SB203580 对大鼠再灌注损伤肺细胞凋亡的干预

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (11): 1242-1246.

SB203580 对大鼠再灌注损伤肺细胞凋亡的干预

郑艳容¹, 石璐², 洪加林³, 贾旭广², 王万铁²

¹温州市疾病预防控制中心, 温州325027, 浙江;
²温州医学院病理生理学教研室, 温州325035, 浙江;
³永嘉县人民医院, 温州325100, 浙江

收稿日期:2009-07-23 修回日期:2009-11-02 发布日期:2020-10-26
通讯作者: 王万铁,男, 教授, 硕士生导师, 主要从事脏器缺血/再灌注损伤的机制及防治研究。Tel:0577-86689817 E-mail:wzwwt@tom.com
作者简介:郑艳容, 女, 主管护师, 主要从事脏器缺血再灌注损伤研究。Tel:0577-86689817 　E-mail:zjyjhjl@163.com
基金资助:
温州市科技局资助项目(Y20070030)

Effect of SB203580 on cell apoptosis during lung ischemic/reperfusion injury in rats

ZHENG Yan-rong¹, SHI Lu², HONG Jia-lin³, JIA Xu-guang², WANG Wan-tie²

¹Centers for Disease Prevention and Control of Wenzhou, Wenzhou 325000, Zhejiang, China;
²Department of Pathophysiology, Wenzhou Medical College, Wenzhou 325035, Zhejiang, China;
³The People's Hospital of Yongjia,Wenzhou 325100, Zhejiang, China

Received:2009-07-23 Revised:2009-11-02 Published:2020-10-26

摘要/Abstract

摘要： 目的：探讨P38 丝裂原活化蛋白激酶(p38MAPK) 特异性抑制剂SB203580 对再灌注损伤肺细胞凋亡的影响。方法：30 只SD 大鼠随机分为3 组:假手术对照(control, C) 组, 肺缺血/再灌注(I/R) 组, 肺缺血/再灌注+SB203580(S 组);TUNEL 法检测肺组织细胞凋亡的改变;免疫组化法检测Bcl-2 、Bax 蛋白的表达;电镜观察肺组织形态学改变。结果：I/R 组与C 组比较, Bcl-2 蛋白和Bax 蛋白表达均明显上调(均P <0.01), Bcl-2Bax 比值显著下降(P <0.01), 凋亡指数(AI) 显著增高(P <0.01), 肺组织超微结构明显异常;使用SB203580 后, Bcl-2 蛋白表达上调(P <0.01), Bax蛋白表达下调(P <0.01), Bcl-2/Bax 比值上升(P<0.01),AI 显著下降(P <0.01), 肺组织超微结构异常改变不同程度减轻。结论：SB203580 可通过上调Bcl-2 蛋白的表达、下调Bax 蛋白的表达、调控Bcl-2/Bax 蛋白之间的平衡而减轻细胞凋亡,对肺缺血/再灌注损伤发挥积极的防治作用。

关键词: 肺, 再灌注损伤, SB203580, P38 丝裂原活化蛋白激酶, 细胞凋亡

Abstract: AIM: To explore the effect of SB203580, P38 mitogen activated protein kinase specific inhibitor(p38MAPK), on apoptosis during lung ischemic/reperfusion injury in rats. METHODS: 30 rats were randomly divided into three groups, group control (Group C ), group lung ischemia/reperfusion injury (Group I/R) and group SB203580 (Group S).The changes of arteriae pulmonalis apoptosis were detected with TdT-mediated dUTP nick end labeling (TUNEL). The content of Bcl-2 and Bax in cytoplasm were determined with immunohistochemistry and meanwhile the ultrastruction changes of lung were observed under electron microscope. RESULTS: Compared with Group C, the expressions of Bcl-2, Bax protein were increased and the ratio of Bcl-2/Bax were decreased in Group I/R (all P <0.01), and the apoptosis index(AI) was increased, the abnormal changes of the lung tissue in morphologically were significant in Group I/R.After using SB203580, the expression of Bcl-2 protein was increased and the expression of Bax protein was decreased, the ratio of Bcl-2/Bax was increased(all P < 0.01), the values of AI was decreased in Group I/R, and the abnormal changes of the lung tissue in morphologically were lessen at different degree (all P < 0.01). CONCLUSION: SB203580 produces notable protective effects on lung ischemic/reperfusion injury in rats by up-regulating Bcl-2 protein expression, downregulating Bax protein expression in lung tissue and regulating the balance of Bcl-2 protein and Bax protein, decreasing apoptosis.

Key words: lung, reperfusion injury, SB203580, p38 mitogen activated protein kinase, apoptosisun

中图分类号:

R363

郑艳容, 石璐, 洪加林, 贾旭广, 王万铁. SB203580 对大鼠再灌注损伤肺细胞凋亡的干预[J]. 中国临床药理学与治疗学, 2009, 14(11): 1242-1246.

ZHENG Yan-rong, SHI Lu, HONG Jia-lin, JIA Xu-guang, WANG Wan-tie. Effect of SB203580 on cell apoptosis during lung ischemic/reperfusion injury in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(11): 1242-1246.

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