五种天然药物抑制晶状体上皮细胞增殖效应比较

摘要/Abstract

摘要： 目的：对五种天然药物姜黄素(curcumine, Cur) 、榄香烯(elemene, Ele) 、三氧化二砷(arsenite trioxide,As₂O₃) 、雷公藤内酯醇(triptolide, Tri) 、莪术(rhizoma curcumae, Rc) 抑制重组人碱性成纤维细胞生长因子(recombinant human basic fibroblast growth factor, rhbFGF) 诱导的牛晶状体上皮细胞(lens epithelial cell, LEC) 增殖效应进行比较。方法：将rhbFGF 诱导体外培养的LEC 发生增殖, 并用Cur 、Ele 、As₂O₃ 、Tri 、Rc 分别与其共同孵育后,用MTT 法检测LEC 的活性;用流式细胞术(flow cytometer, FCM) 检测LEC 增殖细胞核抗原(proliferating cell nuclear antigen, PCNA) 表达。结果：增殖组LEC 活性、PCNA 蛋白表达显著高于对照组(P <0.01);Cur 组、Ele 组、As2O3 组、Tri 组、Rc 组的LEC 活性、PCNA 蛋白表达均比增殖组显著降低(P <0.01) 。其中Cur 、Ele 的作用最强, As2O3的作用次之, Tri 作用较弱, Rc 作用最弱(P <0.01) 。Cur 和Ele 的作用相近(P >0.05) 。结论：五种天然药物Cur 、Ele 、As₂O₃ 、Tri 、Rc 均可抑制LEC 增殖。Cur 和Ele 的作用最佳, 有希望成为防治后发性白内障的有效药物。

关键词: 姜黄素, 榄香烯, 三氧化二砷, 雷公藤内酯醇, 莪术, 晶状体上皮细胞, 碱性成纤维细胞生长因子, 增殖, 后发性白内障

Abstract: AIM: To compare the inhibitory effect of five natural medicines on proliferation induced by recombinant human basic fibroblast growth factor (rhbFGF) in lens epithelial cell (LEC). METHODS: The proliferations of cultured LEC were induced by rhbFGF, which were incubated with five natural medicines, [curcumine (Cur), elemene(Ele), arsenite trioxide (As₂O₃ ), triptolide (Tri) and rhizoma curcumae (Rc) ], respectively.The LEC activities were detected via methyl thiazolyl tetrazolium (MTT).The proliferating cell nuclear antigen (PCNA) of LEC were detected via flow cytometer(FCM). RESULTS: The activity of LEC and the expression of PCNA in proliferation group were higher than those in control group (P <0.01). Compared with the proliferation group, the activity of LEC and the expression of PCNA were significantly decreased in group Cur, Ele, As₂O₃, Tri and Rc (P < 0.01).The inhibitory effects of Cur and Ele are the strongest.As₂O₃, in the middle.Tri and Rc are the weakest(P <0.01), the effects of Cur and Ele are similar. CONCLUSION: The five natural medicines can inhibit the proliferation of LEC induced by rhbFGF.Cur and Ele may become effective medicines of preventing and treating after-cataract.

Key words: curcumine, elemene, arsenite trioxide, triptolide, rhizoma curcumae, lens epithelial cell, recombinant human basic fibroblast growth factor, proliferation, after-cataract

中图分类号:

R965.2

祁明信, 张清林, 黄秀榕, 杨昕. 五种天然药物抑制晶状体上皮细胞增殖效应比较[J]. 中国临床药理学与治疗学, 2009, 14(11): 1258-1262.

QI Ming-xin, ZHANG Qing-lin, HUANG Xiu-rong, YANG Xin. Comparison of inhibitory effect of five natural medicines on proliferation induced by rhbFGF in lens epithelial cell[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(11): 1258-1262.

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