皖南尖吻蝮蛇蛇毒中抗肿瘤活性蛋白分离纯化及其活体外性研究

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (9): 991-995.

皖南尖吻蝮蛇蛇毒中抗肿瘤活性蛋白分离纯化及其活体外性研究

潘学兵, 陈冬云, 怀建国, 吉兆宁

皖南医学院弋矶山医院肿瘤防治中心, 芜湖241001, 安徽

收稿日期:2008-07-14 修回日期:2008-09-11 出版日期:2008-09-26 发布日期:2020-10-13
通讯作者: 吉兆宁, 男, 博士, 教授, 主任医师, 硕士生导师, 研究方向:肿瘤化学及生物防治机理和临床应用。Tel:0553-5739311 E-mail:jzning@163.com
作者简介:潘学兵, 男, 主治医师, 硕士研究生, 研究方向:肿瘤化学及生物防治机理和临床应用。

Purification of antineoplasmic activity protein from the venom of Wannan Agkistrodon and the study in vitro

PAN Xue-bing, CHEN Dong-yun, HUAI Jian-guo, JI Zhao-ning

Treatment and Prevention of Tumour Center, Yijishan Hospital of Wannan Medical College, Wuhu 241001, Anhui,China

Received:2008-07-14 Revised:2008-09-11 Online:2008-09-26 Published:2020-10-13

摘要/Abstract

摘要： 目的:分离纯化皖南尖吻蝮蛇蛇毒(WannanAgkistrodon acutus venom) 中抗肿瘤活性蛋白并研究其活性。方法:利用DEAE-sepharose Fast Flow和SP-sepharose Fast Flow 阴阳离子交换层析以及Sephadex G-75 凝胶过滤层析等分离方法从皖南尖吻蝮蛇蛇毒中分离纯化得一种抗肿瘤活性蛋白,用CCK-8 法检测该蛋白对体外培养的白血病K562 细胞、结肠癌细胞(SW480)、胃癌细胞(SGC7901)、肝癌细胞(HepG2) 的增殖抑制作用。结果:分离纯化得一相对分子质量约23700 的抗肿瘤活性组分(ATF1-c), CCK-8 检测对体外培养的K562、SW480、SGC7901、HepG2 细胞的增殖抑制作用, 呈剂量-时间依赖关系。结论:ATF1-c 对体外培养的人癌细胞有明显的抑制和杀伤作用。

关键词: 尖吻蝮蛇, 分离纯化, CCK-8比色法, K562, SW480, SGC7901, HepG2

Abstract: AIM: To purify the antineoplasmic active protein from the venom ofWannan Agkistrodon and to study the activity in vitro.METHODS: An antineoplasmic activity proteinum was isolatded from the venom of Wannan Agkistrodon by DEAE-sepharose Fast Flow and SP-sepharose Fast Flow column chromatography and Sephadex G-75 gel filtration chromatography, the generation depressant effects of this proteinum were detected by CCK-8 method in some tumor cells such as K562, SW480, SGC7901, HepG2 were cultured in vitro.RESULTS: An antineoplasmic activity ingredient named ATF1-c was discovered, its molecular weight was 23700.The generation depressant effects of K562, SW480, SGC7901, HepG2 cells cultured in vitro were detected by CCK-8 method, and there were dosage-time dependent relationships.CONCLUSION: ATF1-c has significant inhibited and lethal effect to human cancer cells cultured in vitro.

Key words: Agkistrodon, Separationandpurification, CCK-8colorimetry, K562, SW480, SGC7901, HepG2

中图分类号:

R965.2

潘学兵, 陈冬云, 怀建国, 吉兆宁. 皖南尖吻蝮蛇蛇毒中抗肿瘤活性蛋白分离纯化及其活体外性研究[J]. 中国临床药理学与治疗学, 2008, 13(9): 991-995.

PAN Xue-bing, CHEN Dong-yun, HUAI Jian-guo, JI Zhao-ning. Purification of antineoplasmic activity protein from the venom of Wannan Agkistrodon and the study in vitro[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(9): 991-995.

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