重组人角质细胞生长因子-2对大鼠皮肤烫伤的治疗作用及其可能机制探讨

中国临床药理学与治疗学 ›› 2007, Vol. 12 ›› Issue (6): 639-644.

重组人角质细胞生长因子-2对大鼠皮肤烫伤的治疗作用及其可能机制探讨

袁海虹¹, 周薇¹, 荣征星¹, 廖镇江², 张勤², 乔亮², 陈红专¹

¹上海交通大学医学院基础医学院药理学教研室,²上海交通大学医学院瑞金临床医学院烧伤研究所, 上海200025

收稿日期:2007-04-12 修回日期:2007-05-16 发布日期:2020-11-09
通讯作者: 陈红专, 男, 博士, 教授, 博士生导师, 研究方向:神经药理学、肿瘤分子药理学和新药R&D。Tel:021-63846590-776451 E-mail:yaoli@shsmu. edu. cn
作者简介:袁海虹, 女, 硕士研究生, 研究方向:新药R&D。Tel:021-63846590-776450 E-mail:yuanhaihong @126.com
基金资助:
上海市科委生物医药重点科研攻关项目(064319027)

Effect of recombinant human keratinocyte growth factor-2 on burn wound healing in rats and its probable mechanisms of action

YUAN Hai-hong¹, ZHOU Wei¹, RONG Zheng-xing¹, LIAO Zhen-jiang², ZHANG Qin², QIAO Liang², CHEN Hong-zhuan¹

¹Department of Pharmacology, School of Basic Medical Science, Shanghai Jiao Tong University, 2Department of Burns,Ruijing Hospital, Medical College of Shanghai Jiao Tong University, Shanghai 200025, China

Received:2007-04-12 Revised:2007-05-16 Published:2020-11-09

摘要/Abstract

摘要： 目的:研究重组人角质细胞生长因子-2(rh-KGF-2) 对大鼠皮肤烫伤愈合的影响及其可能的作用机制。方法:建立大鼠深Ⅱ度烫伤模型, 每天局部给药1 次, 连续15 d, 每4 d 测量伤口面积并计算愈合率, d 16 观察新生上皮面积、厚度和上皮细胞迁移距离。体外培养HaCaT 细胞, MTT 法检测不同浓度的rhKGF-2 对HaCaT 细胞增殖率的影响;划痕实验检测rhKGF-2 对HaCaT 细胞移行的影响。结果:rh-KGF-2 对大鼠皮肤深Ⅱ度烫伤的愈合有明显的促进作用, 使新生上皮面积、平均厚度和上皮细胞移行距离等明显增加。rhKGF-2 可刺激HaCaT 细胞增殖,集落形成率增加(P<0. 05), 且细胞呈明显的增殖形态, 形成的集落较大, 集落内细胞多呈星状, 胞内染色质丰富;rhKGF-2 能显著促进创伤周围细胞向创面中央移行(P<0. 01) 。结论:rhKGF-2 能明显加快大鼠烫伤创面愈合, 可能作用机理为通过促进表皮细胞增殖和移行, 加速创面皮肤再上皮化。

关键词: 角质细胞生长因子-2, 烫伤, HaCaT 细胞, 增殖, 移行

Abstract: AIM:To investigate the effect of recombinant human keratinocyte growth factor-2 (rhKGF-2) on skin wound healing in rats and its possible mechanism.METHODS:Deep Ⅱ degree burn wound model was established in rats. The wound areas, the thickness, migration distances and areas of neoepithelia tissue were detected with IMAGE-PRO express morphological analysis software after Hematoxylin and Eosin staining. MTT and clone forming assay were used to measure the HaCaT cells proliferation. The keratinocyte migration and cell wound closure were observed in the scratch experiment.RESULTS: rhKGF-2 obviously accelerated the healing rate of deep II degree burn wound with improved reepidermidation. Increases in migration distance, average area and thickness of neoepithelium were found in a dose-dependent manner. In addition, rhKGF-2 increased HaCaT cells proliferation rate, migration and clone forming efficiency in a concentration dependent manner (P<0. 05).CONCLUSION: These results show that rhKGF-2 significantly accelerates wound healing partly through the enhancement of keratinocyte proliferation and migration.

Key words: keratinocyte growth factor-2, wound healing, HaCaT, proliferation, migration

中图分类号:

R965. 2
R644

袁海虹, 周薇, 荣征星, 廖镇江, 张勤, 乔亮, 陈红专. 重组人角质细胞生长因子-2对大鼠皮肤烫伤的治疗作用及其可能机制探讨[J]. 中国临床药理学与治疗学, 2007, 12(6): 639-644.

YUAN Hai-hong, ZHOU Wei, RONG Zheng-xing, LIAO Zhen-jiang, ZHANG Qin, QIAO Liang, CHEN Hong-zhuan. Effect of recombinant human keratinocyte growth factor-2 on burn wound healing in rats and its probable mechanisms of action[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2007, 12(6): 639-644.

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