胰岛素抵抗大鼠体内儿茶酚胺含量变化机制研究

摘要/Abstract

摘要： 目的观察由高脂饲料诱导的胰岛素抵抗(IR) 大鼠中枢与外周儿茶酚胺(CA) 含量的变化并探讨其机制。方法用空腹血糖、空腹血胰岛素、胰岛素敏感指数、IR 指数等指标来评估IR 动物模型的形成;用血清皮质酮反映IR 形成过程中糖皮质激素的变化;用套尾法测量血压;用下丘脑、脑干、血清、肾上腺组织中多巴胺(DA) 、肾上腺素(E) 和去甲肾上腺素(NE) 的变化来评价IR大鼠体内中枢与外周CA 交感神经活性的变化。结果高脂饲料喂养8 周后大鼠出现IR 并伴SBP升高, 中枢与外周CA 的含量明显升高(P<0.01) ;给予米非司酮和罗格列酮后血压明显改善, 中枢与外周CA 的含量明显下降(P<0.05)。结论高脂饲料诱发的IR 状态大鼠中枢与外周CA 的升高与血浆中糖皮质激素的改变有关。

关键词: 胰岛素抵抗, 高血压, 儿茶酚胺, 交感神经, 米非司酮

Abstract: AIM: To observe the changes of catecholamine content in the center and periphery of insulin-resistance rats induced by high-fat diet. METHODS: The formation of insulin-resistance model was assessed with serum glucose, serum insulin, index of insulin sensitivity and index of insulin resistance.The role of mifepristone antagonizing glucocorticoid was checked by the change of serum corticosterone.The blood pressure was measured by the tail cuff method. Activities of the catecholamine sympathetic nervous in the center and periphery of insulin-resistance rats were investigated by the measurements of dopamine, adrenaline, noradrenaline in hypothalamus, brainstem, serum and adrenals. RESULTS: Administration of 8 weeks of high-fat diet induced insulin-resistance rats model with increase of systolic blood pressure and catecholamine contents in the center and periphery (P<0.01).Mifepristone significantly improved blood pressure and catecholamine in the center and periphery (P<0.05). CONCLUSION: Increased catecholamine contents in the center and periphery might be related to the changes of glucocorticoid in serum of insulin-resistance rats induced by high-fat diet.

Key words: insulin-resistance, hypertension, catecholamine, sympathetic nervous, mifepristone

中图分类号:

R587

李姗, 丁启龙. 胰岛素抵抗大鼠体内儿茶酚胺含量变化机制研究[J]. 中国临床药理学与治疗学, 2009, 14(3): 250-255.

LI Shan, DING Qi-long. Study on mechanism of changes of catecholamine content in insulin-resistance rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(3): 250-255.

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