N-乙酰半胱氨酸对大鼠肝星状细胞中的瘦素与Ⅰ型胶原水平的影响

中国临床药理学与治疗学 ›› 2007, Vol. 12 ›› Issue (8): 920-922.

N-乙酰半胱氨酸对大鼠肝星状细胞中的瘦素与Ⅰ型胶原水平的影响

陈益平, 汪洪娇, 徐志伟, 陈均亚, 石海矾, 狄军波, 李素华

温州医学院附属育英儿童医院感染科, 温州 325027, 浙江

收稿日期:2007-07-16 修回日期:2007-08-20 出版日期:2007-08-26 发布日期:2020-10-27
作者简介:陈益平,医学硕士,副主任医生,研究方向:儿童肝炎与肝病。Tel:13857716909 E-mail:cccyp@163.com
基金资助:
温州市科技局课题(Y2004A071)

Effects of N-acetylcysteine on expression of leptin and type Ⅰ collangen in cultured rat hepatic stellate cells

CHEN Yi-ping, WANG Hong-jiao, XIU Zhi-wei, CHEN Jun-ya, SHI Hai-fan, DI Jun-bo, LI Su-hua

Department of Pediatrics Infectious Disease, the Yuying Affiliated Children's Hospital of Wenzhou Medical College, Wenzhou 325027, Zhejiang, China

Received:2007-07-16 Revised:2007-08-20 Online:2007-08-26 Published:2020-10-27

摘要/Abstract

摘要： 目的: 研究N-乙酰半胱氨酸(N-acetylcysteine,NAC) 影响大鼠肝星状细胞(hepatic stellate cell,HSC) 增殖, 及其对瘦素与Ⅰ 型胶原蛋白分泌的变化。方法: HSC 以1×10⁵ mL 浓度接种于96 孔培养板, 每孔100 μL, 进行以下实验分组:NAC Ⅰ、Ⅱ、Ⅲ组, 加入NAC 使得各组培养液中NAC 浓度分别是10、20、40 mmol/L;加生理盐水为对照组。培养48 h终止后吸取上清液-20 ℃ 冰冻保存, ELISA 法检测细胞培养上清液瘦素与Ⅰ 型胶原蛋白水平;MTT 法观察细胞增殖情况。结果: NAC Ⅲ组平均吸光度低于NAC Ⅰ 组和对照组(P<0.05);NAC Ⅱ、Ⅲ组其HSC 培养上清液瘦素水平均显著低于对照组(P<0.01);NAC 各组Ⅰ 型胶原蛋白水平均显著低于对照组(P<0.01) 。结论: HSC 在NAC 作用下增殖减少;瘦素与Ⅰ 型胶原蛋白分泌受到抑制, 这可能是NAC抗纤维化的机理之一。

关键词: 肝星状细胞, N-乙酰半胱氨酸, 瘦素, 胶原蛋白

Abstract: AIM: To study the effects of N-acetylcysteine on the proliferation of rat hepatic stellate cells (HSCs) and the expression variation of leptin and type Ⅰ collagen. METHODS: HSCs were seeded at a density of 1×10⁵ cells mL per well in 96-well plates, which were grouped as follows:NAC Ⅰ, Ⅱ, Ⅲ groups(the concentration of NAC in incubation was 10, 20, 40 mmol/L respectively);Normal saline (NS) was added in the control group.The incubation liquid was extracted after cultured 48 h and was frozen at-20 ℃.Leptin and the synthesis of type Ⅰ collagen were measured by enzyme linked immunosorbent assay(ELISA) and the HSC proliferation was measured by MTT assay. RESULTS: The average absorbance of NAC Ⅲ was signficantly lower than that of the control group(P<0.05).The average type Ⅰ collagen in each NAC group was significantly lower that of the control's(P<0.01). CONCLUSION: HSC proliferation is decreased and the secretion of leptin and type Ⅰ collagen is inhibited after NAC pretreatment.

Key words: hepatic stellate cell, N-acetylcysteine, leptin, type Ⅰ collagen

中图分类号:

R965.2

陈益平, 汪洪娇, 徐志伟, 陈均亚, 石海矾, 狄军波, 李素华. N-乙酰半胱氨酸对大鼠肝星状细胞中的瘦素与Ⅰ型胶原水平的影响[J]. 中国临床药理学与治疗学, 2007, 12(8): 920-922.

CHEN Yi-ping, WANG Hong-jiao, XIU Zhi-wei, CHEN Jun-ya, SHI Hai-fan, DI Jun-bo, LI Su-hua. Effects of N-acetylcysteine on expression of leptin and type Ⅰ collangen in cultured rat hepatic stellate cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2007, 12(8): 920-922.

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