染料木素临床不良反应与CYP1A2、UGT1A7基因多态性研究

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (7): 803-808.

染料木素临床不良反应与CYP1A2、UGT1A7基因多态性研究

杨明¹, 唐波², 陈经宝¹, 张娴², 朱首伦¹, 丁春燕¹, 卢运田¹, 曾星²

¹广州中医药大学第二附属医院外三科, ²中心实验室, 广州 510120, 广东

收稿日期:2007-12-12 修回日期:2008-05-04 出版日期:2008-07-26 发布日期:2020-10-14
通讯作者: 曾星,女,研究员,博士研究生导师,研究方向:临床药理学。Tel:020-81887233-30908 E-mail:X-Zeng@21cn.com
作者简介:杨明,男,副主任医师,硕士研究生导师,研究方向:中西医结合泌尿外科及男科。Tel:020-81499399-8123 E-mail:ym198773@126.com
基金资助:
广东省自然科学基金课题(7004840)

Study on genistein clinical adverse reaction and gene polymorphism of CYP1A2、UGT1A7

YANG Ming¹, TANG Bo², CHEN Jing-bao¹, ZHANG Xian², ZHU Shou-lun¹, DING Chun-yan¹, LU Yun-tian¹, ZENG Xing²

¹Third Surgery Department, ²Center Laboratory of the Second Affiliated Hospital, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510120, Guangdong, China

Received:2007-12-12 Revised:2008-05-04 Online:2008-07-26 Published:2020-10-14

摘要/Abstract

摘要： 目的:探讨染料木素临床不良反应与CYP1A2、UGT1A7基因多态性的相关性。方法:114例健康志愿者随机分为试验组与对照组,试验组分别口服染料木素一个剂量50、100、200mg,每人服药1次,观察3d了解有无不良反应发生;用限制性片段长度多态性聚合酶链反应(RFLRPCR)扩增基因片段并酶切电泳观察分析CYP1A2G2964A、C734A和UGT1A7Trp208Arg的多态性。结果:试验组及对照组的基因型及等位基因分布差异无统计学意义(P>0.05)。试验组受试者根据有无不良反应的出现分为两组基因,CYP1A2G2964A基因:不良反应组14例受试者中有10例的基因型为G/A(占71.43%),而无不良反应组41例受试者中有22例的基因型为G/G(占53.66%);CYP1A2C734A基因:不良反应组13例受试者中有7例的基因型为C/A(占53.85%),而无不良反应组32例受试者中有16例的基因型为A/A(占50.00%);UGT1A7Trp208Arg基因:不良反应组15例受试者中有12例的基因型为Trp/Trp(占80.00%),并且无不良反应组53例受试者中有32例的基因型也为Trp/Trp(占60.38%)。结论:染料木素不良反应组中CYP1A2G2964A基因以G/A型较高,CYP1A2C734A基因以G/G型较高;UGT1A7基因以TrpTrp型较高。

关键词: 染料木素, 不良反应, CYP1A2、UGT1A7基因多态性

Abstract: AIM: In order to discuss the relation between clinical adverse reaction and gene polymorphism of CYP1A2、UGT1A7 when using genistein. METHODS: 114 cases of healthy volunteers were randomly divided into 2 groups: model group, and normal group.the model group take genistein 50, 100, 200 mg by oral, and each dose of genistein was observed 3 days for the occurrence of adverse reaction.Gene fragment was amplified by restriction fragment length polymorphism polymerase chain reaction(RFLR-PCR), and polymorphism of CYP1A2G2964A, C734A and UGT1A7Trp208Argwas analysised by enzyme electrophoresis.RESULTS: There had no significant difference of gene distribution between model and normal groups(P>0.05).Model group was divided into 2 groups of genotypes according to adverse reaction occurrence, CYP1A2G2964A gene: the 10 cases genotype among 14 cases in adverse reaction group was G/A(account 71.43%), but 22 cases genotype among 41 cases in no adverse reaction was G/G (account 53.66%); CYP1A2C734A gene: 7 cases genotype among 13 cases in adverse reaction was C/A(account 53.85%), but 16 cases genotype among 32 cases in no adverse reaction was A/A(account 50.00%).UGT1A7Trp 208Arg Gene: 12 cases genotype among 15 cases in adverse reaction was Trp/Trp (account 80.00%), and 32 cases genotype among 53 cases in no adverse reaction was Trp/Trp (account 60.38%).CONCLUSION: The relation gene in adverse reaction by using genistein is CYP1A2G2964A, CYP1A2C734A and UGT1A7, especially higher in G/A, C/A and Trp/Trp for each gene.

Key words: genistein, adverse reaction, CYP1A2, UGT1A7gene polymorphism

中图分类号:

R969

杨明, 唐波, 陈经宝, 张娴, 朱首伦, 丁春燕, 卢运田, 曾星. 染料木素临床不良反应与CYP1A2、UGT1A7基因多态性研究[J]. 中国临床药理学与治疗学, 2008, 13(7): 803-808.

YANG Ming, TANG Bo, CHEN Jing-bao, ZHANG Xian, ZHU Shou-lun, DING Chun-yan, LU Yun-tian, ZENG Xing. Study on genistein clinical adverse reaction and gene polymorphism of CYP1A2、UGT1A7[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(7): 803-808.

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