双氢青蒿素对小鼠Lewis肺癌移植瘤生长的影响

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (8): 891-894.

双氢青蒿素对小鼠Lewis肺癌移植瘤生长的影响

蒋永新, 王前, 陈晓群, 金从国, 李佳, 邓晶

昆明医学院第三附属医院、云南省肿瘤医院肿瘤研究所, 昆明650118, 云南

收稿日期:2008-05-10 修回日期:2008-06-25 出版日期:2008-08-26 发布日期:2020-10-12
作者简介:蒋永新, 女, 硕士, 副教授, 硕士研究生导师, 主要从事抗肿瘤药物研究。Tel:0871-8187257　E-mail:jyongx99@126.com

Effect of Dihydroarteannuin on transplantation tumor growth in C57BL/6J mice with lewis lung cancer

JIANG Yong-xin, WANG Qian, CHEN Xiao-qun, JIN Cong-guo, LI Jia, DENG Jing

Department of Tumor Institue, Third Affiliated Hospital of Kunming Medical College (Yunnan Tumor Hospital), Kunming 650118, Yunnan, China

Received:2008-05-10 Revised:2008-06-25 Online:2008-08-26 Published:2020-10-12

摘要/Abstract

摘要： 目的:探讨双氢青蒿素对小鼠Lewis 肺癌移植瘤的抑瘤效应及其作用机制。方法:50只C57BL/6J 小鼠皮下接种3LL 细胞(2×10⁶), 随机分为5组, 分别为生理盐水组, 阳性对照顺铂组,双氢青蒿素高、中、低剂量组(150、100、50 mg/kg),检测各组小鼠的体重变化和抑瘤率, 应用流式细胞术进行瘤细胞的DNA 倍体分析。结果:双氢青蒿素中、高剂量组体重无生理盐水组增加明显, 其抑瘤率分别为53.50%及59.24%;流式细胞术检测双氢青蒿素能诱导肿瘤细胞凋亡, 并能影响肿瘤的细胞周期, G0G 期及G₂/M 期细胞数大量减少, 细胞被阻滞在S1期。结论:口服双氢青蒿素对Lewis 小鼠肺癌有明显的抑制作用, 可促进肿瘤细胞凋亡。

关键词: 双氢青蒿素, C57BL/6J 小鼠, 肺肿瘤, 凋亡, 细胞周期

Abstract: AIM:To explore the anti-tumor effect and the mechanism of dihydroarteannuin in C57BL/6J mice with lewis lung cancer.METHODS:Fifty C57BL/6J mice subcutaneously planted with 3LL lung cancer cells (2×10⁶)were randomly divided into 5 groups :blank control group, same volume of normal saline group, positive control DDP group, low, middle and high dose dihydroarteannuin groups.Changes of body weight and inhibitory rate of tumor in each group were observed.The cell cycle and apoptosis rates were analyzed by flow cytometry.RESULTS:The body weights were decreased in middle and high dose group compared with NS group and the inhibitory rate of tumor were 53.50% and 59.24% respectively.FCM assay indicated that Dihydroarteannuin could induce apoptosis of lung cancer cell.At the same time, the number of G0G1 and G₂/M phase cells was decreased. Most of the cells were arrested in S1phase.CONCLUSION:Dihydroarteannuin has obviously effect on anti-tumor in C57BL/6J mice with lung cancer.Its possible mechanism might be involved in inducing cancer cell apoptosis.

Key words: Dihydroarteannuin, C57BL/6J mice, lung cancer, apoptosis, cell cycle

中图分类号:

R965.2

蒋永新, 王前, 陈晓群, 金从国, 李佳, 邓晶. 双氢青蒿素对小鼠Lewis肺癌移植瘤生长的影响[J]. 中国临床药理学与治疗学, 2008, 13(8): 891-894.

JIANG Yong-xin, WANG Qian, CHEN Xiao-qun, JIN Cong-guo, LI Jia, DENG Jing. Effect of Dihydroarteannuin on transplantation tumor growth in C57BL/6J mice with lewis lung cancer[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(8): 891-894.

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