布地奈德对哮喘大鼠中性粒细胞诱导型一氧化氮合酶表达的影响

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (9): 1008-1012.

布地奈德对哮喘大鼠中性粒细胞诱导型一氧化氮合酶表达的影响

叶辉¹, 应小明¹, 童夏生², 陈利中¹, 赵蓓¹

¹浙江省台州市第一人民医院儿科, 台州318020, 浙江;
²浙江省台州中西医结合医院儿科, 温岭317523, 浙江

收稿日期:2009-04-24 修回日期:2009-08-11 发布日期:2020-11-03
作者简介:叶辉, 男, 硕士, 副主任医师, 研究方向:哮喘病学。Tel:13325866303 E-mail: kfiqp@163.com
基金资助:
浙江省卫生厅科研基金资助项目(2007-B238); 台州市黄岩区科研基金资助项目(06081)

Effect of budesonide on the expression of inducible nitric oxide synthase at blood heterophil granulocyte in asthma rats

YE Hui¹, YING Xiao-ming¹, TONG Xia-sheng², CHEN Li-zhong¹, ZHAO Bei¹

¹Department of Pediatrics, Taizhou First People's Hospital, Taizhou 318020, Zhejiang, China;
²Department of Pediatrics, Taizhou Integrated Traditional and Western Medicine Hospital, Wenling 317523, Zhejiang, China

Received:2009-04-24 Revised:2009-08-11 Published:2020-11-03

摘要/Abstract

摘要： 目的: 观察诱导型一氧化氮合酶(iNOS) 蛋白在哮喘大鼠血中性粒细胞(PMN) 中的表达, 探讨PMN 参与哮喘炎症的可能机制, 并研究布地奈德的影响。方法: 采用卵白蛋白(OVA) 和Al(OH) 3 制备大鼠哮喘模型, 分离纯化血PMN, 免疫细胞化学法检测PMN 中iNOS 蛋白的表达水平,比色法测定支气管肺泡灌洗液(BALF) 中NO 浓度。结果: 哮喘组大鼠PMN 中iNOS 蛋白的表达水平显著高于正常对照组(P <0.01), 布地奈德治疗组PMN 中iNOS 蛋白的表达水平显著低于哮喘组(P <0.01) ;哮喘组支气管壁iNOS 蛋白的表达水平显著高于正常对照组(P <0.01), 布地奈德治疗组支气管壁iNOS 蛋白的表达水平显著低于哮喘组且高于正常对照组(均P <0.01) ;哮喘组BALF 中NO 浓度显著高于正常对照组(P <0.01), 布地奈德治疗组BALF 中NO 浓度显著低于哮喘组(P <0.01) ;PMN 中iNOS 蛋白的表达水平与BALF 中NO 浓度呈显著正相关(n =29, r =0.754, P <0.01) ;支气管壁iNOS 蛋白的表达水平与BALF 中NO 浓度呈显著正相关(n =29, r =0.760, P <0.01) 。结论: 哮喘大鼠PMN 合成iNOS蛋白的功能增加, PMN 可能部分通过iNOS 参与哮喘的发病, 这种功能可以部分被布地奈德所抑制。

关键词: 哮喘, 诱导型一氧化氮合酶, 一氧化氮, 中性粒细胞, 大鼠

Abstract: AIM: To observe the effect of budesonide on the expression of inducible nitric oxide synthase (iNOS) at blood polymorphonuclear leukocyte (PMN) in asthma rats. And to approach the pathogenesis of PMN in asthma inflammation. METHODS: The model rats of asthma were induced by OVA and Al (OH) 3.The blood PMN was isolated and purified. The expressions of iNOS protein at blood PMN were detected by immunohistochemical method. The concentration of nitric oxide (NO) at bronchoalveolar lavage fluids (BALF) were detected by colorimetric method. RESULTS: Expressions of iNOS protein at PMN in asthma group were significantly higher than those in control group (P <0.01). And the expressions of iNOS protein at PMN in budesonide treated group were significantly lower than those in asthma group (P < 0.01). The expressions of iNOS protein at bronchial wall in asthma group were significantly higher than those in control group (P <0.01). And the expressions of iNOS protein in budesonide treated group were significantly lower than those in asthma group, but higher than those in control group (all P <0.01). Concentrations of NO at BALF in asthma group were significantly higher than those in control group (P < 0.01). Concentrations of NO at BALF in budesonide treated group were significantly lower than those in asthma group. There was significant positive correlation between the expressions of iNOS protein at blood PMN and NO concentration at BALF(n =29, r =0.754, P <0.01). And there was significant positive correlation between the expressions of iNOS protein at bronchial wall and NO concentration at BALF (n =29, r = 0.760, P <0.01). CONCLUSION: The levels of iNOS protein synthesized by PMN are increased in asthma rats. PMN may be implicated with asthma pathogenesis via iNOS, but this function of PMN can be partly inhibited by budesonide.

Key words: asthma, inducible nitric oxide synthase, nitric oxide, polymorphonuclear leukocyte, rat

中图分类号:

R562.2

叶辉, 应小明, 童夏生, 陈利中, 赵蓓. 布地奈德对哮喘大鼠中性粒细胞诱导型一氧化氮合酶表达的影响[J]. 中国临床药理学与治疗学, 2009, 14(9): 1008-1012.

YE Hui, YING Xiao-ming, TONG Xia-sheng, CHEN Li-zhong, ZHAO Bei. Effect of budesonide on the expression of inducible nitric oxide synthase at blood heterophil granulocyte in asthma rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(9): 1008-1012.

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