肉苁蓉总苷对四氯化碳致小鼠急性肝损伤的保护作用

中国临床药理学与治疗学 ›› 2010, Vol. 15 ›› Issue (12): 1358-1361.

肉苁蓉总苷对四氯化碳致小鼠急性肝损伤的保护作用

罗慧英, 杨焕, 朱丽娟, 高忠清, 杨海林

甘肃中医学院药理教研室,兰州 730000,甘肃

收稿日期:2010-09-06 修回日期:2010-12-15 出版日期:2010-12-26 发布日期:2020-09-16
作者简介:罗慧英,女,博士,副教授,硕士生导师,研究方向:保肝与抗肿瘤药物研究。Tel: 0931-8765395 E-mail: louria @126.com

Protective effect of glycosides of Cistanches on CCl₄ damaged mice

LUO Hui-ying, YANG Huan, ZHU Li-juan, GAO Zhong-qing, YANG Hai-lin

Department of Pharmacology, Gansu College of Traditional Chinese Medicine, Lanzhou 730000, Gansu, China

Received:2010-09-06 Revised:2010-12-15 Online:2010-12-26 Published:2020-09-16

摘要/Abstract

摘要： 目的: 本研究旨在初步探讨肉苁蓉总苷对四氯化碳(CCl₄)所致小鼠急性肝损伤的保护作用。方法: 小鼠40只随机分为4组,分别为空白组、模型组、肉苁蓉总苷大、小剂量组(62.5、125 mg·kg^-1·d^-1),CCl₄花生油溶液灌胃建立小鼠急性肝损伤模型,每天灌胃给药2次,连续 14 d 后,检测肝功能生化酶指标及肝脏组织病理学变化。结果: 小鼠经CCl₄损伤后,血清AST、ALT和碱性磷酸酶(ALP)水平增高,肝脏细胞也出现了明显坏死;肉苁蓉总苷能有效地降低肝功指标,缓解组织细胞坏死症状(P<0.05)。结论: 肉苁蓉总苷对CCl₄所致小鼠急性肝损伤有明显的保护作用。

关键词: 肉苁蓉总苷, 四氯化碳, ALT, AST, 碱性磷酸酶

Abstract: AIM: To investigate the protective effect of glycosides of Cistanches (GCs) on CCl₄ damaged mice.METHODS: 40 mice were randomly divided into 4 groups, control group,CCl₄ model group,Cistanches groups (62.5,125 mg·kg^-1·d^-1). The acute liver damage mode was established by CCl₄ peanut oil in mice. The mice were received intragastric administration twice a day. After 14 d,the effect of GCs on ALT, AST, ALP and pathological changes were detected.RESULTS: GCs could obviously reduce the levels of ALT, AST and ALP in CCl₄ damaged mice, as well as moderating hepatocyte cytoclasis.CONCLUSION: GCs has protective effect on CCl₄ damaged mice.

Key words: Glycosides of Cistanches, CCl₄, ALT, AST, ALP

中图分类号:

R965.2

罗慧英, 杨焕, 朱丽娟, 高忠清, 杨海林. 肉苁蓉总苷对四氯化碳致小鼠急性肝损伤的保护作用[J]. 中国临床药理学与治疗学, 2010, 15(12): 1358-1361.

LUO Hui-ying, YANG Huan, ZHU Li-juan, GAO Zhong-qing, YANG Hai-lin. Protective effect of glycosides of Cistanches on CCl₄ damaged mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(12): 1358-1361.

参考文献

[1] 李琳琳,王晓雯,王雪飞,等.肉苁蓉总甙的抗脂质过氧化作用及抗辐射作用[J].中国中药杂志,1997,22(6): 364-367.
[2] 皋聪,王传社,巫冠中,等. 苁蓉总苷对血管性痴呆大鼠学习记忆的影响及机制研究[J]. 中草药,2005, 36(12):1852-1855.
[3] 商小英,王晓雯,王雪飞,等. 肉苁蓉总苷对60Coγ射线损伤小鼠免疫功能的防护作用[J]. 中草药,2001, 32(2):139-142.
[4] 蒋晓燕,王晓雯,商小英. 肉苁蓉总苷对60Coγ射线照射小鼠脾损伤的影响[J]. 新疆医学科大学学报,2001, 24(4):297-299.
[5] 蒋晓燕,王晓雯,王雪飞,等.肉苁蓉总苷对60Coγ射线照射小鼠造血系统损伤保护作用的研究[J]. 中国药理学通报,2000, 16(3):332-335.
[6] 邬利娅,伊明,王晓雯,等.肉从蓉总苷对60Co照射损伤小鼠T淋巴细胞功能的影响[J]. 新疆医学科大学学报,2003, 26(6):558-560.
[7] 罗慧英,刘渊,席国柱,等.肉苁蓉总苷对小鼠酒精性肝损伤的保护作用[J].中国临床药理学与治疗学,2009, 14 (11) :1225 - 1228.
[8] Kaplowitz N. Mechanisms of liver cell injury[J]. J Hepatol, 2000,32(1) :39-47.
[9] Kaplowitz N.Cell death at the millennium. Implications for liver diseases[J]. Clin Liver Dis, 2000, 4(1): 1-23.
[10] Kaplowitz N.Biochemical and cellular mechanisms of toxic liver injury[J].Semin Liver Dis, 2002,22(2) :137-144.
[11] Adamson GM,Harman AW.Oxidative stress in cultured hepatocytes exposed to acetaminophen[J].Biochem Pharmacol , 1993,45(11) : 2289-2294.
[12] Kaplowitz N , Tsukamoto H. Oxidative stress and liver disease[J]. Prog Liver Dis, 1996,14 :131-159.
[13] Kedderis GL.Biochemical basis of hepatocellular injury[J]. Toxicol Pathol, 1996,24(1): 77-83.
[14] James LP, Mayeux PR, Hinson JA. Acetaminopheninduced hepatotoxicity[J]. Drug Metab Dispos, 2003,31(12) :1499-1506.
[15] Kaplowitz N. Drug-induced liver injury[J].Clin Infect Dis, 2004, 38(2): S44-S48.
[16] Pumford NR, Halmes NC, Hinson JA. Covalent binding of xenobiotics to specific proteins in the liver[J].Drug Metab Rev, 1997,29(1/2): 39-57.
[17] Brattin WJ, Glende EA Jr,Recknagel RO. Pathological mechanisms in carbon tetrachloride hepatotoxicity[J]. J Free Radic Bio Med,1985,(1): 27-38.
[18] Recknagel RO, Glende Jr EA, Britton RS.Free radical damage and lipid peroxidation[M]. In: Meeks, RG(Ed), Hepatotoxicology. CRC Press Florida, 1991:401-436.
[19] Williams AT, Burk RF. Carbon tetrachloride hepatotoxicity: an example of free radical-mediated injury[J]. Semin Liver Dis,1990, 10(4):279-284.
[20] Brent JA, Rumack BH. Role of free radicals in toxic hepatic injury 11. Are free radicals the cause of toxin-induced liver injury[J] ? J Toxicol Clin ,1993,31(1): 173-196.