胡黄连甙Ⅱ和神经生长因子对H2O2 损伤的PC12 细胞的协同保护作用

中国临床药理学与治疗学 ›› 2007, Vol. 12 ›› Issue (1): 32-37.

胡黄连甙Ⅱ和神经生长因子对H₂O₂ 损伤的PC12 细胞的协同保护作用

张小东, 刘建文, 李婷, 曹艳, 郭明川

华东理工大学药学院, 上海 200237

收稿日期:2006-05-08 修回日期:2006-11-13 出版日期:2007-01-26 发布日期:2020-10-26

Synergistic protective effect of picrosideⅡand NGF on PC12 cells against oxidative stress induced by H₂O₂

ZHANG Xiao-dong, LIU Jian-wen, LI Ting, CAO Yan, GUO Ming-chuan

East China University of Science and Technology, School of Pharmacy, Shanghai 200237, China

Received:2006-05-08 Revised:2006-11-13 Online:2007-01-26 Published:2020-10-26
Contact: LIU Jian-wen, male, PhD &M.D, professor, mentor of doctor, specialized in the study of effects and mechanisms of natural products. Tel:021-64252044 E-mail: liujian@ecust.edu.cn
About author:ZHANG Xiao-dong, male, master, specialized in biochemistry andmolecular biology.Tel:021-64252262 　E-mail:zhangxd128@163.com
Supported by:
Shanghai SK-foundation project(2003008t)

摘要/Abstract

摘要： 目的: 考察胡黄连甙Ⅱ 和NGF 对双氧水损伤细胞的协同保护作用。方法: 以CDCFH 方法评价活性氧水平, MTT 方法、细胞形态观察以及LDH 漏出实验评价药物对细胞损伤的修复作用。结果: 25μg/mL胡黄连甙Ⅱ 和2 ng/mL NGF 显著改善H₂O₂ 诱导的PC12 细胞损伤, 同时有效降低细胞内ROS 。细胞形态改善、细胞活力提高、ROS 清除作用均表明胡黄连甙Ⅱ 和NGF 协同保护氧化应激对神经细胞的损伤。结论: 胡黄连甙Ⅱ 和NGF 的协同作用能有效治疗神经性疾病。

关键词: 双氧水, 神经生长因子, 胡黄连甙Ⅱ, 活性氧

Abstract: AIM: To study the synergistic protective effect of picroside Ⅱ and NGF for the oxidative stress on PC12 cells induced by hydrogen peroxide (H₂O₂). METHODS: The fluorescent probe 6-carboxy-2', 7' -dichlorodihydrofluorescein (CDCFH) was used to assess the intracellular reactive oxygen species (ROS) level, and MTT assay, morphological observation as well as lactate dehydrogenase (LDH) leakage were conducted to measure cellular injury. RESULTS: The H₂O₂-induced cytotoxicity was significantly attenuated in the presence of picroside Ⅱ (25 μg/mL) and NGF (2 ng/mL).Cultures with this combined treatment possessed decreased level of ROS while increased cell survival, as compared to that of picroside Ⅱ or NGF alone-treated cells.Accordingly, it was concluded that their synergistic protective activities against oxidative stress in vitro were demonstrated in various aspects including reversing morphological changes, enhancing the ability of cell proliferation and ROS scavenging. CONCLUSION: Such action supports the therapeutic potential of picroside Ⅱ and NGF in treating nervous disorders based on their synergistic effect.

Key words: hydrogen peroxide, nerve growth factor, picrosideⅡ, reactive oxygen species

中图分类号:

R965.2

张小东, 刘建文, 李婷, 曹艳, 郭明川. 胡黄连甙Ⅱ和神经生长因子对H₂O₂ 损伤的PC12 细胞的协同保护作用[J]. 中国临床药理学与治疗学, 2007, 12(1): 32-37.

ZHANG Xiao-dong, LIU Jian-wen, LI Ting, CAO Yan, GUO Ming-chuan. Synergistic protective effect of picrosideⅡand NGF on PC12 cells against oxidative stress induced by H₂O₂[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2007, 12(1): 32-37.

