心肌缺血及再灌注大鼠血浆蛋白C活性变化的实验研究

中国临床药理学与治疗学 ›› 2010, Vol. 15 ›› Issue (9): 1012-1015.

心肌缺血及再灌注大鼠血浆蛋白C活性变化的实验研究

包丽丽¹, 张根葆^1,2, 张世¹, 吴娟²

¹皖南医学院病理生理学教研室, ²蛇毒研究室,芜湖 241002,安徽

收稿日期:2010-07-14 修回日期:2010-08-12 出版日期:2010-09-26 发布日期:2020-09-17
通讯作者: 张根葆,男,教授,硕士生导师,研究方向:蛇毒毒理学、心血管病理生理学。Tel: 13956167066, E-mail: zgb858@163.com
作者简介:包丽丽,女,硕士研究生,研究方向:心血管病理生理学。Tel: 15212232676, E-mail:qfly5201314@sohu.com
基金资助:
安徽省高教青年教师资助计划基金项目(2007JQ1172)

Experimental study on the changes of protein C activity in plasma with myocardial ischemia and reperfusion in rats

BAO Li-li¹, ZHANG Gen-bao^1,2, ZHANG Shi¹, Wu Juan²

¹Department of Pathophysiology, ²Venom Research Room, Wannan Medical College, Wuhu 241002,Anhui,China

Received:2010-07-14 Revised:2010-08-12 Online:2010-09-26 Published:2020-09-17

摘要/Abstract

摘要： 目的: 探讨心肌缺血及再灌时大鼠血浆蛋白C(PC)活性及血小板聚集率的变化和意义。方法: SD雄性大鼠40只,随机分为对照组(C,n=8)、缺血组(I,n=24)和缺血再灌组(IR,n=8),缺血组按照造模后缺血时间的不同又分别分为缺血 10 min(I₁₀)、30 min(I₃₀)及60 min(I₆₀)组,每组8只。采用可逆性阻断大鼠左冠状动脉前降支的方法制备心肌缺血及再灌注模型,实验结束后即刻颈总动脉取血,以发色底物法检测血浆蛋白C活性,比浊法测定血小板聚集率。结果: 心肌缺血模型组大鼠血浆PC活性均明显低于对照组(P<0.01), 且随心肌缺血时间延长降低;缺血再灌组PC活性略有恢复,但仍低于对照组(P<0.01)。血小板聚集率缺血 10 min、缺血 30 min 组及IR组较对照组均明显升高(P<0.01),而缺血 60 min 组血小板聚集率与对照组比较无统计学意义。结论: 心肌缺血及再灌注损伤可引起PC活性下降,PC活性的变化参与了心肌缺血及损伤的过程,较血小板聚集率敏感。因此,PC活性的变化有可能成为对评价缺血性心血管疾病有预报价值的指标。

关键词: 蛋白C, 心肌缺血再灌注损伤, 血小板聚集率

Abstract: AIM: To investigate the clinical significance of plasma levels of protein C(PC) and platelet aggregation in rats with myocardial ischemia and reperfusion injury.METHODS: 40 male SD rats were randomly divided into groups of control (C,n=8),ischemia(I,n=24) and ischemia-reperfusion(IR,n=8). The ischemia groups were divided into 10 min (I₁₀),30 min (I₃₀) and 60 min group(I₆₀) respectively, 8 rats in each group. The rat model of myocardial ischemia and reperfusion was prepared by the left anterior descending coronary artery reversible occlusion. Blood samples were collected from the carotid artery immediately. The plasma PC activity and platelet aggregation were detected by chromogenic substrate assay and turbidity assay, respectively. RESULTS: Plasma PC activities in myocardial ischemia rats were significantly lower than those in the control (P<0.05),and there is a negative relation between the decrease of PC activity and ischemia time;plasma PC activities in ischemia-reperfusion rats were slightly recovered, but still lower than those in the control (P<0.01). The platelet aggregation in ischemia 10 min,30 min ischemia and IR rats were significantly higher than those in the control (P<0.01), while ischemia 60 min platelet aggregation showed no statistical significance compared with the control group.CONCLUSION: Myocardial ischemia and reperfusion injury can result in PC activities loss. These suggest that the decrease of PC activity may be involved in myocardial ischemia and injury, more sensitive than platelet aggregation. Therefore, the PC activity maybe a predictive indicator for ischemic cardiovascular disease.

Key words: Protein C, Myocardial ischemia and reperfusion injury, Platelet aggregation

中图分类号:

R965.1

包丽丽, 张根葆, 张世, 吴娟. 心肌缺血及再灌注大鼠血浆蛋白C活性变化的实验研究[J]. 中国临床药理学与治疗学, 2010, 15(9): 1012-1015.

BAO Li-li, ZHANG Gen-bao, ZHANG Shi, Wu Juan. Experimental study on the changes of protein C activity in plasma with myocardial ischemia and reperfusion in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(9): 1012-1015.

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