乙酰半胱氨酸对大鼠酒精性肝损伤的保护作用

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (5): 517-520.

乙酰半胱氨酸对大鼠酒精性肝损伤的保护作用

王福根^1,2, 俞佳³, 席建军¹

¹杭州市第六人民医院药剂科, 杭州310014, 浙江;
²浙江大学药学院, 杭州310058, 浙江;
³浙江省人民医院药剂科, 杭州310014, 浙江

收稿日期:2008-03-05 修回日期:2008-04-02 发布日期:2020-11-09
作者简介:王福根, 男, 硕士在读, 主管药师, 研究方向:肝炎药物的药理研究。Tel:0571-85463955 E-mail:WFG85463955 @126. com
基金资助:
杭州市卫生局重点资助项目(2006A12)。

Protection of N-acetylcysteine on alcoholic liver injury

WANG Fu-gen^1,2, YU Jia³, XI Jian-jun¹

¹Sixth People's Hospital of Hangzhou, Hangzhou 310014, Zhejiang, China;
²College of Pharmaceutical Science, Zhejiang University, Hangzhou 310058, Zhejiang, China;
³People's Hospital of Zhejiang, Hangzhou 310014, Zhejiang, China

Received:2008-03-05 Revised:2008-04-02 Published:2020-11-09

摘要/Abstract

摘要： 目的:探讨乙酰半胱氨酸对大鼠酒精性肝损伤的保护作用。方法:SD 大鼠70 只, 随机分成6 组:正常组(n =10, 等渗盐水灌胃和腹腔注射)、模型组(n =12, 酒精8. 8 g·kg^-1·d^-1 灌胃)、治疗组(n =12, 酒精8. 8 g·kg^-1·d^-1灌胃, 造模同时腹腔注射0.156、 0. 312、 0. 625 g/kg 乙酰半胱氨酸)、阳性对照组(n =12, 酒精8. 8 g·kg^-1·d^-1 灌胃, 造模同时腹腔注射硫普罗宁16. 5 mg/kg^-1·d^-1)。10 周末处死大鼠, 检测血清ALT、 AST 活性和肝组织谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)、丙二醛(MDA)含量, 观察肝组织病理变化。结果:模型组大鼠血清ALT、 AST 活性、肝组织MDA 水平较正常组升高, 肝组织GSH-PX、 SOD较正常组降低, 差异有统计学意义(P <0. 05);治疗组、阳性对照组与模型组相比,ALT、 AST、 MDA有明显的降低, 肝组织GSH-PX、 SOD 水平有明显的升高, 差异有统计学意义(P <0. 05)。HE 染色显示模型组大鼠肝细胞浆出现不同程度脂肪变性, 肝小叶可见肝细胞点状坏死, 治疗组和阳性对照组脂肪变性和炎症程度轻于模型组。结论:乙酰半胱氨酸对大鼠酒精性肝损伤具有一定的保护作用。

关键词: 肝疾病, 酒精性, 乙酰半胱氨酸, 大鼠

Abstract: AIM:To reproduce an experimental model of alcoholic in rats and to investigate the protective effects of N-acetylcysteine on alcoholic liver disease. METHODS:70 male Sprague-Dawley rats were randomly divided into 6 groups: control group (gastric infusion and intraperitoneal injection with normal saline), alcohol group (gastric infused with 55% of ethanol once a day with a dose of 8. 8 g/kg for 10 weeks), NAC groups(gastric infusion with alcohol same as in the alcohol group and intraperitoneal injection with NAC with different doses), positive control group (gastric infusion with alcohol same as in the alcohol goup and intraperitoneal injection with tiopronin of 16. 5 mg/kg).At the end of 6 weeks blood samples were collected through caudal vein. At the end of 10 weeks the rats were sacrificed then blood samples were collected. Liver samples were obtained for detection and for routine histological examination. RESULTS:(1) The levels of the two transaminases(ALT&AST), and Malondialdehyde were elevated in alcohol group, and the Glutathione peroxidase and superoxide dismutase levels were lower than control group. The difference is significant. NAC significantly mitigated the increase of ALT、 MDA、 AST, and significantly elevated GSH-PX, SOD induced by the alcohol. (2)Histological changes of the liver injury that piecemeal or focal necrosis of hepatocytes was present in the centrilobular area. In the NAC and positive control group, the severity of the histological changes was significantly milder. CONCLUSION: The results of this study revealed that N-acetylcysteine protected from alcoholic liver disease.

