 研究一种快速建立大鼠酒精性肝纤维化同时具有高血脂模型的新方法。方法: SD 大鼠ig60%乙醇(10 ml·kg^-1)、胆固醇(1 g·kg^-1)、猪油(5 g·kg^-1) 混合物15 ml·kg^-1·d^-1, 共31 d;Kivirikko法测定胶原含量;F3B 天狼猩红荧光偏振光法测定组织胶原;实时荧光PCR 法定量Ⅰ、Ⅳ型胶原基因表达;组织病理学分析;自动生化分析仪测定血液生化指标。结果: 病理模型组大鼠肝脏(Ⅰ 型) 胶原含量、Ⅳ型胶原基因表达水平、肝脏脂肪含量与血脂水平较正常对照组显著升高(P<0.01)。组织病理学分析可见病理组肝脏病变明显。结论: 建立的酒精性肝纤维化-高血脂双重疾病大鼠模型, 快速稳定且简便易行, 适于抗脂肪肝、抗肝纤维化与降血脂药物干预研究。

关键词: 肝纤维化, 脂肪肝, 高血脂, 酒精, 胶原

Abstract: AIM: To establish a relatively simple but reliable rat model of liver fibrosis.Methods: A mixture composed of ethanol (60%)、lard (5 g·kg^-1) and cholesterol(1 g·kg^-1) was supplied in a concentration of 15 ml·kg^-1·d^-1 for one month.Animals are concurrently supplied a normal diet.Results: Biochemical analysis and polarization microscopy combined with image analysis liver sections stained with F3B Sirius red revealed that the whole collagen quantity and type Ⅰ collagen in mixturetreated rat livers were increased very significantly than those of control group.Tissue pathological assay revealed middle to heavy degree cellular swelling and obvious sinus hepaticus hyperaemia in liver.Real time PCR assay showed that type Ⅳ collagen gene expression in model rat liver was very significantly elevated than that of normal rat.The triglyceride contents of liver and blood in model rats were significantly elevated than those of normal control rats.Conclusion: The model provides a convenient method for the production of liver fibrosis and hyperlipemia, and it may be useful for the study of the medicines treating hepatic fibrosis adiposis-hepatca and hyperlipemia.

Key words: hepatic fibrosis, adiposis-hepatica, hyperlipemia, alcohol, collagen

中图分类号:

R965.1

刘桂良, 高利忠, 蓝岚秀子, 陈丁丁. 建立酒精性肝纤维化高血脂双重疾病大鼠模型的快速方法[J]. 中国临床药理学与治疗学, 2006, 11(6): 650-654.

LIU Gui-liang, GAO Li-zhong, LANLAN Xiu-zi, CHEN Ding-ding. Quick method for simultaneously establishing hepatic fibrosis and hyperlipemia double diseases model in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(6): 650-654.

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