四妙散加味方对人肾小管上皮细胞UAT和URAT1基因表达的影响

中国临床药理学与治疗学 ›› 2010, Vol. 15 ›› Issue (9): 972-977.

四妙散加味方对人肾小管上皮细胞UAT和URAT1基因表达的影响

王华杰¹, 张国华¹, 邹震², 范秀珍¹

¹邵阳市第一人民医院,邵阳 422000,湖南;
²湖南省中医研究院附属医院,长沙 410008,湖南

收稿日期:2010-07-08 修回日期:2010-08-25 出版日期:2010-09-26 发布日期:2020-09-17
作者简介:王华杰,男,本科,副主任医师,主要从事中医风湿临床治疗及研究。Tel: 13574967941, E-mail: fanxiuz@sina.com
基金资助:
2008年度湖南省邵阳市科研计划资助项目(53N08)

Effects of SiMiaoSanJiaWeiFang on the expression of human UAT and URAT1 transporter genes in HK-2 cells

WANG Hua-jie¹, ZHANG Guo-hua¹, ZHOU-Zhen², FAN Xiu-zhen¹

¹First People's Hospital of Shaoyang City, Shaoyang 422000, Hunan,China;
²Affiliated Hospital of Traditional Chinese Medicine Research Institute, Changsha 410008, Hunan,China

Received:2010-07-08 Revised:2010-08-25 Online:2010-09-26 Published:2020-09-17

摘要/Abstract

摘要： 目的: 观察低、高剂量四妙散加味方(SMSJWF)是否调节人肾小管上皮细胞(HK-2细胞)尿酸盐转运子(hUAT)mRNA和有机阴离子转运体(hURAT1)mRNA的表达。方法: 根据培养液中所含四妙散加味方剂量的不同,将HK-2细胞分为:DMEM/F-12+6 mL/2 kg 四妙散加味方低剂量组;DMEM/F-12+12 mL/2 kg 四妙散加味方高剂量组;DMEM/F-12+苯溴马隆4.8 mg/d 阳性对照组;仅使用DMEM/F-12+空白血清对照组。四组细胞分别培养48 h,采用实时荧光定量PCR检测HK-2细胞中hUAT mRNA、hURAT1 mRNA的相对表达量(2^△△Ct法)。结果: 所有标本均能检测到hUAT mRNA和hURAT1 mRNA的表达。低、高剂量组和阳性对照组hUAT mRNA表达水平均明显高于空白对照组(P＜0.05);低剂量和阳性对照组的hURAT1 mRNA的表达明显低于空白对照组(P＜0.05),而高剂量组hURAT1 mRNA的表达明显高于其他各组(P＜0.05)。结论: 低、高剂量四妙散加味方可上调hUAT mRNA表达;低剂量四妙散加味方与苯溴马隆均可下调hURAT1 mRNA的表达,这可能是其降尿酸的作用机制之一。

关键词: 四妙散加味方, 尿酸, 肾小管, 基因表达, 转运蛋白

Abstract: AIM: To observe the effect of SiMiaoSanJiaWeiFang(SMSJWF) in different concentrations on the expression of human UAT and URAT1 transporter genes in HK-2 cells.METHODS: HK-2 cells were divided into 4 groups as follows: The low dosage group (cultured with DMEM/F-12 and 6 mL/2 kg SMSJWF ); the high dosage group (cultured with DMEM/F-12 and 12 mL/2 kg SMSJWF ); the positive control group(cultured with DMEM/F-4.8 mg/d Benzbromarone ); the blank control group(cultured with DMEM/F-12). In each group, 6 bottles of the cells were cultured in vitro for 48 hours. Then the relative quantity of hUAT and hURAT1 mRNA in the HK-2 cells was detected by real-time fluorescent quantitative PCR assay.RESULTS: hUAT and hURAT1 mRNA could be detected in all samples. And the level of hUAT mRNA in the blank control group were significantly lower than those in the other groups (P＜0.05). Compared with blank control group, the expressions of hURAT1 mRNA in low dosage group and Benzbromarone group were reduced clearly (P＜0.05). By contrast, the expression in high dosage group was increased clearly (P＜0.05).CONCLUSION: SiMiaoSanJiaWeiFang up-regulates the expression of hUAT gene. SiMiaoSanJiaWeiFang down-regulates the expression of hURAT1 gene, which may be one of the mechanism to reduce uric acid levels.

Key words: SiMiaoSanJiaWeiFang, Uric acid, Kidney tubules, Gene expression, Carrier proteins

中图分类号:

R311
R965.2

王华杰, 张国华, 邹震, 范秀珍. 四妙散加味方对人肾小管上皮细胞UAT和URAT1基因表达的影响[J]. 中国临床药理学与治疗学, 2010, 15(9): 972-977.

WANG Hua-jie, ZHANG Guo-hua, ZHOU-Zhen, FAN Xiu-zhen. Effects of SiMiaoSanJiaWeiFang on the expression of human UAT and URAT1 transporter genes in HK-2 cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(9): 972-977.

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