尼莫地平抑制缺血再灌注后成年大鼠脑内神经发生

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (1): 32-36.

尼莫地平抑制缺血再灌注后成年大鼠脑内神经发生

田和平, 顾斌, 程峰, 郝怀勇, 胡卫星, 李立新

南京医科大学第一附属医院神经外科, 南京 210029, 江苏

收稿日期:2008-11-04 修回日期:2009-01-07 出版日期:2009-01-26 发布日期:2020-10-27
作者简介:田和平, 男, 硕士, 研究方向:中枢神经损伤与修复的基础研究。Tel:13776602750 E-mail:grantnw@163.com
基金资助:
江苏省“ 兴卫工程” 医学重点人才项目(RC2007062)

Nimodipine inhibits ischemia-reperfusion triggered neurogenesis in adult rats

TIAN He-ping, GU Bin, CHENG Feng, HAO Huai-yong, HU Wei-xin, LI Li-xin

Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu, China

Received:2008-11-04 Revised:2009-01-07 Online:2009-01-26 Published:2020-10-27

摘要/Abstract

摘要： 目的: 观察尼莫地平(Nimodipine) 对大鼠脑缺血再灌注后海马齿状回神经发生的影响并探讨其相关机制。方法: 采用四动脉阻断法诱导大鼠全脑缺血, 缺血20 min 前腹腔内注射尼莫地平或脑室内注射细胞外信号调节激酶(extracellular signal-regulated kinase, ERK) 的抑制剂U0126;免疫组化Brdu 标记法检测脑内海马神经发生;WesternBlot 法检测海马组织ERK 、磷酸化ERK(p-ERK) 蛋白的表达。结果: 尼莫地平抑制脑缺血再灌注后海马部位的神经发生的同时也抑制了脑缺血再灌注后海马齿状回p-ERK 的表达;海马部位神经发生在U0126 组与U0126+尼莫地平联合给药组差异无统计学意义。结论: 尼莫地平显著抑制脑缺血再灌注后海马齿状回的神经发生, 其机制与下调p-ERK 表达密切相关。

关键词: 脑缺血, 神经发生, 尼莫地平, 细胞外信号调节激酶

Abstract: AIM: To observe the effects of Nimodipine on hippocampal dentate gyrus neurogenesis of the adult rat triggered by ischemia-reperfusion and investigate its molecular mechanism.METHODS: The cerebral ischemia-reperfusion models were established by four-vessel-blocked method.Nimodipine or the extracellular signal-regulated kinase inhibitor U0126 was administrated by intravenous injection or intraventricular injection, respectively before ischemia 20 min.The hippocampal dentate gyrus neurogenesis was detected by immunohistochemistry of 5-bromodeoxyuridine (Brdu).The expressions of ERK and p-ERK were detected by Western blotting.RESULTS: Nimodipine not only inhibited hippocampal dentate gyrus neurogenesis but also the expression of p-ERK in the area of dentate gyrus after ischemia-reperfusion.There was no significant difference in hippocampal dentate gyrus neurogenesis between the U0126 group and U0126 combined with Nimodipine group.CONCLUSION: Nimodipine inhibits hippocampal dentate gyrus neurogenesis, and it may be related with the down-regulation p-ERK after ischemia-reperfusion.

Key words: cerebral ischemia-reperfusion, neurogenesis, Nimodipine, extracellular signal-regulated kinase

中图分类号:

R966

田和平, 顾斌, 程峰, 郝怀勇, 胡卫星, 李立新. 尼莫地平抑制缺血再灌注后成年大鼠脑内神经发生[J]. 中国临床药理学与治疗学, 2009, 14(1): 32-36.

TIAN He-ping, GU Bin, CHENG Feng, HAO Huai-yong, HU Wei-xin, LI Li-xin. Nimodipine inhibits ischemia-reperfusion triggered neurogenesis in adult rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(1): 32-36.

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