谷氨酰胺对肠穿孔重症感染模型的治疗作用

摘要/Abstract

摘要： 目的: 探讨补充谷氨酰胺(莱美活力)对实验性肠穿孔所致重症感染的治疗作用及与抗生素疗效的比较。方法: 50只健康雌性SD大鼠随机分为5组:假手术组,模型组,谷氨酰胺组,头孢孟多组,联合治疗组。假手术组进行假手术,其它组通过盲肠结扎穿孔手术(CLP)建立急性肠穿孔所致重症感染大鼠模型。假手术组和模型组分别给予生理盐水腹腔注射,其它组分别给予谷氨酰胺、头孢孟多酯钠及二者合用腹腔注射。观察实验大鼠的腹腔炎症情况,小肠组织大体及镜下改变,自动血常规仪测外周血细胞,鲎试剂法测血清内毒素、ELISA法测血清磷脂酶A₂(PLA₂)。结果: 模型组大鼠血清学指标较假手术组明显增高(P<0.01),各处理组较模型组不同程度下降(P＜0.01)。结论: 补充谷氨酰胺对实验性急性肠穿孔所致重症感染具有治疗作用,其疗效与头孢孟多具有可比性,两者合用有协同治疗作用。

关键词: 谷氨酰胺, 重症感染, 肠穿孔, 内毒素, 磷脂酶A₂

Abstract: AIM: To explore the effects of glutamine on experimental enterobrosis and severe infection in SD rats and compared to antibiotics. METHODS: 50 healthy female SD rats were randomly divided into five groups: sham operation group, model group, glutamine group(N(2)-L-Alanyl-L-glutamine), Cefamandole group, combined treatment group(N(2)-L-Alanyl-L-glutamine + Cefamandole sodium). Sham surgery were performed on sham operation group, experimental enterobrosis and severe infection rats model were established on other groups with CLP surgery. Control and model group received intraperitoneal injection of saline, other groups were treated with corresponding drugs. We examined the infection of abdominal cavity, blood cells, the serum concentration of lipopolysaccharide and phospholipase A₂. RESULTS: The index of model group were higher than control group(P<0.01), and the index of glutamine group, Cefamandole group, combined treatment group by N(2)-L-Alanyl-L-glutamine and Cefamandole sodium were lower than model group(P<0.01). CONCLUSION: Supplementary of glutamine is effective in treating severe infection caused by experimental enterobrosis and the effects is comparative with antibiotics. The combined treatment shows synergistic effect in treatment of enterobrosis and severe infection.

Key words: Glutamine, Severe infection, Enterobrosis, Endotoxin, Phospholipase A₂

中图分类号:

范晓, 赫留党, 徐杨. 谷氨酰胺对肠穿孔重症感染模型的治疗作用[J]. 中国临床药理学与治疗学, 2010, 15(4): 410-415.

FAN Xiao, HE Liu-dang, XU Yang. Effects of glutamine on experimental enterobrosis and severe infection treatment[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(4): 410-415.

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