Key words: liver disease, alcoholic, N-acetylcysteine, rat

中图分类号:

R965. 2

王福根, 俞佳, 席建军. 乙酰半胱氨酸对大鼠酒精性肝损伤的保护作用[J]. 中国临床药理学与治疗学, 2008, 13(5): 517-520.

WANG Fu-gen, YU Jia, XI Jian-jun. Protection of N-acetylcysteine on alcoholic liver injury[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(5): 517-520.

[1]	许秀荷, 何晓丽, 周佳顺, 潘丽珠, 周卓君, 李佳悦, 王彩霞, 袁伟, 朱桂琦. 蜂蜜对大鼠血尿酸水平及肾损伤研究[J]. 中国临床药理学与治疗学, 2023, 28(7): 743-750.
[2]	汪东辉, 蒋素文, 胡爱荣, 朱波, 何哲耘, 张露侃, 王家岚, 范莹, 林恳. 桑葚改善大鼠非酒精性脂肪性肝病的氧化应激损伤[J]. 中国临床药理学与治疗学, 2023, 28(6): 609-616.
[3]	李红琴, 曹利民, 金满意, 王建龙, 柴晓萍, 田苗苗. 牵正散不同剂型改善大鼠面神经损伤作用研究[J]. 中国临床药理学与治疗学, 2023, 28(6): 617-623.
[4]	衡冰冰, 杨丹峰, 戴舒阳, 上官王宁. 丙泊酚对皮下注射氯喹所致瘙痒大鼠脊髓TRPV1和CB1受体的影响[J]. 中国临床药理学与治疗学, 2023, 28(10): 1101-1108.
[5]	张静, 高丹阳, 于康, 林晓婉, 曹颖, 刘晓, 缪慧慧, 李天佐. 不同吸入麻醉药对新生期大鼠神经胶质细胞活化的影响[J]. 中国临床药理学与治疗学, 2022, 27(12): 1391-1399.
[6]	张远金, 黄盛博, 刘洁, 王昕. 药物代谢创新模型：基因编辑大鼠和小肠3D类器官[J]. 中国临床药理学与治疗学, 2021, 26(8): 914-922.
[7]	王香溢, 盛洁, 王秀, 郭岩, 陈志武. UPLC-MS/MS法测定氘代氯化琥珀胆碱在大鼠体内分布[J]. 中国临床药理学与治疗学, 2021, 26(3): 271-277.
[8]	杨慧芳, 罗颖, 赵莉. 右美托咪定对脓毒症大鼠肾功能及血清炎症因子的影响[J]. 中国临床药理学与治疗学, 2020, 25(8): 857-861.
[9]	张俊秀, 沈媛媛, 杨解人, 郑书国, 郝伟. 联用芝麻素和维生素E抑制PI3K/AKT信号通路改善自发性高血压大鼠的左室纤维化[J]. 中国临床药理学与治疗学, 2020, 25(11): 1214-1222.
[10]	姜莉，李宇宁，庞立宏，张兴道，王芳. N-乙酰半胱氨酸联合肺表面活性物质对新生儿重症胎粪吸入综合征的疗效及其对胎儿炎症因子及氧合功能的影响[J]. 中国临床药理学与治疗学, 2020, 25(1): 81-86.
[11]	吴加元，郑丽，莫娟芬，郭丽，曹晨曦，鲍轶. N-乙酰半胱氨酸调控非酒精性脂肪性肝病小鼠外周循环及肝脏局部髓源抑制细胞的研究[J]. 中国临床药理学与治疗学, 2019, 24(9): 989-996.
[12]	衡冰冰，戴舒阳，杨丹峰，Beekoo Deepti，连庆泉，上官王宁. 丙泊酚对吗啡鞘内注射所致瘙痒大鼠脊髓内胃泌素释放肽受体的影响[J]. 中国临床药理学与治疗学, 2019, 24(7): 759-765.
[13]	高艺文，张楠，吕婵梅，杨宣梅，岳桂华. 黄连解毒汤对幼龄自发性高血压大鼠血管内皮NO通路影响的研究[J]. 中国临床药理学与治疗学, 2019, 24(3): 248-253.
[14]	赵韶静，曾艳，李正东，张华. 左卡尼汀对大鼠糖尿病肾病的保护作用分析[J]. 中国临床药理学与治疗学, 2019, 24(3): 278-282.
[15]	杜天平，孟祖东. 17β雌二醇对异丙酚诱导发育期大鼠海马神经细胞凋亡时p38MAPK信号传导通路的作用机制[J]. 中国临床药理学与治疗学, 2019, 24(2): 128-133.