左氧沙星与磷霉素联用对金黄色葡萄球菌的体外杀菌活性研究

中国临床药理学与治疗学 ›› 2010, Vol. 15 ›› Issue (12): 1388-1394.

左氧沙星与磷霉素联用对金黄色葡萄球菌的体外杀菌活性研究

宋秀杰¹, 鞠红梅², 于旭红³, 林德峰³, 王睿³, 刘又宁¹

¹解放军总医院呼吸科,北京 100853;
²吉林油田总医院,松原 138000,吉林;
³解放军总医院临床药理研究室,北京 100853

收稿日期:2010-08-27 修回日期:2010-11-29 出版日期:2010-12-26 发布日期:2020-09-16
作者简介:宋秀杰,女,主治医师,研究方向:肺部感染疾病的诊断和治疗。Tel: 66958502 E-mail: sxjsxj@163.com

In vitro activities of levofloxacin in combination with fosfomycin against staphylococcus aureus

SONG Xiu-jie¹, JU Hong-mei², YU Xu-hong³, LIN De-feng³, WANG Rui³, LIU You-ning¹

¹Respiratory Department, Chinese PLA General Hospital, Beijing 100853,China;
²Jilin Oil Field General Hospital of Songyuan, Songyuan 138000, Jilin, China;
³Deptartment of Clinical Pharmacology, Chinese PLA General Hospital, Beijing 100853, China

Received:2010-08-27 Revised:2010-11-29 Online:2010-12-26 Published:2020-09-16

摘要/Abstract

摘要： 目的: 评价左氧沙星与磷霉素联用对金黄色葡萄球菌的联合抗菌效应。了解左氧沙星单用及与磷霉素联用的杀菌曲线特点。方法: 选取3株MSSAATCC29213、临床分离菌SA99、SARN450(实验室菌株)和1株临床分离的MRSAB6177,采用菌落计数法对不同浓度的左氧沙星或磷霉素单用及两药联合对3株MSSA和1株MRSA进行体外杀菌实验。结果: 左氧沙星的杀菌曲线表现出浓度依赖性,磷霉素的杀菌曲线表现出非浓度依赖性。两药1/4MIC浓度单用时虽然均无法抑制MSSA的生长,而两药亚抑菌浓度联合使用后即表现出协同效应和杀菌效应。两药4MIC、8MIC联合时对MRSA表现出杀菌效应。结论: 亚抑菌浓度的左氧沙星与磷霉素联合对MSSA即可出现协同杀菌效应。左氧沙星联合磷霉素对MRSA也表现出显著的杀菌活性。

关键词: 金黄色葡萄球菌, 左氧沙星, 磷霉素, 杀菌曲线

Abstract: AIM: To investigate the in vitro bactericidal activity of levofloxacin alone and combined with fosfomycin against 3 strains MSSA and 1 stain MRSA.METHODS: Three MSSA strains and 1 MRSA from clinical specimens were used in this study. Time-kill curve method was carried out with different concentration of the two drugs both alone and in combination.RESULTS: Levoloxacin alone showed a concentration-dependent time-kill curve against three strains of MSSA, fosfomycin showed a nonconcentration-dependent time-kill curve. Neither levofloxacin nor fosfomycin alone inhibited bacterial growth at 1/4 MIC; but the combination of this two drugs at 1/4 the respective MIC showed a synergistic bactericidal effect, a 2-3 log₁₀ decrease with respect to the most active antibiotic alone.CONCLUSION: The combination of subinhibitory concentrations of levofloxacin and fosfomycin presented synergism,exerting a bactericidal effect. The combination also has a bactericidal effect against MRSA.

Key words: Staphylococcus aureus, Levofloxacin, Fosfomycin , Time-kill curve

中图分类号:

R965.2

宋秀杰, 鞠红梅, 于旭红, 林德峰, 王睿, 刘又宁. 左氧沙星与磷霉素联用对金黄色葡萄球菌的体外杀菌活性研究[J]. 中国临床药理学与治疗学, 2010, 15(12): 1388-1394.

SONG Xiu-jie, JU Hong-mei, YU Xu-hong, LIN De-feng, WANG Rui, LIU You-ning. In vitro activities of levofloxacin in combination with fosfomycin against staphylococcus aureus[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(12): 1388-1394.

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