参考文献

[1] Zhao HM, Liu XF, Mao XW, et al. Intranasal delivery of nerve growth factor to protect the central nervous system against acute cerebral infarction[J]. ChinMed Sci J, 2004;19: 257-261.
[2] Poduslo JF, Curran GL, Gill JS. Putrescine-modified nerve growth factor: bioactivity, plasma pharmacokinetics, bloodbrain nerve barrier permeability, and nervous system biodistribution[J]. J Neurochem, 1998;71: 1651-1660.
[3] Li P, Matsunaga K, Ohizumi Y. Nerve growth factor-potentiating compounds from Picrorhizae Rhizoma[J]. Biol Pharm Bull, 2000;23: 890-892.
[4] Li P, Matsunaga K, Yamakuni T, et al. PicrosidesⅠand Ⅱ, selective enhancers of the mitogen-activated protein kinase-dependent signaling pathway in the action of neuritogenic substances on PC12D cells[J]. Life Sci, 2002;71: 1821-1835.
[5] Middlemiss PJ, Glasky AJ, Rathbone MP, et al. AIT-082, a unique purine derivative, enhances nerve growth factor mediated neurite outgrowth from PC12 cells[J]. Neurosc Lett, 1995;199: 131-134.
[6] Pradines A, Magazin M, Schiltz P, et al. Evidence for nerve growth factor-potentiating activities of the nonpeptidic compound SR 57746A in PC12 cells[J]. J Neurochem, 1995;64: 1954-1964.
[7] Angus WG, Contreras ML. Aroclor 1254 alters the binding of 125 I-labeled nerve growth factor in PC12 cells[J]. Neurosci Lett, 1995;191: 23-26.
[8] Ito M, Sakai N, Ito K, et al. A novel fungal metabolite NG-061 enhances and mimics neurotrophic effect of nerve growth factor (NGF) on neurite outgrowth in PC12 cells[J]. J Antibiot, 1999;52: 224-230.
[9] Li P, Matsunaga K, Yamamoto K, et al. Nardosinone, a novel enhancer of nerve growth factor in neurite outgrowth from PC12D cells[J]. Neurosci Lett, 1999;273: 53-56.
[10] Li P, Matsunaga K, Yamakuni T, et al. Potentiation of nerve growth factor-action by picrosides I and Ⅱ, natural iridoids, in PC12D cells[J]. Eur J Pharmacol, 2000;406: 203-208.
[11] Scandalios JG. The rise of ROS[J]. Trends Biochem Sci, 2002;27: 483-486.
[12] Machlin LJ, Bendich A. Free radical tissue damage: protective role of antioxidant nutrient[J]. FASEB J, 1987;1: 441-445.
[13] Huang Y, Dong Z. Oxidative stress and Alzheimer' s disease[J]. Chin J Clin Pharmacol Ther, 2005;10: 485-488.
[14] Hu J, Hu G. Progress in the strudy of glutathione in Parkinson' s disease[J]. Chin J Clin Pharmacol Ther, 2003;8: 481-484.
[15] Bilici M, Efe H, KorogluMA, et al. Antioxidative enzyme activities and lipid peroxidation in major depression: alteration by antidepressant treatments[J]. J Affect Disorders, 2001;64: 43-51.
[16] Chandra J, Samali A, Orrenius S. Triggering and modulation of apoptosis by oxidative stress[J]. Free Radical Bio Med, 2000;29: 323-333.
[17] Manton KG, Volovik S, Kulminski A. ROS effects on neurodegeneration in Alzheimer' s disease and related disorders: on environmental stresses of ionizing radiation[J]. Curr Alz Res, 2004;1: 277-293.
[18] Woodruff RH, Franklin RJ. Growth factors and remyelination in the CNS[J]. Histol Histopathol, 1997;12: 459-466.
[19] Patrick CWJ, Kukreti S, Mcintire LV. Quantitative effects of peripheral monocytes and nerve growth factor on CNS neural morphometric outgrowth parameters in vitro[J]. Exp Neurol, 1996;138: 277-285.
[20] Johnson EM, Chang JY, Koike T, et al. Why do neurons die when deprived of tropic factors[J] ? Neurobiol Aging, 1989;10: 549-552.
[21] Brinton RD, Yamazaki RS. Advances and challenges in the prevention and treatment of Alzheimer' s disease[J]. Pharm Res, 1998;15: 386-398.
[22] Senthil Kumar SH, Anandan R, Devaki T, et al. Cardioprotective effects of Picrorrhiza kurroa against isoproterenol-induced myocardial stress in rats[J]. Fitoterapia, 2001;72: 402-405.
[23] Singh AK, Mani H, Seth P, et al. Picroliv preconditioning protects the rat liver against ischemia-reperfusion injury[J]. Eur J Pharmacol, 2000;395: 229-239.
[24] Sampath D, Jackson GR, Werrbach-perez K, et al. Effects of nerve growth factor on glutathione peroxidase and catalase in PC12 cells[J]. J Neurochem, 1994;62: 2476-2479.

