大鼠创伤性脑损伤模型的建立

中国临床药理学与治疗学 ›› 2010, Vol. 15 ›› Issue (12): 1362-1367.

大鼠创伤性脑损伤模型的建立

顾兵¹, 金建波², 孟玮¹, 李华南²

¹江西科技师范学院生命科学学院,南昌 330013,江西;
²江西中医学院药学院,南昌 330006,江西

收稿日期:2010-11-02 修回日期:2010-12-08 出版日期:2010-12-26 发布日期:2020-09-16
作者简介:顾兵,男,博士后,副教授,硕士生导师,从事神经精神药物学研究。E-mail: bguemory@hotmail.com
基金资助:
江西省自然科学基金项目(2007GQY1578);江西省教育厅科技项目(GJJ10244);江西科技师范学院校级课题(KY2008ZY04)

Establishment of traumatic brain injury model in rats

GU Bing¹, JIN Jian-bo², MENG Wei¹, LI Hua-nan²

¹College of Life Science, Jiangxi Science & Technology Normal University, Nanchang 330013, Jiangxi, China;
²School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, Jiangxi,China

Received:2010-11-02 Revised:2010-12-08 Online:2010-12-26 Published:2020-09-16

摘要/Abstract

摘要： 目的: 建立一种控制性皮层撞击脑损伤大鼠模型。方法: 采用Benchmark^TM颅脑损伤撞击器制备大鼠创伤性脑损伤,并在伤后1、3、5、7、28 d 借助Morris水迷宫评价其认知功能,同时观察损伤部位及其邻近区域的形态学改变。结果: 脑损伤大鼠寻找隐藏平台所用时间显著高于假手术组。损伤部位及海马回可见神经细胞肿胀、坏死,胶质细胞明显增生。经铜银染色可见神经元及其轴突溃变。结论: 建立的大鼠控制性皮层撞击脑损伤模型重复性好,适合脑保护药物的药效学筛选。

关键词: 创伤性脑损伤, 动物模型, 大鼠, 铜银染色

Abstract: AIM: To establish a rat model for controlled cortical impact brain injury.METHODS: Traumatic brain injury(TBI) model in rats was duplicated by controlled cortical impact brain injury method with a Benchmark^TM stereotaxic impactor.Cognitive function was evaluated at 1, 3, 5, 7, 28 day after TBI with Morris water maze. And then morphological changes was carefully inspected in the injured part and adjacent area.RESULTS: Compared with the sham-operated rats, the time for brain injured rats to find hidden platform were significantly prolonged. Neuronal cells were swollen or loss, and glia cells remarkably increased in the injured sites and hippocampus areas.Neuron and its axon degeneration was observed by using curpric-silver straining technique.CONCLUSION: The rat model for controlled cortical impact brain injury is reproducible and suitable for the pharmacodynamic screening of brain protective drugs.

Key words: Taumatic brain injury, Animal model, Rat, Cupric-silver staining

中图分类号:

顾兵, 金建波, 孟玮, 李华南. 大鼠创伤性脑损伤模型的建立[J]. 中国临床药理学与治疗学, 2010, 15(12): 1362-1367.

GU Bing, JIN Jian-bo, MENG Wei, LI Hua-nan. Establishment of traumatic brain injury model in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(12): 1362-1367.

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