雷公藤内酯醇对大鼠重症急性胰腺炎肺损伤的保护作用

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (1): 57-61.

雷公藤内酯醇对大鼠重症急性胰腺炎肺损伤的保护作用

李兴旺¹, 尚游², 张冰¹, 胡明品¹, 连庆泉¹

¹温州医学院附属第二医院麻醉科, 温州 325027, 浙江;
²华中科技大学同济医学院附属协和医院麻醉科, 武汉 430022, 湖北

收稿日期:2008-10-06 修回日期:2008-12-22 出版日期:2009-01-26 发布日期:2020-10-27
基金资助:
温州市科技局立项课题( Y2005A144)

Protective effect of triptolide against severe acute pancreatitis-associated lung injury in rats

LI Xing-wang¹, SHANG You², ZHANG Bing¹, HU Ming-pin¹, LIAN Qing-quan¹

¹Department of Anesthesiology, Second Affiliated Hospital of Wenzhou Medical College, Wenzhou 325027, Zhejiang, China;
²Department of Anesthesiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, China

Received:2008-10-06 Revised:2008-12-22 Online:2009-01-26 Published:2020-10-27

摘要/Abstract

摘要： 目的: 研究雷公藤内酯醇对重症急性胰腺炎肺损伤的保护作用及其可能作用机制。方法: 36 只雄性Wistar 大鼠, 随机分为3 组, 每组12 只:假手术组(Sham 组) 、模型组(ALI 组) 和雷公藤内酯醇处理组(Tri 组) 。Sham 组只行开腹术,ALI 组和Tri 组经胆胰管给予牛磺胆酸钠后, 分别腹腔注射生理盐水、雷公藤内酯醇0.2 mg/kg 。注射牛磺胆酸钠后6 h 处死动物。每组中6 只大鼠在处死前15 min 静脉注射伊文思蓝(EB) 20 mg/kg 。测定支气管肺泡灌洗液(BALF) 中髓过氧化物酶(MPO) 活性和中性粒细胞(PMN) 数。检测肺组织湿干重比(W/D) 、EB 含量、MPO 活性、肺组织细胞间黏附分子1 (ICAM-1) 及PMN CD_11b/CD₁₈ 表达。观察肺组织病理学的改变。结果: 与Sham 组比较, ALI 组肺组织病理学损伤严重, BALF 中MPO 、PMN 水平和肺组织W/D 、EB 及MPO 水平、ICAM-1及PMN CD_11b/CD₁₈ 表达水平升高(P<0.01);与ALI 组比较, Tri 组上述指标均降低(P<0.05 或0.01) 。结论: 雷公藤内酯醇对重症急性胰腺炎肺损伤具有一定保护作用, 与下调ICAM-1 及PMNCD_11b/CD₁₈的表达进而抑制PMN 在肺组织的聚集、激活有关。

关键词: 雷公藤内酯醇, 重症急性胰腺炎, 呼吸窘迫综合征, 成人

Abstract: AIM: To investigate the effects of triptolide on severe acute pancreatitis-associated lung injury in rats and assess the possible mechanisms. METHODS: Thirty-six male Wistar rats were randomly divided into 3 groups (n=12):Sham, model and triptolide group.Sham group only underwent laparotomy.Immediately after undergoing retrograde intraductal injection of sodium taurocholate, triptolide and model groups received triptolide 0.2 mg/kg and normal saline, respectively.Six hours after sodium taurocholate injection, rats were sacrificed.Six animals in each group were intravenously injected with Evans blue (EB) 20 mg/kg at 15 min before sacrifice.Bronchoalveolar lavage fluid (BALF) was collected for determination of PMN count andMPO activity.The lungs were removed for evaluation of histological injury and determination of wet dry lung weight (W/D) ratio, EB content, myeloperoxidase (MPO) activity, and expression levels of ICAM-1.The blood was obtained for determining the expression levels of PMN CD_11b/CD₁₈.The histopathologic changes of lung were observed.RESULTS: Compared with sham group, the histopathologic injury of lung in model group was serious, and the MPO activity, PMN count in BALF, the W/D ratio, EB content, MPO activity, the expression of ICAM-1 and PMN CD_11b/CD₁₈ in lung tissues were increased.Compared with model group, all of these changes were significantly reduced in triptolide group (P<0.05 or 0.01).CONCLUSION: Triptolide has protective effect against severe acute pancreatitis-associated lung injury in rats.The underlying mechanisms are via an inhibition of neutrophilic recruitment and activity through down-regulating the expressions of lung ICAM-1 and PMN CD_11b/CD₁₈.

Key words: triptolide, pancreatitis, respiratory distress syndrome, adult

中图分类号:

R965.1

李兴旺, 尚游, 张冰, 胡明品, 连庆泉. 雷公藤内酯醇对大鼠重症急性胰腺炎肺损伤的保护作用[J]. 中国临床药理学与治疗学, 2009, 14(1): 57-61.

LI Xing-wang, SHANG You, ZHANG Bing, HU Ming-pin, LIAN Qing-quan. Protective effect of triptolide against severe acute pancreatitis-associated lung injury in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(1): 57-61.

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