诱导型一氧化氮合酶在哮喘大鼠肺组织及中性粒细胞中的表达及地塞米松对其的影响

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (10): 1128-1132.

诱导型一氧化氮合酶在哮喘大鼠肺组织及中性粒细胞中的表达及地塞米松对其的影响

陈存国¹, 金海华¹, 李绍波¹, 金小红¹, 陈保国¹, 童夏生², 叶辉³

¹温州医学院附属台州医院, 临海 317000, 浙江;
²浙江省台州中西医结合医院, 温岭 317523, 浙江;
³台州市第一人民医院, 台州 318020, 浙江

收稿日期:2009-06-30 修回日期:2009-09-16 发布日期:2020-10-29
通讯作者: 童夏生,男,硕士,副主任医师,研究方向:呼吸与哮喘。Tel:13758688128 E-mail:xshtzg@163.com
作者简介:陈存国,男,副主任医师,研究方向:呼吸系统疾病。Tel:13606656798 E-mail:jinxhtz @163.com
基金资助:
浙江省卫生厅资助项目(2007-B238);温岭市科技局基金资助项目(2006-53)

Inducible nitric oxide synthase expression in lung tissue and neutrophil of asthmatic rats and the effects of dexamethasone on them

CHEN Cun-guo¹, JIN Hai-hua¹, LI Shao-bo¹, JIN Xiao-hong¹, CHEN Bao-guo¹, TONG Xia-sheng², YE Hui³

¹Taizhou Hospital Affiliated to Wenzhou Medical College, Linhai 317000, Zhejiang, China;
2Taizhou Integrated Traditional and Western Medicine Hospital, Wenling 317523, Zhejiang, China;
3Taizhou First People' s Hospital, Taizhou 318020, Zhejiang, China

Received:2009-06-30 Revised:2009-09-16 Published:2020-10-29

摘要/Abstract

摘要： 目的观察诱导型一氧化氮合酶(iNOS)在哮喘大鼠肺组织及血中性粒细胞(PMN)中的表达, 探讨PMN 参与哮喘炎症的可能作用机制。方法采用大鼠哮喘模型, 随机分成哮喘组、对照组、地塞米松干预组, 分离纯化血PMN, 免疫组织化学法检测iNOS 蛋白的表达水平, 测定支气管肺泡灌洗液(BALF)中NO 浓度。结果哮喘组PMNiNOS 蛋白光密度(OD)值(0.122 ±0.017)的表达水平显著高于对照组OD 值(0.076 ±0.014)(P <0.01), 地塞米松干预组OD 值(0.089 ±0.013)PMN iNOS 蛋白的表达水平显著低于哮喘组OD值(P <0.01), 但与对照组相比差异无统计学意义。哮喘组支气管壁iNOS 蛋白OD 值(0.243 ±0.039)的表达水平显著高于对照组(0.119 ±0.016)(P <0.01), 地塞米松干预组OD 值(0.164±0.016)支气管壁iNOS 蛋白的表达水平显著低于哮喘组且高于对照组(P 均<0.01)。哮喘组BALF NO 浓度(8.59 ±1.07)ng/mL显著高于对照组(3.69 ±1.00)ng/mL (P <0.01), 地塞米松干预组BALF NO 浓度(4.28 ±0.89)ng/mL显著低于哮喘组(P <0.01), 但与对照组相比差异无统计学意义。PMN iNOS 蛋白的表达水平与BALF NO 浓度呈显著正相关(n =29, r =0.770, P <0.01)。支气管壁iNOS 蛋白的表达水平与BALF NO 浓度呈显著正相关(n =29, r =0.802, P <0.01)。结论哮喘大鼠PMN 合成iNOS 蛋白的功能增加,PMN 可能通过iNOS 参与哮喘的发病机制, 这种功能可以部分被地塞米松所抑制。

关键词: 哮喘, 诱导型一氧化氮合酶, 一氧化氮, 中性粒细胞, 大鼠

Abstract: AIM: To observe the expression of inducible nitric oxide synthase (iNOS)in neutrophils and lung tissues and explore the roles of peripheral blood neutrophils in the pathogenesis of asthmatic inflammation in asthmatic rats. METHODS: Experimental rats were randomly divided into asthma, control and dexamethasone treated groups.Peripheral blood neutrophils were isolated and the expression of iNOS was detected by immunohistochemistry.The nitric oxide (NO)concentration of bronchoalveolar lavage fluids (BALF)was also determined. RESULTS: iNOS protein of PMN in asthmatic rats (0.122 ±0.017 Optical density)was significantly higher than that in control group (0.076 ±0.014, P <0.01).iNOS protein of PMN in dexamethasone treated group (0.089 ±0.013)was markedly lower than that in asthma group (P <0.01), but there was no statistically significant for the PMN iNOS expression between the dexamethasone treated group and control group.Furthermore, iNOS of bronchial wall in asthma group (0.243 ±0.039)was dramatically higher than that in control group (0.119 ±0.016, P <0.01).iNOS in dexamethasone treated group (0.164 ±0.016)was significantly lower than that in asthma group, but was higher than that in control group (P <0.01, respectively).NO concentration of BALF in asthma group (8.59 ±1.07)ng/mL was significantly higher than control group (3.69 ±1.00 ng/mL, P <0.01).NO concentration of BALF in dexamethasone treated group (4.28 ±0.89)ng/mL was significantly lower than that in asthma group, while no significance was observed between the indexamethasone treated and control group.Moreover, iNOS protein expression of PMN was highly correlated with NO concentration of BALF (n =29, r =0.770, P <0.01), and a similar strong association was established between the bronchial wall iNOS and NO concentration of BALF (n =29, r =0.802,P <0.01). CONCLUSION: iNOS of PMN was elevated in asthmatic rats, which could be partly inhibited by dexamethasone.Our study indicated that an increased iNOS expression of PMN may involved in the pathogenesis of asthma in rats.

Key words: asthma, inducible nitric oxide synthase, nitric oxide, peripheral blood monocytes, rat

中图分类号:

R965.2

陈存国, 金海华, 李绍波, 金小红, 陈保国, 童夏生, 叶辉. 诱导型一氧化氮合酶在哮喘大鼠肺组织及中性粒细胞中的表达及地塞米松对其的影响[J]. 中国临床药理学与治疗学, 2009, 14(10): 1128-1132.

CHEN Cun-guo, JIN Hai-hua, LI Shao-bo, JIN Xiao-hong, CHEN Bao-guo, TONG Xia-sheng, YE Hui. Inducible nitric oxide synthase expression in lung tissue and neutrophil of asthmatic rats and the effects of dexamethasone on them[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(10): 1128-1132.

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