黄芪注射液对哮喘大鼠p38蛋白激酶和白细胞介素-5表达的影响

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (8): 900-904.

黄芪注射液对哮喘大鼠p38蛋白激酶和白细胞介素-5表达的影响

黄翠萍, 杨和平, 杨颖乔

咸宁学院医学院内科, 咸宁437100, 湖北

收稿日期:2008-06-03 修回日期:2008-07-12 出版日期:2008-08-26 发布日期:2020-10-12
作者简介:黄翠萍, 女, 医学博士, 副教授, 主要从事慢性阻塞性肺疾病的研究。Tel:0715-8268279　E-mail:huangcuiping168@126.com
基金资助:
湖北省科技厅科研项目(2006ABA330)

Protective effects and mechanism of astragalus injection on asthmatic rats

HUANG Cui-ping, YANG He-ping, YANG Ying-qiao

Department of Respiratory Medicine, the First Affiliated Hospital of Xianning Medical College, Xianning 437100, Hubei, China

Received:2008-06-03 Revised:2008-07-12 Online:2008-08-26 Published:2020-10-12

摘要/Abstract

摘要： 目的:探讨黄芪注射液对哮喘大鼠p38蛋白激酶(p38 MAPK)和白细胞介素-5(IL-5)表达的影响。方法:应用鸡卵清蛋白(OVA)腹腔注射致敏和反复超声雾化吸入复制大鼠哮喘模型。40只大鼠随机分成5组:正常对照组, 哮喘模型组和黄芪注射液低、中、高剂量组(2.5、5.0、10.0 mL/kg)。分别采用酶联免疫吸附法(ELISA)、原位分子杂交方法和蛋白质印迹检测支气管肺泡灌洗液(BALF)IL-5含量、肺组织IL-5 mRNA和磷酸化p38 MAPK 表达的变化, 并观察BALF 中炎症细胞计数、分类以及肺组织病理学变化。结果:哮喘模型组大鼠BALF 中炎症细胞计数、IL-5含量和肺组织中IL-5 mRNA及磷酸化p38 MAPK 表达均较正常对照组显著增加(P<0.01);黄芪干预组的上述改变较哮喘模型组显著降低(P<0.01), 肺组织病理学损伤程度明显减轻, 黄芪注射液低、中、高剂量组之间差异无统计学意义。肺组织磷酸化p38 MAPK 表达水平与BALF中嗜酸性粒细胞(EOS)计数和IL-5、IL-5 mRNA含量之间分别呈显著正相关(r=0.62、0.69、0.74, P<0.01)。结论:p38 MAPK 可能参与了支气管哮喘的发病过程。黄芪对哮喘的治疗作用可能部分与抑制p38 MAPK 的磷酸化活化、降低炎症介质释放、减轻炎症细胞浸润有关。

关键词: 支气管哮喘, 丝裂原活化蛋白激酶, 黄芪, 炎症介质, 气道炎症

Abstract: AIM:To explore the protective effects and mechanism of astragalus injection on asthmatic rats.METHODS:OVA was injected intraperitoneally and inhaled to produce the asthmatic rat models.Forty rats were randomly divided into five groups :control group, asthma group and astragalus groups of high, medium and low dose.The concentration of IL-5 in BALF and the expression of IL-5 mRNA, phospho-p38 MAPK in lung tissue were respectively measured by ELISA, in situ hybridization staining and Western blot.The number of inflammatory cell in BALF was calculated and histopathology changes were observed. RESULTS:The number of inflammatory cell and the concentration of IL-5 in BALF and the expression of IL-5 mRNA, phospho-p38 MAPK in lung tissue were higher in asthmatic rat group than those in normal control(P<0.01);and those in astragalus rat group were lower than in asthmatic rat group(P<0.01).The histopathology damage was alleviated in astragalus rat group.The efficacies in the astragalus groups of high, medium and low dose were similar, showing no insignificant difference among them.The expression of phospho-p38 MAPK has positive correlation with the number of eosinophil and the concentration of IL-5, the 中国临床药理学与治疗学 2008 Aug;13(8)· 903· expression of IL-5 mRNA(r=0.62, 0.69, 0.74, P<0.01).CONCLUSION:p38 MAPK may play a role in pathological process of asthma.Astragalus could effectively treat asthma by inhibiting the expression of phospho-p38 MAPK and IL-5, and decreasing the number of inflammatory cells

Key words: asthma, mitogen-activated protein kinase, astragalus, airway inflammation, cytokine

中图分类号:

R965.2

黄翠萍, 杨和平, 杨颖乔. 黄芪注射液对哮喘大鼠p38蛋白激酶和白细胞介素-5表达的影响[J]. 中国临床药理学与治疗学, 2008, 13(8): 900-904.

HUANG Cui-ping, YANG He-ping, YANG Ying-qiao. Protective effects and mechanism of astragalus injection on asthmatic rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(8): 900-904.

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