胡黄连甙Ⅱ和神经生长因子对H₂O₂ 损伤的PC12 细胞的协同保护作用

Synergistic protective effect of picrosideⅡand NGF on PC12 cells against oxidative stress induced by H₂O₂

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	王亚丽, 霍续磊, 陈峰, 李生兰, 郑晓红, 王灿, 李文斌. 硝呋莫司抗肿瘤作用的研究进展[J]. 中国临床药理学与治疗学, 2022, 27(8): 955-960.
[2]	李雯霞, 方柳圆, 钱申贤. 铁死亡的机制及其在淋巴瘤中的研究进展[J]. 中国临床药理学与治疗学, 2022, 27(3): 314-321.
[3]	李莹, 姚伟, 王萌, 余志宏, 龚园其, 蓝海兵, 齐协飞. 鸢尾素通过NF-κB和JNK信号通路减轻屋尘螨诱导的气道上皮细胞炎症及凋亡的实验研究[J]. 中国临床药理学与治疗学, 2022, 27(10): 1106-1112.
[4]	严洁萍，章水均，张国兵，胡颖，罗丹，江红娟，李敏静. 丁苯酞对S-亚硝基化谷胱甘肽诱导脑微血管内皮细胞损伤的保护作用及机制研究[J]. 中国临床药理学与治疗学, 2019, 24(5): 517-522.
[5]	陈峰，邹懿，胡波，胡松，陈勇，高大新. 鼠神经生长因子对坐骨神经痛大鼠背根神经节磷酸化p38MAPK表达的影响[J]. 中国临床药理学与治疗学, 2019, 24(4): 411-417.
[6]	蒋豪明，陈翔，李珍，顾承萍. 单唾液酸四己糖神经节苷脂联合鼠神经生长因子对新生儿缺氧缺血性脑病的临床研究[J]. 中国临床药理学与治疗学, 2019, 24(3): 301-306.
[7]	刘若海，卢园园，李丽伶，沈露露，曹红，李军，李旭. D-4F在七氟烷处理对急性高糖血症小鼠血管保护中的作用及机制[J]. 中国临床药理学与治疗学, 2017, 22(7): 743-748.
[8]	商宏恺，傅爱萍，陈露芳，仝进毅，斯奇. 地塞米松对人胎盘葡萄糖转运功能的作用及机制[J]. 中国临床药理学与治疗学, 2017, 22(11): 1221-1226.
[9]	江爱娟，申国明，王浩，李道卫，王芹，何莹. 益气活血通络方对糖尿病周围神经病变大鼠背根神经节p75NTR和TrkA表达的影响[J]. 中国临床药理学与治疗学, 2016, 21(2): 121-124.
[10]	苏丽，王锃，黄菲，蓝瑞隆，石丹，陈纯，张鲁榕，张纬建，洪金省. 甘草次酸减轻放射性炎症反应的作用机制研究[J]. 中国临床药理学与治疗学, 2016, 21(10): 1088-1094.
[11]	张雪, 谢海棠, 吴兰香, 沈杰, 周宏灏. 异硫氰酸苯乙酯通过上调ROS激活p53诱导MCF-7细胞凋亡机制的研究[J]. 中国临床药理学与治疗学, 2015, 20(7): 754-758.
[12]	唐若愚, 屈莺歌, 许洪涛, 阎澜, 颜天华, 姜远英, 曹永兵. 红景天苷防治急性高原病的作用及机制[J]. 中国临床药理学与治疗学, 2015, 20(7): 822-827.
[13]	唐杰, 胡伟, 吴繁荣, 葛金芳, 潘春晓, 高文凡, 陈飞虎. 酸性条件下神经生长因子对大鼠关节软骨细胞酸敏感离子通道1a表达的影响[J]. 中国临床药理学与治疗学, 2015, 20(10): 1081-1086.
[14]	杨国栋, 张晓英, 冯志松, 贺国斌, 江毅敏. N-异喹啉-3-羧酸-L-亮氨酸苄酯抗人食道癌作用的研究[J]. 中国临床药理学与治疗学, 2014, 19(8): 877-881.
[15]	甄东户, 刘丽娟, 成建国, 汤旭磊. 二甲双胍对糖基化终末产物诱导的成骨细胞氧化应激和凋亡的影响[J]. 中国临床药理学与治疗学, 2013, 18(7): 743-748.