微透析技术用于化疗药物——吉西他滨体内药动学的初步研究

中国临床药理学与治疗学 ›› 2010, Vol. 15 ›› Issue (9): 1035-1039.

微透析技术用于化疗药物——吉西他滨体内药动学的初步研究

谢波¹, 凌家俊², 吴秀君², 付湘², 黄雪琴¹

¹广州军区广州总医院肿瘤科,广州 510010,广东;
²广州中医药大学中药学院,广州 510006,广东

收稿日期:2010-08-08 修回日期:2010-09-15 出版日期:2010-09-26 发布日期:2020-09-17
通讯作者: 凌家俊,男,教授,中药学博士,从事药剂学及药物动力学相关研究。Tel: 020-39358043, E-mail: piglion88@gmail.com
作者简介:谢波,女,副主任医师,医学硕士,从事肿瘤学临床及相关基础研究。
基金资助:
广东省医学科研基金项目(A2007481);国家自然科学基金项目(30772791);广东省科技计划项目(2010B030700035)

Pharmacokinetic reseach of anti-cancer drug-Gemcitabine in vivo model by microdialysis technique

XIE Bo¹, LING Jia-jun², WU Xiu-jun², FU Xiang², HUANG Xue-qin¹

¹Department of Oncology, General Military Hospital of Guangzhou, Guangzhou 510010, Guangdong,China;
²College of Pharmacy, Guangzhou University of Chinese Medicine, Guangzhou 510006,Guangdong, China

Received:2010-08-08 Revised:2010-09-15 Online:2010-09-26 Published:2020-09-17

摘要/Abstract

摘要： 目的: 建立以微透析法(microdialysis, MD)为采样技术的肿瘤化疗药物在体检测方法,并进行药动学研究。方法: 以吉西他滨(GEM)为研究对象,大鼠为实验动物,采用尾静脉注射为给药方式,通过微透析技术进行血管内取样,对血药浓度进行在线、实时、连续监测,求算相关药动学参数。结果: GEM在大鼠体内血液中的探针回收率为(11.9±2.0)%,经大鼠尾静脉给药后GEM体内过程为二室模型,其消除和分布为一级动力学过程。实验过程中大鼠未见明显副作用。结论: 微透析技术可用于活体动物体内GEM浓度的连续监测,提示微透析技术可用于抗肿瘤药物的局部药动学研究。

关键词: 微透析, 吉西他滨, 肿瘤, 药动学

Abstract: AIM: To estabalish the methodology of microdialysis technique in pharmacokinetic(PK) reseach of anticancer drugs in vivo.METHODS: Gemcitabine (GEM) was injected into mice's tail vein. The microdialysate samples from blood were collected after dosing in gemcitabine-treated mices. The concentration of GEM was detected real-time continuously and pharmacokinetic parameters were evaluated.RESULTS: The recovery rate of GEM in vivo was (11.9±2.0)%. It showed that the pharmacokinetics of gemcitabine was two-compartment model in vivo and the elimination and distribution of GEM meets the first kinetics. There were no serious side effects in model mice.CONCLUSION: Microdialysis can be successfully employed in living body to detect the concentration of GEM continuously, which prompts it is possible to study the PK of anticancer drugs in malignant tissues in vivo.

Key words: Microdialysis, Gemcitabine, Anticancer drugs, Pharmacokinetic

中图分类号:

谢波, 凌家俊, 吴秀君, 付湘, 黄雪琴. 微透析技术用于化疗药物——吉西他滨体内药动学的初步研究[J]. 中国临床药理学与治疗学, 2010, 15(9): 1035-1039.

XIE Bo, LING Jia-jun, WU Xiu-jun, FU Xiang, HUANG Xue-qin. Pharmacokinetic reseach of anti-cancer drug-Gemcitabine in vivo model by microdialysis technique[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(9): 1035-1039.

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