十两茶提取物抗高脂诱导家兔动脉粥样硬化形成涉及调节ADMANO通路

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (7): 747-752.

十两茶提取物抗高脂诱导家兔动脉粥样硬化形成涉及调节ADMANO通路

杜万红¹, 刘仲华², 施玲⁴, 周重旺⁵, 彭世喜¹, 张勇¹, 施兆鹏^2,4, 姜德建³

¹解放军163 医院内四科, 长沙 410003, 湖南;
²湖南省天然产物工程技术研究中心, 长沙 410128, 湖南;
³中南大学药学院药理学系, 长沙 410078, 湖南;
⁴湖南农业大学茶学教育部重点实验室, 长沙 410128, 湖南;
⁵湖南省茶业有限公司, 长沙 410005, 湖南

收稿日期:2008-03-11 修回日期:2008-06-20 出版日期:2008-07-26 发布日期:2020-10-14
通讯作者: 刘仲华,男,教授,博士生导师,主要从事茶叶教学及科研工作。Tel:0731-4635306 E-mail:larkin-liu@163.com
作者简介:杜万红,男,在读博士生,主任医师,主要从事心血管疾病的防治研究。
基金资助:
湖南省科技计划重大专项(05NK1004)

Involvement of ADMA NO pathway in antiatherosclerotic properties of the extract of shi-liang cha in hypercholesterolemic diet-fed rabbits

DU Wan-hong¹, LIU Zhong-hua², SHI Ling⁴, ZHOU Zhong-wang⁵, PENG Shi-xi¹, ZHANG Yong¹, SHI Zhao-peng^2,4, JIANG De-jian³

¹Fourth Department of Internal Medicine, 163 Hospital of PLA, Changsha 410003, Hunan, China;
²Hunan Engineering and Technology Center for Natural Products, Changsha 410128, Hunan, China;
³School of Pharmaceutical Sciences, Central South University, Changsha 410013, Hunan, China;
⁴Key Laboratory of Tea Science of Ministry of Education, Changsha 410128, Hunan, China;
⁵Hunan Tea Company Limited, Changsha 410005, Hunan, China

Received:2008-03-11 Revised:2008-06-20 Online:2008-07-26 Published:2020-10-14

摘要/Abstract

摘要： 目的:观察十两茶提取物对高脂诱导家兔动脉粥样硬化的影响。方法:家兔高脂饲料喂养4周后,分别灌胃给予不同剂量的十两茶提取物(25、50和100mg/kg),共4周。实验结束后,颈动脉取血检测红细胞变形能力,测定血脂、NO、非对称二甲基精氨酸(ADMA)和丙二醛(MDA)含量;分离胸主动脉检测脂质斑块面积和血管内皮依赖性舒张功能。结果:十两茶提取物能剂量依赖性地降低高脂饲养家兔血清总胆固醇和低密度脂蛋白水平,同时显著减少主动脉粥样斑块面积和改善血管内皮舒张功能及红细胞变形能力。十两茶提取物显著降低血清MDA和ADMA含量和增加NO水平,且呈剂量依赖性。结论:十两茶提取物具有抗动脉粥样硬化作用,其机制与抑制脂质过氧化、调节ADMANO系统,改善血管内皮功能和红细胞变形能力有关。

关键词: 十两茶, 动脉粥样硬化, 内皮, 非对称二甲基精氨酸, 脂质过氧化

Abstract: AIM: To investigate the effects of the extract of shi-liang cha on atherogenesis in hypercholesterolemic diet-fed rabbits.METHODS: Male rabbits were fed with food containing cholesterol for 8 weeks to induce atherosclerosis.For drug treatment, the animals were orally treated with different doses of the extract of shi-liang cha (25, 50 or 100 mg/kg per day) during the last four-week period of the experiment.At the end of the experiment, blood samples were collected to measure the plasma levels of nitric oxide (NO), asymmetric dimethylarginine (ADMA) and malondialdohyde (MDA), and the aortas were isolated to determine endothelium-dependent vasodilator response and lesion area.RESULTS: The extract of shiliang cha could dose-dependently decrease plasma levels of total cholesterol, low-density lipoprotein and lesion area in atherosclerotic rabbits.Moreover, such treatment could improve endothelium-dependent vasodilator response and erythrocyte deformability, decrease the plasma levels of ADMA and MDA and increase NO content.CONCLUSION: The extract of shi-liang cha lowers blood lipids and inhibits atherogenesis, which may be related to improving endothelial function and erythrocyte deformability via inhibiting lipid peroxidation and modulating ADMA/NO system.

Key words: shi-liang cha, atherosclerosis, endothelium, asymmetric dimethylarginine, lipid peroxidation

中图分类号:

R965.2

杜万红, 刘仲华, 施玲, 周重旺, 彭世喜, 张勇, 施兆鹏, 姜德建. 十两茶提取物抗高脂诱导家兔动脉粥样硬化形成涉及调节ADMANO通路[J]. 中国临床药理学与治疗学, 2008, 13(7): 747-752.

DU Wan-hong, LIU Zhong-hua, SHI Ling, ZHOU Zhong-wang, PENG Shi-xi, ZHANG Yong, SHI Zhao-peng, JIANG De-jian. Involvement of ADMA NO pathway in antiatherosclerotic properties of the extract of shi-liang cha in hypercholesterolemic diet-fed rabbits[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(7): 747-752.

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